Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes
The flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth...
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2011-06-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/1392 |
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| author | S. V. Ryzhov K. S. Yuryeva K. V. Goremykin Ye. V. Korotkaya I. V. Saltykova Yu. A. Yakovleva Ye. S. Kulikov O. S. Fyodorova Ye. E. Kremer N. S. Fattakhov A. E. Sazonov |
| author_facet | S. V. Ryzhov K. S. Yuryeva K. V. Goremykin Ye. V. Korotkaya I. V. Saltykova Yu. A. Yakovleva Ye. S. Kulikov O. S. Fyodorova Ye. E. Kremer N. S. Fattakhov A. E. Sazonov |
| author_sort | S. V. Ryzhov |
| collection | DOAJ |
| description | The flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) in monocytes were determined using real-time PCR. We found considerable variation across individuals in mRNA expression levels of paracrine factors after the stimulation of adenosine receptors. Our findings suggests the role of adenosinergic system accounted for interindividual differences in monocyte activation. |
| format | Article |
| id | doaj-art-a2772ef9bab5410da37349c1c2cb575d |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2011-06-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-a2772ef9bab5410da37349c1c2cb575d2025-08-20T04:00:19ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842011-06-01103546110.20538/1682-0363-2011-3-54-61904Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytesS. V. Ryzhov0K. S. Yuryeva1K. V. Goremykin2Ye. V. Korotkaya3I. V. Saltykova4Yu. A. Yakovleva5Ye. S. Kulikov6O. S. Fyodorova7Ye. E. Kremer8N. S. Fattakhov9A. E. Sazonov10Университет ВандербилтаСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетThe flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) in monocytes were determined using real-time PCR. We found considerable variation across individuals in mRNA expression levels of paracrine factors after the stimulation of adenosine receptors. Our findings suggests the role of adenosinergic system accounted for interindividual differences in monocyte activation.https://bulletin.ssmu.ru/jour/article/view/1392monocyteadenosineadenosine receptorsparacrine factorsinterleukin 6interleukin 8vascular endothelial growth factor |
| spellingShingle | S. V. Ryzhov K. S. Yuryeva K. V. Goremykin Ye. V. Korotkaya I. V. Saltykova Yu. A. Yakovleva Ye. S. Kulikov O. S. Fyodorova Ye. E. Kremer N. S. Fattakhov A. E. Sazonov Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes Бюллетень сибирской медицины monocyte adenosine adenosine receptors paracrine factors interleukin 6 interleukin 8 vascular endothelial growth factor |
| title | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_full | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_fullStr | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_full_unstemmed | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_short | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_sort | adenosine dependent regulation of paracrine factors expression in human venous blood monocytes |
| topic | monocyte adenosine adenosine receptors paracrine factors interleukin 6 interleukin 8 vascular endothelial growth factor |
| url | https://bulletin.ssmu.ru/jour/article/view/1392 |
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