Optimization of the Encapsulation of Lemuru Fish Protein Hydrolysate by Spray-Drying Using Response Surface Methodology

Research background. Encapsulating lemuru fish protein hydrolysate is important to maintain its stability. However, optimal conditions for the encapsulation process of lemuru fish protein hydrolysate using statistical methods remain unexplored. This study aims to address this problem by optimizing t...

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Main Authors: Ayu Hanifah, Wawan Kosasih, Diah Ratnaningrum, Dian Andriani, Herlian Eriska Putra, Yellianty Yelliantty, Sri Priatni
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2025-01-01
Series:Food Technology and Biotechnology
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Online Access:https://hrcak.srce.hr/file/477751
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author Ayu Hanifah
Wawan Kosasih
Diah Ratnaningrum
Dian Andriani
Herlian Eriska Putra
Yellianty Yelliantty
Sri Priatni
author_facet Ayu Hanifah
Wawan Kosasih
Diah Ratnaningrum
Dian Andriani
Herlian Eriska Putra
Yellianty Yelliantty
Sri Priatni
author_sort Ayu Hanifah
collection DOAJ
description Research background. Encapsulating lemuru fish protein hydrolysate is important to maintain its stability. However, optimal conditions for the encapsulation process of lemuru fish protein hydrolysate using statistical methods remain unexplored. This study aims to address this problem by optimizing the encapsulation conditions. Experimental approach. Maltodextrin and gum Arabic were used as carrier agents, with mass per volume ratio ranging from 10 to 30 %, and spray dryer inlet temperatures between 90 and 100 °C. In this study, we analysed the main interactions of these variables using response surface methodology (RSM). Results and conclusions. Our results show that mass per volume ratio of maltodextrin of 25 % and inlet temperature of 100 °C are the optimal conditions for the encapsulation of fish protein hydrolysate. The optimal conditions resulted in a high desirability index of 0.864, indicating an effective balance between yield, solubility and hygroscopicity. The actual results also fall well within the confidence interval of the predicted values, confirming the robustness of the model and the reliability of the predicted optimal encapsulation conditions. Encapsulated fish protein hydrolysate was compared with its non-encapsulated counterpart and characterised using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and particle size analyser (PSA) to validate the results. The encapsulated fish protein hydrolysate showed distinct properties, such as the presence of functional groups from maltodextrin, interconnected particle and more homogeneous and narrower particle size distribution. Novelty and scientific contribution. The encapsulation of lemuru fish protein hydrolysate using maltodextrin with mass per volume ratio of 25 % and inlet temperature 100 °C was successful in improving the properties of the protein hydrolysate. Further research should explore the functional properties of fish protein hydrolysate.
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publishDate 2025-01-01
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spelling doaj-art-a274fcef7af041df85ec470ba070e0102025-08-20T03:49:12ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062025-01-01631839310.17113/ftb.63.01.25.8626Optimization of the Encapsulation of Lemuru Fish Protein Hydrolysate by Spray-Drying Using Response Surface MethodologyAyu Hanifah0Wawan Kosasih1Diah Ratnaningrum2Dian Andriani3Herlian Eriska Putra4Yellianty Yelliantty5Sri Priatni6Research Center for Applied Microbiology, National Research and Innovation Agency Republic of Indonesia, Jl. Raya Jakarta-Bogor Km, 46, Cibinong, Bogor, Jawa Barat 16911, IndonesiaResearch Center for Applied Microbiology, National Research and Innovation Agency Republic of Indonesia, Jl. Raya Jakarta-Bogor Km, 46, Cibinong, Bogor, Jawa Barat 16911, IndonesiaDirectorate of Laboratory Management, Research Facilities and Science and Technology areas, KST Samaun Samadikun, Gd Basics Tw 1, Lt 1, Cisitu-Sangkuriang, Bandung 40135, IndonesiaResearch Center for Environment and Clean Technology, National Research and Innovation Agency Republic of Indonesia, KST BJ Habiebie Gd. 720 Setu Tangerang Selatan Banten 15314, IndonesiaResearch Center for Environment and Clean Technology, National Research and Innovation Agency Republic of Indonesia, KST BJ Habiebie Gd. 720 Setu Tangerang Selatan Banten 15314, IndonesiaDepartment of Food Technology, Faculty of Engineering, Universitas Pasundan Jalan Setiabudi 193 Bandung, West Java 40135, IndonesiaResearch Center for Applied Microbiology, National Research and Innovation Agency Republic of Indonesia, Jl. Raya Jakarta-Bogor Km, 46, Cibinong, Bogor, Jawa Barat 16911, IndonesiaResearch background. Encapsulating lemuru fish protein hydrolysate is important to maintain its stability. However, optimal conditions for the encapsulation process of lemuru fish protein hydrolysate using statistical methods remain unexplored. This study aims to address this problem by optimizing the encapsulation conditions. Experimental approach. Maltodextrin and gum Arabic were used as carrier agents, with mass per volume ratio ranging from 10 to 30 %, and spray dryer inlet temperatures between 90 and 100 °C. In this study, we analysed the main interactions of these variables using response surface methodology (RSM). Results and conclusions. Our results show that mass per volume ratio of maltodextrin of 25 % and inlet temperature of 100 °C are the optimal conditions for the encapsulation of fish protein hydrolysate. The optimal conditions resulted in a high desirability index of 0.864, indicating an effective balance between yield, solubility and hygroscopicity. The actual results also fall well within the confidence interval of the predicted values, confirming the robustness of the model and the reliability of the predicted optimal encapsulation conditions. Encapsulated fish protein hydrolysate was compared with its non-encapsulated counterpart and characterised using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and particle size analyser (PSA) to validate the results. The encapsulated fish protein hydrolysate showed distinct properties, such as the presence of functional groups from maltodextrin, interconnected particle and more homogeneous and narrower particle size distribution. Novelty and scientific contribution. The encapsulation of lemuru fish protein hydrolysate using maltodextrin with mass per volume ratio of 25 % and inlet temperature 100 °C was successful in improving the properties of the protein hydrolysate. Further research should explore the functional properties of fish protein hydrolysate.https://hrcak.srce.hr/file/477751encapsulationprotein hydrolysatelemuru fishspraydryingRSM
spellingShingle Ayu Hanifah
Wawan Kosasih
Diah Ratnaningrum
Dian Andriani
Herlian Eriska Putra
Yellianty Yelliantty
Sri Priatni
Optimization of the Encapsulation of Lemuru Fish Protein Hydrolysate by Spray-Drying Using Response Surface Methodology
Food Technology and Biotechnology
encapsulation
protein hydrolysate
lemuru fish
spraydrying
RSM
title Optimization of the Encapsulation of Lemuru Fish Protein Hydrolysate by Spray-Drying Using Response Surface Methodology
title_full Optimization of the Encapsulation of Lemuru Fish Protein Hydrolysate by Spray-Drying Using Response Surface Methodology
title_fullStr Optimization of the Encapsulation of Lemuru Fish Protein Hydrolysate by Spray-Drying Using Response Surface Methodology
title_full_unstemmed Optimization of the Encapsulation of Lemuru Fish Protein Hydrolysate by Spray-Drying Using Response Surface Methodology
title_short Optimization of the Encapsulation of Lemuru Fish Protein Hydrolysate by Spray-Drying Using Response Surface Methodology
title_sort optimization of the encapsulation of lemuru fish protein hydrolysate by spray drying using response surface methodology
topic encapsulation
protein hydrolysate
lemuru fish
spraydrying
RSM
url https://hrcak.srce.hr/file/477751
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