A Comparative Study for Various Morphological Characters between Diploid and Triploid Watermelon
The present study was carried out during May–July, 2015 at the High-tech seed testing laboratory under the Department of Vegetable Science, Kerala Agriculture University, Trissur, India to differentiate between the normal diploid(2x) watermelon var. Sugar Baby with the yellow seedless triploid(3x)...
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Puspa Publishing House
2022-08-01
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| Series: | International Journal of Economic Plants |
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| Online Access: | https://ojs.pphouse.org/index.php/IJEP/article/view/4742 |
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| author | Abhilash Padhan T. Pradeep Kumar |
| author_facet | Abhilash Padhan T. Pradeep Kumar |
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The present study was carried out during May–July, 2015 at the High-tech seed testing laboratory under the Department of Vegetable Science, Kerala Agriculture University, Trissur, India to differentiate between the normal diploid(2x) watermelon var. Sugar Baby with the yellow seedless triploid(3x) watermelon hybrid var. Swarna (KAU-CL-TETRA-1 × CL-5). In the present study chloroplast count and pollen grain study were undertaken as the morphological markers to carry out the experiment. The true leaf samples collected from 10 numbers of diploid(2x) var. Sugar Baby and 7 numbers of triploid (3x) var. Swarna(KAU-CL-TETRA-1 × CL-5) seedlings of watermelon were taken for the study whereas Binocular compound microscope (Olympus ICON-Tr Freedom) at 100x magnification was used for counting the number of chloroplasts present in the guard cell of stomata. Around 40 numbers of stomatal guard cells were taken into consideration for counting the average chloroplast number for each sample. The pollen grain was studied after staining with red safranin and observed under the compound microscope. The results obtained from the average chloroplast count of 10 samples of diploid (2x) watermelon var. Sugar Baby was 11.07 whereas the average chloroplast count of 7 samples of triploid (3x) watermelon hybrid var. Swarna (KAU-CL-TETRA-1 × CL-5) was 12.11. The pollen grain of triploid (3x) watermelon hybrid var. Swarna (KAU-CL-TETRA-1 × CL-5) was found to be not retained from red safranin stain color being sterile in nature whereas the fertile pollen of diploid (2x) watermelon var. Sugar Baby retained the stain color.
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| format | Article |
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| language | English |
| publishDate | 2022-08-01 |
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| series | International Journal of Economic Plants |
| spelling | doaj-art-a273fc631a0d4f8080ce18ae7946249f2025-08-20T02:37:46ZengPuspa Publishing HouseInternational Journal of Economic Plants2349-47352022-08-019Aug, 3A Comparative Study for Various Morphological Characters between Diploid and Triploid WatermelonAbhilash Padhan0T. Pradeep Kumar1Dept. of Fruit Science, Dr. Y S Parmar University of Hort. and Forestry, Nauni, Solan, Himachal Pradesh (173 230), IndiaDept. of Vegetable Science, Kerala Agriculture University, Vellanikkara, Trissur, Kerala (680 656), India The present study was carried out during May–July, 2015 at the High-tech seed testing laboratory under the Department of Vegetable Science, Kerala Agriculture University, Trissur, India to differentiate between the normal diploid(2x) watermelon var. Sugar Baby with the yellow seedless triploid(3x) watermelon hybrid var. Swarna (KAU-CL-TETRA-1 × CL-5). In the present study chloroplast count and pollen grain study were undertaken as the morphological markers to carry out the experiment. The true leaf samples collected from 10 numbers of diploid(2x) var. Sugar Baby and 7 numbers of triploid (3x) var. Swarna(KAU-CL-TETRA-1 × CL-5) seedlings of watermelon were taken for the study whereas Binocular compound microscope (Olympus ICON-Tr Freedom) at 100x magnification was used for counting the number of chloroplasts present in the guard cell of stomata. Around 40 numbers of stomatal guard cells were taken into consideration for counting the average chloroplast number for each sample. The pollen grain was studied after staining with red safranin and observed under the compound microscope. The results obtained from the average chloroplast count of 10 samples of diploid (2x) watermelon var. Sugar Baby was 11.07 whereas the average chloroplast count of 7 samples of triploid (3x) watermelon hybrid var. Swarna (KAU-CL-TETRA-1 × CL-5) was 12.11. The pollen grain of triploid (3x) watermelon hybrid var. Swarna (KAU-CL-TETRA-1 × CL-5) was found to be not retained from red safranin stain color being sterile in nature whereas the fertile pollen of diploid (2x) watermelon var. Sugar Baby retained the stain color. https://ojs.pphouse.org/index.php/IJEP/article/view/4742Chloroplast, polyploidy, swarna, triploid, watermelon |
| spellingShingle | Abhilash Padhan T. Pradeep Kumar A Comparative Study for Various Morphological Characters between Diploid and Triploid Watermelon International Journal of Economic Plants Chloroplast, polyploidy, swarna, triploid, watermelon |
| title | A Comparative Study for Various Morphological Characters between Diploid and Triploid Watermelon |
| title_full | A Comparative Study for Various Morphological Characters between Diploid and Triploid Watermelon |
| title_fullStr | A Comparative Study for Various Morphological Characters between Diploid and Triploid Watermelon |
| title_full_unstemmed | A Comparative Study for Various Morphological Characters between Diploid and Triploid Watermelon |
| title_short | A Comparative Study for Various Morphological Characters between Diploid and Triploid Watermelon |
| title_sort | comparative study for various morphological characters between diploid and triploid watermelon |
| topic | Chloroplast, polyploidy, swarna, triploid, watermelon |
| url | https://ojs.pphouse.org/index.php/IJEP/article/view/4742 |
| work_keys_str_mv | AT abhilashpadhan acomparativestudyforvariousmorphologicalcharactersbetweendiploidandtriploidwatermelon AT tpradeepkumar acomparativestudyforvariousmorphologicalcharactersbetweendiploidandtriploidwatermelon AT abhilashpadhan comparativestudyforvariousmorphologicalcharactersbetweendiploidandtriploidwatermelon AT tpradeepkumar comparativestudyforvariousmorphologicalcharactersbetweendiploidandtriploidwatermelon |