Assessment of histology's performance compared with PCR in the diagnosis of Helicobacter pylori infection

Aim: Histology is the most widely used test to detect H. pylori. PCR is less used but allows the detection of both infection and antibiotics' resistance. Methods: We conducted a monocentric cross-sectional study, collecting 97 symptomatic patients to assess the diagnostic performance of histolo...

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Main Authors: Manel Moalla, Lassaad Chtourou, Basma Mnif, Slim Charfi, Hend Smaoui, Mon Boudabous, Leila Mnif, Ali Amouri, Hela Gdoura, Adnene Hammami, Tahya Boudawara, Nabil Tahri
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Future Science OA
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Online Access:https://www.tandfonline.com/doi/10.2144/fsoa-2023-0217
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author Manel Moalla
Lassaad Chtourou
Basma Mnif
Slim Charfi
Hend Smaoui
Mon Boudabous
Leila Mnif
Ali Amouri
Hela Gdoura
Adnene Hammami
Tahya Boudawara
Nabil Tahri
author_facet Manel Moalla
Lassaad Chtourou
Basma Mnif
Slim Charfi
Hend Smaoui
Mon Boudabous
Leila Mnif
Ali Amouri
Hela Gdoura
Adnene Hammami
Tahya Boudawara
Nabil Tahri
author_sort Manel Moalla
collection DOAJ
description Aim: Histology is the most widely used test to detect H. pylori. PCR is less used but allows the detection of both infection and antibiotics' resistance. Methods: We conducted a monocentric cross-sectional study, collecting 97 symptomatic patients to assess the diagnostic performance of histology in the detection of H. pylori infection compared with PCR. Results: Sensitivity of histology in comparison with PCR was 81.5% and specificity was 56.3%. A history of anti-H. pylori therapy intake, as well as the density of the bacterium on the gastric sample and the presence of gastric atrophy, were significantly correlated to the PCR's result in terms of H. pylori detection. Conclusion: Thus, histology can be considered as an efficient test compared with PCR in H. pylori detection.
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institution DOAJ
issn 2056-5623
language English
publishDate 2024-12-01
publisher Taylor & Francis Group
record_format Article
series Future Science OA
spelling doaj-art-a271eee4d1d84fcb8ead2262dc4c315d2025-08-20T02:41:39ZengTaylor & Francis GroupFuture Science OA2056-56232024-12-0110110.2144/fsoa-2023-0217Assessment of histology's performance compared with PCR in the diagnosis of Helicobacter pylori infectionManel Moalla0Lassaad Chtourou1Basma Mnif2Slim Charfi3Hend Smaoui4Mon Boudabous5Leila Mnif6Ali Amouri7Hela Gdoura8Adnene Hammami9Tahya Boudawara10Nabil Tahri11Gastroenterology department, Hedi Chaker hospital, Sfax, TunisiaGastroenterology department, Hedi Chaker hospital, Sfax, TunisiaMicrobiology department, Habib Bourguiba hospital, Sfax, TunisiaPathology department, Habib Bourguiba hospital, Sfax, TunisiaGastroenterology department, Hedi Chaker hospital, Sfax, TunisiaGastroenterology department, Hedi Chaker hospital, Sfax, TunisiaGastroenterology department, Hedi Chaker hospital, Sfax, TunisiaGastroenterology department, Hedi Chaker hospital, Sfax, TunisiaGastroenterology department, Hedi Chaker hospital, Sfax, TunisiaMicrobiology department, Habib Bourguiba hospital, Sfax, TunisiaPathology department, Habib Bourguiba hospital, Sfax, TunisiaGastroenterology department, Hedi Chaker hospital, Sfax, TunisiaAim: Histology is the most widely used test to detect H. pylori. PCR is less used but allows the detection of both infection and antibiotics' resistance. Methods: We conducted a monocentric cross-sectional study, collecting 97 symptomatic patients to assess the diagnostic performance of histology in the detection of H. pylori infection compared with PCR. Results: Sensitivity of histology in comparison with PCR was 81.5% and specificity was 56.3%. A history of anti-H. pylori therapy intake, as well as the density of the bacterium on the gastric sample and the presence of gastric atrophy, were significantly correlated to the PCR's result in terms of H. pylori detection. Conclusion: Thus, histology can be considered as an efficient test compared with PCR in H. pylori detection.https://www.tandfonline.com/doi/10.2144/fsoa-2023-0217diagnostic methodsHelicobacter pylorihistologypathologyPCR
spellingShingle Manel Moalla
Lassaad Chtourou
Basma Mnif
Slim Charfi
Hend Smaoui
Mon Boudabous
Leila Mnif
Ali Amouri
Hela Gdoura
Adnene Hammami
Tahya Boudawara
Nabil Tahri
Assessment of histology's performance compared with PCR in the diagnosis of Helicobacter pylori infection
Future Science OA
diagnostic methods
Helicobacter pylori
histology
pathology
PCR
title Assessment of histology's performance compared with PCR in the diagnosis of Helicobacter pylori infection
title_full Assessment of histology's performance compared with PCR in the diagnosis of Helicobacter pylori infection
title_fullStr Assessment of histology's performance compared with PCR in the diagnosis of Helicobacter pylori infection
title_full_unstemmed Assessment of histology's performance compared with PCR in the diagnosis of Helicobacter pylori infection
title_short Assessment of histology's performance compared with PCR in the diagnosis of Helicobacter pylori infection
title_sort assessment of histology s performance compared with pcr in the diagnosis of helicobacter pylori infection
topic diagnostic methods
Helicobacter pylori
histology
pathology
PCR
url https://www.tandfonline.com/doi/10.2144/fsoa-2023-0217
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