miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons.
An array of signals regulating the early stages of postnatal subventricular zone (SVZ) neurogenesis has been identified, but much less is known regarding the molecules controlling late stages. Here, we investigated the function of the activity-dependent and morphogenic microRNA miR-132 on the synapt...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0038174&type=printable |
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| author | Manavendra Pathania Juan Torres-Reveron Lily Yan Tomoki Kimura Tiffany V Lin Valerie Gordon Zhao-Qian Teng Xinyu Zhao Tudor A Fulga David Van Vactor Angélique Bordey |
| author_facet | Manavendra Pathania Juan Torres-Reveron Lily Yan Tomoki Kimura Tiffany V Lin Valerie Gordon Zhao-Qian Teng Xinyu Zhao Tudor A Fulga David Van Vactor Angélique Bordey |
| author_sort | Manavendra Pathania |
| collection | DOAJ |
| description | An array of signals regulating the early stages of postnatal subventricular zone (SVZ) neurogenesis has been identified, but much less is known regarding the molecules controlling late stages. Here, we investigated the function of the activity-dependent and morphogenic microRNA miR-132 on the synaptic integration and survival of olfactory bulb (OB) neurons born in the neonatal SVZ. In situ hybridization revealed that miR-132 expression occurs at the onset of synaptic integration in the OB. Using in vivo electroporation we found that sequestration of miR-132 using a sponge-based strategy led to a reduced dendritic complexity and spine density while overexpression had the opposite effects. These effects were mirrored with respective changes in the frequency of GABAergic and glutamatergic synaptic inputs reflecting altered synaptic integration. In addition, timely directed overexpression of miR-132 at the onset of synaptic integration using an inducible approach led to a significant increase in the survival of newborn neurons. These data suggest that miR-132 forms the basis of a structural plasticity program seen in SVZ-OB postnatal neurogenesis. miR-132 overexpression in transplanted neurons may thus hold promise for enhancing neuronal survival and improving the outcome of transplant therapies. |
| format | Article |
| id | doaj-art-a26b8ddce4cd42a796ec90b57fecf1b5 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-a26b8ddce4cd42a796ec90b57fecf1b52025-08-20T02:30:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3817410.1371/journal.pone.0038174miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons.Manavendra PathaniaJuan Torres-ReveronLily YanTomoki KimuraTiffany V LinValerie GordonZhao-Qian TengXinyu ZhaoTudor A FulgaDavid Van VactorAngélique BordeyAn array of signals regulating the early stages of postnatal subventricular zone (SVZ) neurogenesis has been identified, but much less is known regarding the molecules controlling late stages. Here, we investigated the function of the activity-dependent and morphogenic microRNA miR-132 on the synaptic integration and survival of olfactory bulb (OB) neurons born in the neonatal SVZ. In situ hybridization revealed that miR-132 expression occurs at the onset of synaptic integration in the OB. Using in vivo electroporation we found that sequestration of miR-132 using a sponge-based strategy led to a reduced dendritic complexity and spine density while overexpression had the opposite effects. These effects were mirrored with respective changes in the frequency of GABAergic and glutamatergic synaptic inputs reflecting altered synaptic integration. In addition, timely directed overexpression of miR-132 at the onset of synaptic integration using an inducible approach led to a significant increase in the survival of newborn neurons. These data suggest that miR-132 forms the basis of a structural plasticity program seen in SVZ-OB postnatal neurogenesis. miR-132 overexpression in transplanted neurons may thus hold promise for enhancing neuronal survival and improving the outcome of transplant therapies.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0038174&type=printable |
| spellingShingle | Manavendra Pathania Juan Torres-Reveron Lily Yan Tomoki Kimura Tiffany V Lin Valerie Gordon Zhao-Qian Teng Xinyu Zhao Tudor A Fulga David Van Vactor Angélique Bordey miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons. PLoS ONE |
| title | miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons. |
| title_full | miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons. |
| title_fullStr | miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons. |
| title_full_unstemmed | miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons. |
| title_short | miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons. |
| title_sort | mir 132 enhances dendritic morphogenesis spine density synaptic integration and survival of newborn olfactory bulb neurons |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0038174&type=printable |
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