Proteomic analysis of Down syndrome cerebrospinal fluid compared to late-onset and autosomal dominant Alzheimer´s disease

Abstract Almost all individuals with Down Syndrome (DS) develop Alzheimer’s disease (AD) by mid to late life. However, the degree to which AD in DS shares pathological changes with sporadic late-onset AD (LOAD) and autosomal dominant AD (ADAD) beyond core AD biomarkers such as amyloid-β (Aβ) and tau...

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Main Authors: Laia Montoliu-Gaya, Shijia Bian, Eric B. Dammer, Daniel Alcolea, Mathias Sauer, Mitchell Martá-Ariza, Nicholas J. Ashton, Olivia Belbin, Johannes Fuchs, Caroline M. Watson, Lingyan Ping, Duc M. Duong, Johanna Nilsson, Isabel Barroeta, Juan Lantero-Rodriguez, Laura Videla, Bessy Benejam, Blaine R. Roberts, Kaj Blennow, Nicholas T. Seyfried, Allan I. Levey, María Carmona-Iragui, Johan Gobom, Alberto Lleó, Thomas Wisniewski, Henrik Zetterberg, Juan Fortea, Erik C. B. Johnson
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-61054-z
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author Laia Montoliu-Gaya
Shijia Bian
Eric B. Dammer
Daniel Alcolea
Mathias Sauer
Mitchell Martá-Ariza
Nicholas J. Ashton
Olivia Belbin
Johannes Fuchs
Caroline M. Watson
Lingyan Ping
Duc M. Duong
Johanna Nilsson
Isabel Barroeta
Juan Lantero-Rodriguez
Laura Videla
Bessy Benejam
Blaine R. Roberts
Kaj Blennow
Nicholas T. Seyfried
Allan I. Levey
María Carmona-Iragui
Johan Gobom
Alberto Lleó
Thomas Wisniewski
Henrik Zetterberg
Juan Fortea
Erik C. B. Johnson
author_facet Laia Montoliu-Gaya
Shijia Bian
Eric B. Dammer
Daniel Alcolea
Mathias Sauer
Mitchell Martá-Ariza
Nicholas J. Ashton
Olivia Belbin
Johannes Fuchs
Caroline M. Watson
Lingyan Ping
Duc M. Duong
Johanna Nilsson
Isabel Barroeta
Juan Lantero-Rodriguez
Laura Videla
Bessy Benejam
Blaine R. Roberts
Kaj Blennow
Nicholas T. Seyfried
Allan I. Levey
María Carmona-Iragui
Johan Gobom
Alberto Lleó
Thomas Wisniewski
Henrik Zetterberg
Juan Fortea
Erik C. B. Johnson
author_sort Laia Montoliu-Gaya
collection DOAJ
description Abstract Almost all individuals with Down Syndrome (DS) develop Alzheimer’s disease (AD) by mid to late life. However, the degree to which AD in DS shares pathological changes with sporadic late-onset AD (LOAD) and autosomal dominant AD (ADAD) beyond core AD biomarkers such as amyloid-β (Aβ) and tau is unknown. Here, we used proteomics of cerebrospinal fluid from individuals with DS (n = 229) in the Down Alzheimer Barcelona Neuroimaging Initiative (DABNI) cohort to assess the evolution of AD pathophysiology from asymptomatic to dementia stages and compared the proteomic biomarker changes in DS to those observed in LOAD and ADAD. Although many proteomic alterations were shared across DS, LOAD, and ADAD, DS demonstrated more severe changes in immune-related proteins, extracellular matrix pathways, and plasma proteins likely related to blood-brain barrier dysfunction compared to LOAD. These changes were present in young adults with DS prior to the onset of Aβ or tau pathology, suggesting they are associated with trisomy 21 and may serve as risk factors for DSAD. DSAD showed an earlier increase in markers of axonal and white matter pathology and earlier changes in markers potentially associated with cerebral amyloid angiopathy compared to ADAD. The unique features of DSAD may have important implications for treatment strategies in this population.
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spelling doaj-art-a260fac5cb9f42f4a0107d1767bb28ef2025-08-20T04:02:57ZengNature PortfolioNature Communications2041-17232025-07-0116112010.1038/s41467-025-61054-zProteomic analysis of Down syndrome cerebrospinal fluid compared to late-onset and autosomal dominant Alzheimer´s diseaseLaia Montoliu-Gaya0Shijia Bian1Eric B. Dammer2Daniel Alcolea3Mathias Sauer4Mitchell Martá-Ariza5Nicholas J. Ashton6Olivia Belbin7Johannes Fuchs8Caroline M. Watson9Lingyan Ping10Duc M. Duong11Johanna Nilsson12Isabel Barroeta13Juan Lantero-Rodriguez14Laura Videla15Bessy Benejam16Blaine R. Roberts17Kaj Blennow18Nicholas T. Seyfried19Allan I. Levey20María Carmona-Iragui21Johan Gobom22Alberto Lleó23Thomas Wisniewski24Henrik Zetterberg25Juan Fortea26Erik C. B. Johnson27Department of Psychiatry and Neurochemistry, Institute of Neuroscience & Physiology, The Sahlgrenska Academy at the University of GothenburgDepartment of Biostatistics and Bioinformatics, Rollins School of Public Health, Emory UniversityDepartment of Biochemistry, Emory University School of MedicineSant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Universitat Autònoma de BarcelonaDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience & Physiology, The Sahlgrenska Academy at the University of GothenburgDepartment of Neurology, NYU Grossman School of MedicineDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience & Physiology, The Sahlgrenska Academy at the University of GothenburgSant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Universitat Autònoma de BarcelonaProteomics Core Facility at Sahlgrenska Academy, University of GothenburgGoizueta Alzheimer’s Disease Research Center, Emory University School of MedicineDepartment of Biochemistry, Emory University School of MedicineDepartment of Biochemistry, Emory University School of MedicineDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience & Physiology, The Sahlgrenska Academy at the University of GothenburgSant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Universitat Autònoma de BarcelonaDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience & Physiology, The Sahlgrenska Academy at the University of GothenburgSant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Universitat Autònoma de BarcelonaSant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Universitat Autònoma de BarcelonaDepartment of Biochemistry, Emory University School of MedicineDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience & Physiology, The Sahlgrenska Academy at the University of GothenburgDepartment of Biochemistry, Emory University School of MedicineGoizueta Alzheimer’s Disease Research Center, Emory University School of MedicineSant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Universitat Autònoma de BarcelonaDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience & Physiology, The Sahlgrenska Academy at the University of GothenburgSant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Universitat Autònoma de BarcelonaDepartment of Neurology, NYU Grossman School of MedicineDepartment of Psychiatry and Neurochemistry, Institute of Neuroscience & Physiology, The Sahlgrenska Academy at the University of GothenburgSant Pau Memory Unit, Department of Neurology, Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), Universitat Autònoma de BarcelonaGoizueta Alzheimer’s Disease Research Center, Emory University School of MedicineAbstract Almost all individuals with Down Syndrome (DS) develop Alzheimer’s disease (AD) by mid to late life. However, the degree to which AD in DS shares pathological changes with sporadic late-onset AD (LOAD) and autosomal dominant AD (ADAD) beyond core AD biomarkers such as amyloid-β (Aβ) and tau is unknown. Here, we used proteomics of cerebrospinal fluid from individuals with DS (n = 229) in the Down Alzheimer Barcelona Neuroimaging Initiative (DABNI) cohort to assess the evolution of AD pathophysiology from asymptomatic to dementia stages and compared the proteomic biomarker changes in DS to those observed in LOAD and ADAD. Although many proteomic alterations were shared across DS, LOAD, and ADAD, DS demonstrated more severe changes in immune-related proteins, extracellular matrix pathways, and plasma proteins likely related to blood-brain barrier dysfunction compared to LOAD. These changes were present in young adults with DS prior to the onset of Aβ or tau pathology, suggesting they are associated with trisomy 21 and may serve as risk factors for DSAD. DSAD showed an earlier increase in markers of axonal and white matter pathology and earlier changes in markers potentially associated with cerebral amyloid angiopathy compared to ADAD. The unique features of DSAD may have important implications for treatment strategies in this population.https://doi.org/10.1038/s41467-025-61054-z
spellingShingle Laia Montoliu-Gaya
Shijia Bian
Eric B. Dammer
Daniel Alcolea
Mathias Sauer
Mitchell Martá-Ariza
Nicholas J. Ashton
Olivia Belbin
Johannes Fuchs
Caroline M. Watson
Lingyan Ping
Duc M. Duong
Johanna Nilsson
Isabel Barroeta
Juan Lantero-Rodriguez
Laura Videla
Bessy Benejam
Blaine R. Roberts
Kaj Blennow
Nicholas T. Seyfried
Allan I. Levey
María Carmona-Iragui
Johan Gobom
Alberto Lleó
Thomas Wisniewski
Henrik Zetterberg
Juan Fortea
Erik C. B. Johnson
Proteomic analysis of Down syndrome cerebrospinal fluid compared to late-onset and autosomal dominant Alzheimer´s disease
Nature Communications
title Proteomic analysis of Down syndrome cerebrospinal fluid compared to late-onset and autosomal dominant Alzheimer´s disease
title_full Proteomic analysis of Down syndrome cerebrospinal fluid compared to late-onset and autosomal dominant Alzheimer´s disease
title_fullStr Proteomic analysis of Down syndrome cerebrospinal fluid compared to late-onset and autosomal dominant Alzheimer´s disease
title_full_unstemmed Proteomic analysis of Down syndrome cerebrospinal fluid compared to late-onset and autosomal dominant Alzheimer´s disease
title_short Proteomic analysis of Down syndrome cerebrospinal fluid compared to late-onset and autosomal dominant Alzheimer´s disease
title_sort proteomic analysis of down syndrome cerebrospinal fluid compared to late onset and autosomal dominant alzheimer´s disease
url https://doi.org/10.1038/s41467-025-61054-z
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