Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity.
We describe three recombinant engineered antibodies against three recombinant epitope tags, constructed with divalent binding arms to recognize divalent epitopes and so achieve high affinity and specificity. In two versions, an epitope is inserted in tandem into a protein of interest, and a homodime...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150125&type=printable |
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| _version_ | 1850188918608101376 |
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| author | Hongyu Zhao Ao Shen Yang K Xiang David P Corey |
| author_facet | Hongyu Zhao Ao Shen Yang K Xiang David P Corey |
| author_sort | Hongyu Zhao |
| collection | DOAJ |
| description | We describe three recombinant engineered antibodies against three recombinant epitope tags, constructed with divalent binding arms to recognize divalent epitopes and so achieve high affinity and specificity. In two versions, an epitope is inserted in tandem into a protein of interest, and a homodimeric antibody is constructed by fusing a high-affinity epitope-binding domain to a human or mouse Fc domain. In a third, a heterodimeric antibody is constructed by fusing two different epitope-binding domains which target two different binding sites in GFP, to polarized Fc fragments. These antibody/epitope pairs have affinities in the low picomolar range and are useful tools for many antibody-based applications. |
| format | Article |
| id | doaj-art-a25f87d79c794d2ea4a40de0730b7ba8 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-a25f87d79c794d2ea4a40de0730b7ba82025-08-20T02:15:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015012510.1371/journal.pone.0150125Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity.Hongyu ZhaoAo ShenYang K XiangDavid P CoreyWe describe three recombinant engineered antibodies against three recombinant epitope tags, constructed with divalent binding arms to recognize divalent epitopes and so achieve high affinity and specificity. In two versions, an epitope is inserted in tandem into a protein of interest, and a homodimeric antibody is constructed by fusing a high-affinity epitope-binding domain to a human or mouse Fc domain. In a third, a heterodimeric antibody is constructed by fusing two different epitope-binding domains which target two different binding sites in GFP, to polarized Fc fragments. These antibody/epitope pairs have affinities in the low picomolar range and are useful tools for many antibody-based applications.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150125&type=printable |
| spellingShingle | Hongyu Zhao Ao Shen Yang K Xiang David P Corey Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity. PLoS ONE |
| title | Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity. |
| title_full | Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity. |
| title_fullStr | Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity. |
| title_full_unstemmed | Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity. |
| title_short | Three Recombinant Engineered Antibodies against Recombinant Tags with High Affinity and Specificity. |
| title_sort | three recombinant engineered antibodies against recombinant tags with high affinity and specificity |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0150125&type=printable |
| work_keys_str_mv | AT hongyuzhao threerecombinantengineeredantibodiesagainstrecombinanttagswithhighaffinityandspecificity AT aoshen threerecombinantengineeredantibodiesagainstrecombinanttagswithhighaffinityandspecificity AT yangkxiang threerecombinantengineeredantibodiesagainstrecombinanttagswithhighaffinityandspecificity AT davidpcorey threerecombinantengineeredantibodiesagainstrecombinanttagswithhighaffinityandspecificity |