Detection of Salmonella spp. Related Co-Infections Among Children with Diarrheal Diseases in Guangzhou, China

Qiongdan Mai,* Yasha Luo,* Ruoting Ye, Yongyao Jiang, Yanting Qin, Junfei Guo, Weiming Lai, Yongbing Wu, Mingyong Luo Department of Clinical Laboratory, Guangdong Women and Children Hospital, Guangzhou, People’s Republic of China*These authors contributed equally to this work...

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Main Authors: Mai Q, Luo Y, Ye R, Jiang Y, Qin Y, Guo J, Lai W, Wu Y, Luo M
Format: Article
Language:English
Published: Dove Medical Press 2025-04-01
Series:Infection and Drug Resistance
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Online Access:https://www.dovepress.com/detection-of-salmonella-spp-related-co-infections-among-children-with--peer-reviewed-fulltext-article-IDR
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author Mai Q
Luo Y
Ye R
Jiang Y
Qin Y
Guo J
Lai W
Wu Y
Luo M
author_facet Mai Q
Luo Y
Ye R
Jiang Y
Qin Y
Guo J
Lai W
Wu Y
Luo M
author_sort Mai Q
collection DOAJ
description Qiongdan Mai,&ast; Yasha Luo,&ast; Ruoting Ye, Yongyao Jiang, Yanting Qin, Junfei Guo, Weiming Lai, Yongbing Wu, Mingyong Luo Department of Clinical Laboratory, Guangdong Women and Children Hospital, Guangzhou, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Mingyong Luo, Department of Clinical Laboratory, Guangdong Women and Children Hospital, NO. 521 Xingnan Road, Panyu District, Guangzhou, People’s Republic of China, Tel +86-15920356428, Email luo-my@163.comBackground: Diarrheal diseases caused by gastrointestinal pathogens contribute to the high morbidity and mortality in children worldwide. Salmonella infection is one of the leading causes of diarrhea, especially in children under 5 years of age. This study aimed to assess the prevalence of Salmonella infection and its co-infection patterns in relation to clinical symptoms.Methods: A total of 430 stool samples of children with diarrheal diseases were collected from Guangdong Women and Children’s Hospital during January 2022 to December 2023 and used for detection. BioFire FilmArray Gastrointestinal (GI) Panel is an efficient and sensitive method used to assess infections caused by enteric pathogens simultaneously based on multiplex polymerase chain reaction (PCR) technologies.Results: Salmonella spp. was classified as the predominant pathogen in all stool specimens, with an overall positivity rate of 36.74% (158/430), of which 35.44% (56/158) were identified as Salmonella single infection. For Salmonella-related bacterial co-infection pattern, Salmonella and enteropathogenic Escherichia coli combinations accounted for 12.66% (20/158) of Salmonella-related bacterial co-infection patterns, and Salmonella plus Clostridium difficile was found in 8.86% (14/158). For Salmonella-viral co-infection pattern, the most prevalent combination was Salmonella and adenovirus (6.33%, 10/158). Notably, the proportion of mucus stools recorded in Salmonella plus C. difficile infections was statistically higher than that in single Salmonella infections (P < 0.05).Conclusion: This study provides a comprehensive understanding of the nature of Salmonella spp. co-infections in diarrheal diseases, and the possibility of clinical symptoms and enhanced treatments.Keywords: enteric pathogens, gastrointestinal infection, diarrhea, FilmArray
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series Infection and Drug Resistance
spelling doaj-art-a25e5241a11e4795ba55ca2edde1eb8a2025-08-20T02:56:01ZengDove Medical PressInfection and Drug Resistance1178-69732025-04-01Volume 1818951903102164Detection of Salmonella spp. Related Co-Infections Among Children with Diarrheal Diseases in Guangzhou, ChinaMai QLuo YYe RJiang YQin YGuo JLai WWu YLuo MQiongdan Mai,&ast; Yasha Luo,&ast; Ruoting Ye, Yongyao Jiang, Yanting Qin, Junfei Guo, Weiming Lai, Yongbing Wu, Mingyong Luo Department of Clinical Laboratory, Guangdong Women and Children Hospital, Guangzhou, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Mingyong Luo, Department of Clinical Laboratory, Guangdong Women and Children Hospital, NO. 521 Xingnan Road, Panyu District, Guangzhou, People’s Republic of China, Tel +86-15920356428, Email luo-my@163.comBackground: Diarrheal diseases caused by gastrointestinal pathogens contribute to the high morbidity and mortality in children worldwide. Salmonella infection is one of the leading causes of diarrhea, especially in children under 5 years of age. This study aimed to assess the prevalence of Salmonella infection and its co-infection patterns in relation to clinical symptoms.Methods: A total of 430 stool samples of children with diarrheal diseases were collected from Guangdong Women and Children’s Hospital during January 2022 to December 2023 and used for detection. BioFire FilmArray Gastrointestinal (GI) Panel is an efficient and sensitive method used to assess infections caused by enteric pathogens simultaneously based on multiplex polymerase chain reaction (PCR) technologies.Results: Salmonella spp. was classified as the predominant pathogen in all stool specimens, with an overall positivity rate of 36.74% (158/430), of which 35.44% (56/158) were identified as Salmonella single infection. For Salmonella-related bacterial co-infection pattern, Salmonella and enteropathogenic Escherichia coli combinations accounted for 12.66% (20/158) of Salmonella-related bacterial co-infection patterns, and Salmonella plus Clostridium difficile was found in 8.86% (14/158). For Salmonella-viral co-infection pattern, the most prevalent combination was Salmonella and adenovirus (6.33%, 10/158). Notably, the proportion of mucus stools recorded in Salmonella plus C. difficile infections was statistically higher than that in single Salmonella infections (P < 0.05).Conclusion: This study provides a comprehensive understanding of the nature of Salmonella spp. co-infections in diarrheal diseases, and the possibility of clinical symptoms and enhanced treatments.Keywords: enteric pathogens, gastrointestinal infection, diarrhea, FilmArrayhttps://www.dovepress.com/detection-of-salmonella-spp-related-co-infections-among-children-with--peer-reviewed-fulltext-article-IDRenteric pathogensgastrointestinal infectiondiarrheafilmarray.
spellingShingle Mai Q
Luo Y
Ye R
Jiang Y
Qin Y
Guo J
Lai W
Wu Y
Luo M
Detection of Salmonella spp. Related Co-Infections Among Children with Diarrheal Diseases in Guangzhou, China
Infection and Drug Resistance
enteric pathogens
gastrointestinal infection
diarrhea
filmarray.
title Detection of Salmonella spp. Related Co-Infections Among Children with Diarrheal Diseases in Guangzhou, China
title_full Detection of Salmonella spp. Related Co-Infections Among Children with Diarrheal Diseases in Guangzhou, China
title_fullStr Detection of Salmonella spp. Related Co-Infections Among Children with Diarrheal Diseases in Guangzhou, China
title_full_unstemmed Detection of Salmonella spp. Related Co-Infections Among Children with Diarrheal Diseases in Guangzhou, China
title_short Detection of Salmonella spp. Related Co-Infections Among Children with Diarrheal Diseases in Guangzhou, China
title_sort detection of salmonella spp related co infections among children with diarrheal diseases in guangzhou china
topic enteric pathogens
gastrointestinal infection
diarrhea
filmarray.
url https://www.dovepress.com/detection-of-salmonella-spp-related-co-infections-among-children-with--peer-reviewed-fulltext-article-IDR
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