Effect of mild gestational diabetes mellitus on histological, ultrastructural, and quantitative morphometric alterations of rat fetal liver
Objective(s): Gestational diabetes mellitus (GDM), one of the most common metabolic disorders in pregnancy, impacts maternal and fetal health. This study was designed to assess the effects of mild GDM on the histology, ultrastructure, and morphometry of fetal liver tissue.Materials and Methods: In t...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2024-11-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_24699_42be06c086da47a7ab0869a4849b1fda.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849412437992800256 |
|---|---|
| author | Sahar Ardalan Khales Asghar Mafinezhad Masoud Golalipour Gholamreza Roshandel Soraya Ghafari Abdolhalim Rajabi Mohammad Jafar Golalipour |
| author_facet | Sahar Ardalan Khales Asghar Mafinezhad Masoud Golalipour Gholamreza Roshandel Soraya Ghafari Abdolhalim Rajabi Mohammad Jafar Golalipour |
| author_sort | Sahar Ardalan Khales |
| collection | DOAJ |
| description | Objective(s): Gestational diabetes mellitus (GDM), one of the most common metabolic disorders in pregnancy, impacts maternal and fetal health. This study was designed to assess the effects of mild GDM on the histology, ultrastructure, and morphometry of fetal liver tissue.Materials and Methods: In this experimental study, twenty pregnant rats were randomly allocated into control and streptozotocin (STZ)-induced diabetic groups. Mild hyperglycemia was induced by intraperitoneal injection of STZ (40 mg/kg/bw) on the 5th day of gestation. At day 19 of gestation, fetal livers were separated and subjected to histological, transmission electron microscopic, and quantitative morphometric examinations.Results: In the GDM group, PAS staining was positive, revealing scattered eosinophilic inclusions in some hepatocytes. Masson trichrome staining was also positive and showed some fibrous tissue as fine fibers in the portal spaces that extended to the central vein. Reticulin staining in the GDM group was focally positive in the areas of fibrosis and the portal spaces. Ultrastructural examination showed pyknotic nuclei, karyolysis, degranulation and vesiculation of the rough endoplasmic reticulum, and degeneration of mitochondria in the GDM group. The morphometric examination demonstrated that the mean area of hepatocytes was significantly lower in the GDM group than in the control group (P<0.05). Moreover, the mean diameter of the central vein and the density of megakaryocytes were significantly higher in the GDM group than in the control group (P<0.05). Conclusion: Uncontrolled mild GDM induced the histological, ultrastructural and morphometric alterations in the fetal liver. |
| format | Article |
| id | doaj-art-a2567e7a93cd4ed9abcb18dbb36098c3 |
| institution | Kabale University |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-a2567e7a93cd4ed9abcb18dbb36098c32025-08-20T03:34:26ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742024-11-0127111405140910.22038/ijbms.2024.78920.1706924699Effect of mild gestational diabetes mellitus on histological, ultrastructural, and quantitative morphometric alterations of rat fetal liverSahar Ardalan Khales0Asghar Mafinezhad1Masoud Golalipour2Gholamreza Roshandel3Soraya Ghafari4Abdolhalim Rajabi5Mohammad Jafar Golalipour6Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, IranPathology Department of Shahid Kamyab Hospital, Mashhad University of Medical Sciences, Mashhad, IranMedical Cellular and Molecular Research Centre, Golestan University of Medical Sciences, Gorgan, IranGolestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, IranGorgan Congenital Malformations Research Center, Golestan University of Medical Sciences, Gorgan, IranEnvironmental Health Research Center, Department of Biostatistics and Epidemiology, Faculty of Health, Golestan University of Medical Sciences, Gorgan, IranGorgan Congenital Malformations Research Center, Golestan University of Medical Sciences, Gorgan, IranObjective(s): Gestational diabetes mellitus (GDM), one of the most common metabolic disorders in pregnancy, impacts maternal and fetal health. This study was designed to assess the effects of mild GDM on the histology, ultrastructure, and morphometry of fetal liver tissue.Materials and Methods: In this experimental study, twenty pregnant rats were randomly allocated into control and streptozotocin (STZ)-induced diabetic groups. Mild hyperglycemia was induced by intraperitoneal injection of STZ (40 mg/kg/bw) on the 5th day of gestation. At day 19 of gestation, fetal livers were separated and subjected to histological, transmission electron microscopic, and quantitative morphometric examinations.Results: In the GDM group, PAS staining was positive, revealing scattered eosinophilic inclusions in some hepatocytes. Masson trichrome staining was also positive and showed some fibrous tissue as fine fibers in the portal spaces that extended to the central vein. Reticulin staining in the GDM group was focally positive in the areas of fibrosis and the portal spaces. Ultrastructural examination showed pyknotic nuclei, karyolysis, degranulation and vesiculation of the rough endoplasmic reticulum, and degeneration of mitochondria in the GDM group. The morphometric examination demonstrated that the mean area of hepatocytes was significantly lower in the GDM group than in the control group (P<0.05). Moreover, the mean diameter of the central vein and the density of megakaryocytes were significantly higher in the GDM group than in the control group (P<0.05). Conclusion: Uncontrolled mild GDM induced the histological, ultrastructural and morphometric alterations in the fetal liver.https://ijbms.mums.ac.ir/article_24699_42be06c086da47a7ab0869a4849b1fda.pdffetusgestational diabetes mellitushistologylivermorphometryratultrastructure |
| spellingShingle | Sahar Ardalan Khales Asghar Mafinezhad Masoud Golalipour Gholamreza Roshandel Soraya Ghafari Abdolhalim Rajabi Mohammad Jafar Golalipour Effect of mild gestational diabetes mellitus on histological, ultrastructural, and quantitative morphometric alterations of rat fetal liver Iranian Journal of Basic Medical Sciences fetus gestational diabetes mellitus histology liver morphometry rat ultrastructure |
| title | Effect of mild gestational diabetes mellitus on histological, ultrastructural, and quantitative morphometric alterations of rat fetal liver |
| title_full | Effect of mild gestational diabetes mellitus on histological, ultrastructural, and quantitative morphometric alterations of rat fetal liver |
| title_fullStr | Effect of mild gestational diabetes mellitus on histological, ultrastructural, and quantitative morphometric alterations of rat fetal liver |
| title_full_unstemmed | Effect of mild gestational diabetes mellitus on histological, ultrastructural, and quantitative morphometric alterations of rat fetal liver |
| title_short | Effect of mild gestational diabetes mellitus on histological, ultrastructural, and quantitative morphometric alterations of rat fetal liver |
| title_sort | effect of mild gestational diabetes mellitus on histological ultrastructural and quantitative morphometric alterations of rat fetal liver |
| topic | fetus gestational diabetes mellitus histology liver morphometry rat ultrastructure |
| url | https://ijbms.mums.ac.ir/article_24699_42be06c086da47a7ab0869a4849b1fda.pdf |
| work_keys_str_mv | AT saharardalankhales effectofmildgestationaldiabetesmellitusonhistologicalultrastructuralandquantitativemorphometricalterationsofratfetalliver AT asgharmafinezhad effectofmildgestationaldiabetesmellitusonhistologicalultrastructuralandquantitativemorphometricalterationsofratfetalliver AT masoudgolalipour effectofmildgestationaldiabetesmellitusonhistologicalultrastructuralandquantitativemorphometricalterationsofratfetalliver AT gholamrezaroshandel effectofmildgestationaldiabetesmellitusonhistologicalultrastructuralandquantitativemorphometricalterationsofratfetalliver AT sorayaghafari effectofmildgestationaldiabetesmellitusonhistologicalultrastructuralandquantitativemorphometricalterationsofratfetalliver AT abdolhalimrajabi effectofmildgestationaldiabetesmellitusonhistologicalultrastructuralandquantitativemorphometricalterationsofratfetalliver AT mohammadjafargolalipour effectofmildgestationaldiabetesmellitusonhistologicalultrastructuralandquantitativemorphometricalterationsofratfetalliver |