Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging
Abstract Rapid and timely selection of appropriate antibiotics minimizes treatment delays, enhances patient care, and improves infection control. The antibiotic disk diffusion method (Kirby–Bauer test) is the most widely used growth-based technique for assessing bacterial susceptibility due to its s...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | Journal of Biological Engineering |
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| Online Access: | https://doi.org/10.1186/s13036-025-00542-8 |
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| author | Donghyeok Kim Seongjoon Moon Jongseo Lee Kyoungman Cho Changhan Lee Jonghee Yoon |
| author_facet | Donghyeok Kim Seongjoon Moon Jongseo Lee Kyoungman Cho Changhan Lee Jonghee Yoon |
| author_sort | Donghyeok Kim |
| collection | DOAJ |
| description | Abstract Rapid and timely selection of appropriate antibiotics minimizes treatment delays, enhances patient care, and improves infection control. The antibiotic disk diffusion method (Kirby–Bauer test) is the most widely used growth-based technique for assessing bacterial susceptibility due to its simplicity and reliability. However, conventional growth-based methods typically require over 12 h of incubation for visible inspection, making them unsuitable for situations requiring urgent treatment. In this study, we developed a novel laser speckle imaging (LSI) system that measures light scattering properties in a medium, along with an advanced image processing method for the quantitative assessment of antimicrobial effects based on bacterial activity. The LSI system utilizes an optical diffuser with controlled rotations to generate multiple independent speckle illumination patterns. The image processing algorithm analyzes correlation contrast in time-series laser speckle images, enabling more precise bacterial activity detection compared to conventional LSI techniques. The proposed method successfully detected effective antibiotics within 3h for both Gram-negative and Gram-positive bacteria, a capability not achievable using traditional bacterial growth-based antimicrobial susceptibility tests. This approach has the potential to serve as a versatile, rapid, and clinically viable tool for identifying effective antibiotics in patients with bacterial infections, significantly improving diagnostic efficiency in clinical settings. |
| format | Article |
| id | doaj-art-a245671bbcb94c989786e7aabd7ba6f8 |
| institution | Kabale University |
| issn | 1754-1611 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj-art-a245671bbcb94c989786e7aabd7ba6f82025-08-20T03:42:53ZengBMCJournal of Biological Engineering1754-16112025-07-0119111110.1186/s13036-025-00542-8Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imagingDonghyeok Kim0Seongjoon Moon1Jongseo Lee2Kyoungman Cho3Changhan Lee4Jonghee Yoon5Department of Energy Systems Research, Ajou UniversityDepartment of Biological Sciences, Ajou UniversityDepartment of Energy Systems Research, Ajou UniversityThe Wave Talk., IncDepartment of Biological Sciences, Ajou UniversityDepartment of Energy Systems Research, Ajou UniversityAbstract Rapid and timely selection of appropriate antibiotics minimizes treatment delays, enhances patient care, and improves infection control. The antibiotic disk diffusion method (Kirby–Bauer test) is the most widely used growth-based technique for assessing bacterial susceptibility due to its simplicity and reliability. However, conventional growth-based methods typically require over 12 h of incubation for visible inspection, making them unsuitable for situations requiring urgent treatment. In this study, we developed a novel laser speckle imaging (LSI) system that measures light scattering properties in a medium, along with an advanced image processing method for the quantitative assessment of antimicrobial effects based on bacterial activity. The LSI system utilizes an optical diffuser with controlled rotations to generate multiple independent speckle illumination patterns. The image processing algorithm analyzes correlation contrast in time-series laser speckle images, enabling more precise bacterial activity detection compared to conventional LSI techniques. The proposed method successfully detected effective antibiotics within 3h for both Gram-negative and Gram-positive bacteria, a capability not achievable using traditional bacterial growth-based antimicrobial susceptibility tests. This approach has the potential to serve as a versatile, rapid, and clinically viable tool for identifying effective antibiotics in patients with bacterial infections, significantly improving diagnostic efficiency in clinical settings.https://doi.org/10.1186/s13036-025-00542-8Laser speckle imagingRandom speckleAntibioticsInhibition zoneDiffusion disk method |
| spellingShingle | Donghyeok Kim Seongjoon Moon Jongseo Lee Kyoungman Cho Changhan Lee Jonghee Yoon Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging Journal of Biological Engineering Laser speckle imaging Random speckle Antibiotics Inhibition zone Diffusion disk method |
| title | Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging |
| title_full | Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging |
| title_fullStr | Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging |
| title_full_unstemmed | Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging |
| title_short | Light scattering-based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging |
| title_sort | light scattering based screening method for rapid evaluating antibiotic effects on bacteria using laser speckle imaging |
| topic | Laser speckle imaging Random speckle Antibiotics Inhibition zone Diffusion disk method |
| url | https://doi.org/10.1186/s13036-025-00542-8 |
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