The potential relationship between EasyNAT system Tt values and Cobas z480 Ct values in the detection of SARS-Cov-2
Objectives: This study aimed to evaluate the potential relationship between the time threshold (Tt) values of a commercial EasyNAT system, which is based on cross priming amplification (CPA) technology, and the cycle threshold (Ct) values of the Cobas z480 analyzer, which is based on a real-time flu...
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Elsevier
2024-11-01
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| Series: | Practical Laboratory Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551724000775 |
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| author | Lu-Qing Zheng Qing-Yong Wang |
| author_facet | Lu-Qing Zheng Qing-Yong Wang |
| author_sort | Lu-Qing Zheng |
| collection | DOAJ |
| description | Objectives: This study aimed to evaluate the potential relationship between the time threshold (Tt) values of a commercial EasyNAT system, which is based on cross priming amplification (CPA) technology, and the cycle threshold (Ct) values of the Cobas z480 analyzer, which is based on a real-time fluorescence polymerase chain reaction (PCR) method, in the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) from oropharyngeal swabs. Design and Methods: Data were retrospectively collected from a clinical laboratory between December 4, 2022 and July 1, 2024. Results: A total of 277 EasyNAT-positive samples (Tt values from 3.83 to 29.5) were simultaneously investigated using the Cobas z480 analyzer (Ct values from 10.74 to 38.78). The concordance rate between the two systems was 100 %. Among the positive samples, the mean and maximum PCR Ct values of O and N genes increased in line with increasing Tt values of the left and right amplification areas of the EasyNAT system. The maximum Ct values of the O or N gene determined by the Cobas z480 analyzer were no more than 29.52 when the Tt values of the left or right amplification areas of the UC0116 analyzer were no more than 6. Conclusions: The safe, simple, fast, accurate, and automatic EasyNAT system used in conjunction with a PCR system might be a better choice for the detection of SARS-CoV-2 in hospitals, especially in settings without sophisticated PCR facilities. The Tt value (≤6) of the EasyNAT system can be a reference index for estimating the maximum Ct value (29.52) in SARS-CoV-2-positive samples. |
| format | Article |
| id | doaj-art-a2402ca61bee44caba22fa9827541984 |
| institution | OA Journals |
| issn | 2352-5517 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
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| series | Practical Laboratory Medicine |
| spelling | doaj-art-a2402ca61bee44caba22fa98275419842025-08-20T01:57:56ZengElsevierPractical Laboratory Medicine2352-55172024-11-0142e0043110.1016/j.plabm.2024.e00431The potential relationship between EasyNAT system Tt values and Cobas z480 Ct values in the detection of SARS-Cov-2Lu-Qing Zheng0Qing-Yong Wang1Department of Clinical Laboratory, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, ChinaCorresponding author. Department of Clinical Laboratory, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China.; Department of Clinical Laboratory, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, ChinaObjectives: This study aimed to evaluate the potential relationship between the time threshold (Tt) values of a commercial EasyNAT system, which is based on cross priming amplification (CPA) technology, and the cycle threshold (Ct) values of the Cobas z480 analyzer, which is based on a real-time fluorescence polymerase chain reaction (PCR) method, in the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) from oropharyngeal swabs. Design and Methods: Data were retrospectively collected from a clinical laboratory between December 4, 2022 and July 1, 2024. Results: A total of 277 EasyNAT-positive samples (Tt values from 3.83 to 29.5) were simultaneously investigated using the Cobas z480 analyzer (Ct values from 10.74 to 38.78). The concordance rate between the two systems was 100 %. Among the positive samples, the mean and maximum PCR Ct values of O and N genes increased in line with increasing Tt values of the left and right amplification areas of the EasyNAT system. The maximum Ct values of the O or N gene determined by the Cobas z480 analyzer were no more than 29.52 when the Tt values of the left or right amplification areas of the UC0116 analyzer were no more than 6. Conclusions: The safe, simple, fast, accurate, and automatic EasyNAT system used in conjunction with a PCR system might be a better choice for the detection of SARS-CoV-2 in hospitals, especially in settings without sophisticated PCR facilities. The Tt value (≤6) of the EasyNAT system can be a reference index for estimating the maximum Ct value (29.52) in SARS-CoV-2-positive samples.http://www.sciencedirect.com/science/article/pii/S2352551724000775EasyNAT systemCobas z480Time thresholdCycle thresholdSARS-CoV-2 |
| spellingShingle | Lu-Qing Zheng Qing-Yong Wang The potential relationship between EasyNAT system Tt values and Cobas z480 Ct values in the detection of SARS-Cov-2 Practical Laboratory Medicine EasyNAT system Cobas z480 Time threshold Cycle threshold SARS-CoV-2 |
| title | The potential relationship between EasyNAT system Tt values and Cobas z480 Ct values in the detection of SARS-Cov-2 |
| title_full | The potential relationship between EasyNAT system Tt values and Cobas z480 Ct values in the detection of SARS-Cov-2 |
| title_fullStr | The potential relationship between EasyNAT system Tt values and Cobas z480 Ct values in the detection of SARS-Cov-2 |
| title_full_unstemmed | The potential relationship between EasyNAT system Tt values and Cobas z480 Ct values in the detection of SARS-Cov-2 |
| title_short | The potential relationship between EasyNAT system Tt values and Cobas z480 Ct values in the detection of SARS-Cov-2 |
| title_sort | potential relationship between easynat system tt values and cobas z480 ct values in the detection of sars cov 2 |
| topic | EasyNAT system Cobas z480 Time threshold Cycle threshold SARS-CoV-2 |
| url | http://www.sciencedirect.com/science/article/pii/S2352551724000775 |
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