Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure

Protocol for estimating the contrast transfer function and absolute tilt angle offset for cryo-electron tomography using CTFMeasure

Summary: Contrast transfer function (CTF) estimation is essential to the data processing workflow of cryo-electron tomography (cryoET). Here, we present a protocol for CTF estimation of the cryoET tilt series with CTFMeasure. CTFMeasure can estimate the CTF parameters together with the absolute tilt...

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Bibliographic Details
Main Authors: Ranhao Zhang, Yuan Shen, Xueming Li
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Microscopy
Cryo-EM
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007159
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