Bioreactor Scale-Up and Kinetic Modeling of Lactic Acid and Biomass Production by Enterococcus faecalis SLT13 during Batch Culture on Hydrolyzed Cheese Whey
Kinetic modeling of biomass and lactic acid production by Enterococcus faecalis SLT13 have been developed during batch culture in M17 and Hydrolyzed Cheese Whey (HCW) in 2 L and 20 L bioreactors. The specific growth rate μmax was higher in 20 L bioreactor (1.09 h−1); however, the maximum specific la...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/1236784 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550438669385728 |
|---|---|
| author | Manel Ziadi Sana M’Hir Abdelkarim Aydi Moktar Hamdi |
| author_facet | Manel Ziadi Sana M’Hir Abdelkarim Aydi Moktar Hamdi |
| author_sort | Manel Ziadi |
| collection | DOAJ |
| description | Kinetic modeling of biomass and lactic acid production by Enterococcus faecalis SLT13 have been developed during batch culture in M17 and Hydrolyzed Cheese Whey (HCW) in 2 L and 20 L bioreactors. The specific growth rate μmax was higher in 20 L bioreactor (1.09 h−1); however, the maximum specific lactic acid production rate qpmax and maximum specific sugar utilization rate qsmax were higher in 2 L bioreactor. Biomass and sugar utilization were affected by lactic acid inhibition in HCW. No effects of substrate inhibition have been observed. Substrate limitation of biomass has been observed on HCW in 20 L bioreactor; the substrate limitation constant for biomass Ksx was 4.229 g/L. Substrate limitation of sugar consumption has been observed on M17 in 2 L bioreactor; the substrate limitation constant for sugar consumption Kss was 2.73 g/L. Compared to experimental data, the model provided good predictions for biomass, sugar consumption, and lactic acid production. |
| format | Article |
| id | doaj-art-a235e8d5cf884be5b080fcb7ea343d1a |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-a235e8d5cf884be5b080fcb7ea343d1a2025-02-03T06:06:43ZengWileyJournal of Chemistry2090-90632090-90712020-01-01202010.1155/2020/12367841236784Bioreactor Scale-Up and Kinetic Modeling of Lactic Acid and Biomass Production by Enterococcus faecalis SLT13 during Batch Culture on Hydrolyzed Cheese WheyManel Ziadi0Sana M’Hir1Abdelkarim Aydi2Moktar Hamdi3Laboratory of Microbial Ecology and Technology (LETMi), The National Institute of Applied Sciences and Technology (INSAT), Carthage University, 2 Boulevard de la Terre, BP 676, 1080, Tunis, TunisiaLaboratory of Microbial Ecology and Technology (LETMi), The National Institute of Applied Sciences and Technology (INSAT), Carthage University, 2 Boulevard de la Terre, BP 676, 1080, Tunis, TunisiaLaboratory of Materials Molecules and Applications, Preparatory Institute for Scientific and Technical Studies, Carthage University, BP 51, La Marsa, 2075, Tunis, TunisiaLaboratory of Microbial Ecology and Technology (LETMi), The National Institute of Applied Sciences and Technology (INSAT), Carthage University, 2 Boulevard de la Terre, BP 676, 1080, Tunis, TunisiaKinetic modeling of biomass and lactic acid production by Enterococcus faecalis SLT13 have been developed during batch culture in M17 and Hydrolyzed Cheese Whey (HCW) in 2 L and 20 L bioreactors. The specific growth rate μmax was higher in 20 L bioreactor (1.09 h−1); however, the maximum specific lactic acid production rate qpmax and maximum specific sugar utilization rate qsmax were higher in 2 L bioreactor. Biomass and sugar utilization were affected by lactic acid inhibition in HCW. No effects of substrate inhibition have been observed. Substrate limitation of biomass has been observed on HCW in 20 L bioreactor; the substrate limitation constant for biomass Ksx was 4.229 g/L. Substrate limitation of sugar consumption has been observed on M17 in 2 L bioreactor; the substrate limitation constant for sugar consumption Kss was 2.73 g/L. Compared to experimental data, the model provided good predictions for biomass, sugar consumption, and lactic acid production.http://dx.doi.org/10.1155/2020/1236784 |
| spellingShingle | Manel Ziadi Sana M’Hir Abdelkarim Aydi Moktar Hamdi Bioreactor Scale-Up and Kinetic Modeling of Lactic Acid and Biomass Production by Enterococcus faecalis SLT13 during Batch Culture on Hydrolyzed Cheese Whey Journal of Chemistry |
| title | Bioreactor Scale-Up and Kinetic Modeling of Lactic Acid and Biomass Production by Enterococcus faecalis SLT13 during Batch Culture on Hydrolyzed Cheese Whey |
| title_full | Bioreactor Scale-Up and Kinetic Modeling of Lactic Acid and Biomass Production by Enterococcus faecalis SLT13 during Batch Culture on Hydrolyzed Cheese Whey |
| title_fullStr | Bioreactor Scale-Up and Kinetic Modeling of Lactic Acid and Biomass Production by Enterococcus faecalis SLT13 during Batch Culture on Hydrolyzed Cheese Whey |
| title_full_unstemmed | Bioreactor Scale-Up and Kinetic Modeling of Lactic Acid and Biomass Production by Enterococcus faecalis SLT13 during Batch Culture on Hydrolyzed Cheese Whey |
| title_short | Bioreactor Scale-Up and Kinetic Modeling of Lactic Acid and Biomass Production by Enterococcus faecalis SLT13 during Batch Culture on Hydrolyzed Cheese Whey |
| title_sort | bioreactor scale up and kinetic modeling of lactic acid and biomass production by enterococcus faecalis slt13 during batch culture on hydrolyzed cheese whey |
| url | http://dx.doi.org/10.1155/2020/1236784 |
| work_keys_str_mv | AT manelziadi bioreactorscaleupandkineticmodelingoflacticacidandbiomassproductionbyenterococcusfaecalisslt13duringbatchcultureonhydrolyzedcheesewhey AT sanamhir bioreactorscaleupandkineticmodelingoflacticacidandbiomassproductionbyenterococcusfaecalisslt13duringbatchcultureonhydrolyzedcheesewhey AT abdelkarimaydi bioreactorscaleupandkineticmodelingoflacticacidandbiomassproductionbyenterococcusfaecalisslt13duringbatchcultureonhydrolyzedcheesewhey AT moktarhamdi bioreactorscaleupandkineticmodelingoflacticacidandbiomassproductionbyenterococcusfaecalisslt13duringbatchcultureonhydrolyzedcheesewhey |