In Situ Evaluation of Fludarabine-DNA Interaction Using a DNA-Electrochemical Biosensor
Fludarabine, 9-β-D-arabinosyl-2-fluoroadenine, the nucleoside analog, represents a highly effective treatment for hairy cell leukemia. The electrochemical behaviour of fludarabine is an irreversible diffusion controlled oxidation mechanism and was investigated at a glassy carbon electrode in differe...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Electrochemistry |
| Online Access: | http://dx.doi.org/10.4061/2011/340239 |
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| author | H. Eda Satana Ana Maria Oliveira-Brett |
| author_facet | H. Eda Satana Ana Maria Oliveira-Brett |
| author_sort | H. Eda Satana |
| collection | DOAJ |
| description | Fludarabine, 9-β-D-arabinosyl-2-fluoroadenine, the nucleoside analog, represents a highly effective treatment for hairy cell leukemia. The electrochemical behaviour of fludarabine is an irreversible diffusion controlled oxidation mechanism and was investigated at a glassy carbon electrode in different supporting electrolytes using cyclic, differential pulse, and square wave voltammetry. The diffusion coefficient of fludarabine was calculated to be DFLU=1.71×10−6 cm2 s-1 in pH 7.0 0.1 M phosphate buffer. The oxidation mechanism of fludarabine occurs with the transfer of one proton and one electron and the formation of a hydroxylated species. The interaction of fludarabine with DNA was investigated, by differential pulse voltammetry, in incubated solutions and using dsDNA- and polyhomonucleotides-, poly[G] and poly[A], electrochemical biosensors. The results showed that fludarabine interacts with DNA causing changes in the DNA structure. |
| format | Article |
| id | doaj-art-a22763ee1cfd49d0b866b84b0f928301 |
| institution | Kabale University |
| issn | 2090-3537 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Electrochemistry |
| spelling | doaj-art-a22763ee1cfd49d0b866b84b0f9283012025-02-03T01:27:51ZengWileyInternational Journal of Electrochemistry2090-35372011-01-01201110.4061/2011/340239340239In Situ Evaluation of Fludarabine-DNA Interaction Using a DNA-Electrochemical BiosensorH. Eda Satana0Ana Maria Oliveira-Brett1Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, 3004-535 Coimbra, PortugalDepartamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, 3004-535 Coimbra, PortugalFludarabine, 9-β-D-arabinosyl-2-fluoroadenine, the nucleoside analog, represents a highly effective treatment for hairy cell leukemia. The electrochemical behaviour of fludarabine is an irreversible diffusion controlled oxidation mechanism and was investigated at a glassy carbon electrode in different supporting electrolytes using cyclic, differential pulse, and square wave voltammetry. The diffusion coefficient of fludarabine was calculated to be DFLU=1.71×10−6 cm2 s-1 in pH 7.0 0.1 M phosphate buffer. The oxidation mechanism of fludarabine occurs with the transfer of one proton and one electron and the formation of a hydroxylated species. The interaction of fludarabine with DNA was investigated, by differential pulse voltammetry, in incubated solutions and using dsDNA- and polyhomonucleotides-, poly[G] and poly[A], electrochemical biosensors. The results showed that fludarabine interacts with DNA causing changes in the DNA structure.http://dx.doi.org/10.4061/2011/340239 |
| spellingShingle | H. Eda Satana Ana Maria Oliveira-Brett In Situ Evaluation of Fludarabine-DNA Interaction Using a DNA-Electrochemical Biosensor International Journal of Electrochemistry |
| title | In Situ Evaluation of Fludarabine-DNA Interaction Using a DNA-Electrochemical Biosensor |
| title_full | In Situ Evaluation of Fludarabine-DNA Interaction Using a DNA-Electrochemical Biosensor |
| title_fullStr | In Situ Evaluation of Fludarabine-DNA Interaction Using a DNA-Electrochemical Biosensor |
| title_full_unstemmed | In Situ Evaluation of Fludarabine-DNA Interaction Using a DNA-Electrochemical Biosensor |
| title_short | In Situ Evaluation of Fludarabine-DNA Interaction Using a DNA-Electrochemical Biosensor |
| title_sort | in situ evaluation of fludarabine dna interaction using a dna electrochemical biosensor |
| url | http://dx.doi.org/10.4061/2011/340239 |
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