Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV)
The stress level of vannamei shrimp (Litopenaeus vannamei) is affected by increased density and several genes are expressed under the condition. This study aimed to determine the expression of genes encoding white shrimp stress after density treatment and the infectious myonecrosis virus (IMNV) chal...
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Universitas Airlangga, Faculty of Veterinary Medicine
2024-10-01
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| Series: | Jurnal Medik Veteriner |
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| Online Access: | https://e-journal.unair.ac.id/JMV/article/view/54027 |
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| author | Moch. Nurhudah Nur Komariah Baladrat Sinung Rahardjo Heny Budi Utari Putu Angga Wiradana |
| author_facet | Moch. Nurhudah Nur Komariah Baladrat Sinung Rahardjo Heny Budi Utari Putu Angga Wiradana |
| author_sort | Moch. Nurhudah |
| collection | DOAJ |
| description | The stress level of vannamei shrimp (Litopenaeus vannamei) is affected by increased density and several genes are expressed under the condition. This study aimed to determine the expression of genes encoding white shrimp stress after density treatment and the infectious myonecrosis virus (IMNV) challenge test. A completely randomized design (CRD) was carried out with 6 treatment groups, i.e. 3 different stocking density groups without IMNV infection (100, 200, and 400 shrimp/m2) and 3 different stocking density groups + IMNV infection (100, 200, and 400 shrimp/m2). In addition, a shrimp density of 400 shrimp/m2 reported the fastest rate of developing the IMNV virus as seen from the clinical symptoms. The lowest cumulative number of shrimp deaths was at a density of 100 shrimp/m2 and was caused by the IMNV virus confirmed through RT-PCR. Expression of stress-coding genes was divided into upregulated and downregulated characteristics. The upregulated genes were lectin and translationally controlled tumor protein (TCTP), while the downregulated gene was Toll Receptor. The results showed that the expression of genes related to immunity in L. vannamei was upregulated after pathogen challenges such as lectin and TCTP, meanwhile, the Toll receptor gene was downregulated. Further study should also be performed to measure the expression of the three genes in revealing the immune pathways. |
| format | Article |
| id | doaj-art-a219c6ca96284694b8023efdfd4ecc6a |
| institution | Kabale University |
| issn | 2615-7497 2581-012X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Universitas Airlangga, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Jurnal Medik Veteriner |
| spelling | doaj-art-a219c6ca96284694b8023efdfd4ecc6a2025-02-03T11:58:36ZengUniversitas Airlangga, Faculty of Veterinary MedicineJurnal Medik Veteriner2615-74972581-012X2024-10-017227629110.20473/jmv.vol7.iss2.2024.276-29152189Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV)Moch. Nurhudah0https://orcid.org/0000-0003-3895-4996Nur Komariah Baladrat1https://orcid.org/0000-0001-8723-1546Sinung Rahardjo2https://orcid.org/0000-0002-4698-2623Heny Budi Utari3https://orcid.org/0000-0002-2902-8694Putu Angga Wiradana4https://orcid.org/0000-0002-0139-8781Polytechnic of Marine and Fisheries Karawang, West Java, IndonesiaPostgraduate Program Study of Industrial Aquaculture, Faculty of Fisheries Resources Utilization, Jakarta Technical University of Fisheries, South Jakarta, DKI Jakarta, IndonesiaPolytechnic of Marine and Fisheries Karawang, West Java, IndonesiaAnimal Health Service, Central Proteina Prima Company of Jakarta, DKI Jakarta, IndonesiaResearch Group of Biological Health, Study Program of Biology, Faculty of Health and Science, Universitas Dhyana Pura, Badung, Bali, IndonesiaThe stress level of vannamei shrimp (Litopenaeus vannamei) is affected by increased density and several genes are expressed under the condition. This study aimed to determine the expression of genes encoding white shrimp stress after density treatment and the infectious myonecrosis virus (IMNV) challenge test. A completely randomized design (CRD) was carried out with 6 treatment groups, i.e. 3 different stocking density groups without IMNV infection (100, 200, and 400 shrimp/m2) and 3 different stocking density groups + IMNV infection (100, 200, and 400 shrimp/m2). In addition, a shrimp density of 400 shrimp/m2 reported the fastest rate of developing the IMNV virus as seen from the clinical symptoms. The lowest cumulative number of shrimp deaths was at a density of 100 shrimp/m2 and was caused by the IMNV virus confirmed through RT-PCR. Expression of stress-coding genes was divided into upregulated and downregulated characteristics. The upregulated genes were lectin and translationally controlled tumor protein (TCTP), while the downregulated gene was Toll Receptor. The results showed that the expression of genes related to immunity in L. vannamei was upregulated after pathogen challenges such as lectin and TCTP, meanwhile, the Toll receptor gene was downregulated. Further study should also be performed to measure the expression of the three genes in revealing the immune pathways.https://e-journal.unair.ac.id/JMV/article/view/54027aquaculturegene expressioninfectious myonecrosis viruslitopenaeus vannameistocking density |
| spellingShingle | Moch. Nurhudah Nur Komariah Baladrat Sinung Rahardjo Heny Budi Utari Putu Angga Wiradana Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV) Jurnal Medik Veteriner aquaculture gene expression infectious myonecrosis virus litopenaeus vannamei stocking density |
| title | Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV) |
| title_full | Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV) |
| title_fullStr | Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV) |
| title_full_unstemmed | Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV) |
| title_short | Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV) |
| title_sort | effect of stocking density on stress related gene expression of pacific white shrimp litopenaeus vannamei infected with infectious myonecrosis virus imnv |
| topic | aquaculture gene expression infectious myonecrosis virus litopenaeus vannamei stocking density |
| url | https://e-journal.unair.ac.id/JMV/article/view/54027 |
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