PARAMETERS OF ANTIVIRAL IMMUNITY IN NEWBORNS WITH CYTOMEGALOVIRUS INFECTION IN CASES OF LATE DETECTION OF CYTOMEGALOVIRUS DNA

Objective: study of immune status indices in newborns  in cases of late detection of cytomegalovirus (CMV) DNA in blood and urine.Methods: 147 newborns  with non-specific clinical symptoms were studied. Тyping of lymphocytes relating to different clusters CD3+, CD69+, CD71+, CD95+ using monoclonal a...

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Main Author: L. V. Kravchenko
Format: Article
Language:Russian
Published: LLC "Diagnostics and Vaccines" 2018-09-01
Series:Детские инфекции (Москва)
Subjects:
Online Access:https://detinf.elpub.ru/jour/article/view/374
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author L. V. Kravchenko
author_facet L. V. Kravchenko
author_sort L. V. Kravchenko
collection DOAJ
description Objective: study of immune status indices in newborns  in cases of late detection of cytomegalovirus (CMV) DNA in blood and urine.Methods: 147 newborns  with non-specific clinical symptoms were studied. Тyping of lymphocytes relating to different clusters CD3+, CD69+, CD71+, CD95+ using monoclonal antibodies produced by IMMUNOTECH (France) have been performed. Expression of membrane markers of immunocompetent  cells was measured on a laser flow cytofluorometer Beckman COULTER Epics XL II.Results: In 123 newborns CMVI has been confirmed by the positive result of PCR, while in 24 newborns the negative result was obtained. In 24 three-month old babies, who had a negative  result following DNA diagnostics in the first month  of their life, cytomegalovirus in blood and urine and increase of anti-CMV IgG have been found, which allowed to diagnose CMVI.The following formula reflecting the dependence of CMVI on CD69+ and CD95+ content in newborns having non-specific clinical symptoms in cases of late detection of CMV DNA has been offered.
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spelling doaj-art-a217a1dbc8484bbdb4d05f93b2b70dc32025-08-20T02:46:33ZrusLLC "Diagnostics and Vaccines"Детские инфекции (Москва)2072-81072018-09-01173343710.22627/2072-8107-2018-17-3-34-37350PARAMETERS OF ANTIVIRAL IMMUNITY IN NEWBORNS WITH CYTOMEGALOVIRUS INFECTION IN CASES OF LATE DETECTION OF CYTOMEGALOVIRUS DNAL. V. Kravchenko0Rostov State Medical UniversityObjective: study of immune status indices in newborns  in cases of late detection of cytomegalovirus (CMV) DNA in blood and urine.Methods: 147 newborns  with non-specific clinical symptoms were studied. Тyping of lymphocytes relating to different clusters CD3+, CD69+, CD71+, CD95+ using monoclonal antibodies produced by IMMUNOTECH (France) have been performed. Expression of membrane markers of immunocompetent  cells was measured on a laser flow cytofluorometer Beckman COULTER Epics XL II.Results: In 123 newborns CMVI has been confirmed by the positive result of PCR, while in 24 newborns the negative result was obtained. In 24 three-month old babies, who had a negative  result following DNA diagnostics in the first month  of their life, cytomegalovirus in blood and urine and increase of anti-CMV IgG have been found, which allowed to diagnose CMVI.The following formula reflecting the dependence of CMVI on CD69+ and CD95+ content in newborns having non-specific clinical symptoms in cases of late detection of CMV DNA has been offered.https://detinf.elpub.ru/jour/article/view/374newbornscytomegalovirus infection (cmvi)interferons
spellingShingle L. V. Kravchenko
PARAMETERS OF ANTIVIRAL IMMUNITY IN NEWBORNS WITH CYTOMEGALOVIRUS INFECTION IN CASES OF LATE DETECTION OF CYTOMEGALOVIRUS DNA
Детские инфекции (Москва)
newborns
cytomegalovirus infection (cmvi)
interferons
title PARAMETERS OF ANTIVIRAL IMMUNITY IN NEWBORNS WITH CYTOMEGALOVIRUS INFECTION IN CASES OF LATE DETECTION OF CYTOMEGALOVIRUS DNA
title_full PARAMETERS OF ANTIVIRAL IMMUNITY IN NEWBORNS WITH CYTOMEGALOVIRUS INFECTION IN CASES OF LATE DETECTION OF CYTOMEGALOVIRUS DNA
title_fullStr PARAMETERS OF ANTIVIRAL IMMUNITY IN NEWBORNS WITH CYTOMEGALOVIRUS INFECTION IN CASES OF LATE DETECTION OF CYTOMEGALOVIRUS DNA
title_full_unstemmed PARAMETERS OF ANTIVIRAL IMMUNITY IN NEWBORNS WITH CYTOMEGALOVIRUS INFECTION IN CASES OF LATE DETECTION OF CYTOMEGALOVIRUS DNA
title_short PARAMETERS OF ANTIVIRAL IMMUNITY IN NEWBORNS WITH CYTOMEGALOVIRUS INFECTION IN CASES OF LATE DETECTION OF CYTOMEGALOVIRUS DNA
title_sort parameters of antiviral immunity in newborns with cytomegalovirus infection in cases of late detection of cytomegalovirus dna
topic newborns
cytomegalovirus infection (cmvi)
interferons
url https://detinf.elpub.ru/jour/article/view/374
work_keys_str_mv AT lvkravchenko parametersofantiviralimmunityinnewbornswithcytomegalovirusinfectionincasesoflatedetectionofcytomegalovirusdna