Prospects for Using Atomic Power Microscopic Methods in Reanimatology
Objective: to obtain an image of pores in the membrane of an erythrocyte after its electroporation, by using an atomic power microscope (APM), to evaluate the parameters of the obtained pores, and to discuss the prospects for using an APM in reanimatology. Human whole venous blood was exposed to a p...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2008-08-01
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| Series: | Общая реаниматология |
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| Online Access: | https://www.reanimatology.com/rmt/article/view/711 |
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| author | V. V. Moroz A. M. Chernysh I. V. Yaminsky Ye. K. Kozlova G. A. Kiselev A. S. Filonov M. S. Bogushevich O. Ye. Gudkova |
| author_facet | V. V. Moroz A. M. Chernysh I. V. Yaminsky Ye. K. Kozlova G. A. Kiselev A. S. Filonov M. S. Bogushevich O. Ye. Gudkova |
| author_sort | V. V. Moroz |
| collection | DOAJ |
| description | Objective: to obtain an image of pores in the membrane of an erythrocyte after its electroporation, by using an atomic power microscope (APM), to evaluate the parameters of the obtained pores, and to discuss the prospects for using an APM in reanimatology. Human whole venous blood was exposed to a pulsed electric field, by inducing erythrocytic membrane elec-troporation. Blood smears were obtained by the standard method. Images of the form and surface of individual erythrocytes and their enlarged fragments were obtained by means of a Femto Scan APM. The diameters, depths, and configuration of some pores were analyzed. This example and others were used to show a wide field and prospects for using an APM in reanimatology. |
| format | Article |
| id | doaj-art-a21506430422416e983e3004e20a1f87 |
| institution | DOAJ |
| issn | 1813-9779 2411-7110 |
| language | English |
| publishDate | 2008-08-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj-art-a21506430422416e983e3004e20a1f872025-08-20T02:55:52ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102008-08-014410.15360/1813-9779-2008-4-51711Prospects for Using Atomic Power Microscopic Methods in ReanimatologyV. V. MorozA. M. ChernyshI. V. YaminskyYe. K. KozlovaG. A. KiselevA. S. FilonovM. S. BogushevichO. Ye. GudkovaObjective: to obtain an image of pores in the membrane of an erythrocyte after its electroporation, by using an atomic power microscope (APM), to evaluate the parameters of the obtained pores, and to discuss the prospects for using an APM in reanimatology. Human whole venous blood was exposed to a pulsed electric field, by inducing erythrocytic membrane elec-troporation. Blood smears were obtained by the standard method. Images of the form and surface of individual erythrocytes and their enlarged fragments were obtained by means of a Femto Scan APM. The diameters, depths, and configuration of some pores were analyzed. This example and others were used to show a wide field and prospects for using an APM in reanimatology.https://www.reanimatology.com/rmt/article/view/711atomic power microscopemembrane electroporationanalysis of pores. |
| spellingShingle | V. V. Moroz A. M. Chernysh I. V. Yaminsky Ye. K. Kozlova G. A. Kiselev A. S. Filonov M. S. Bogushevich O. Ye. Gudkova Prospects for Using Atomic Power Microscopic Methods in Reanimatology Общая реаниматология atomic power microscope membrane electroporation analysis of pores. |
| title | Prospects for Using Atomic Power Microscopic Methods in Reanimatology |
| title_full | Prospects for Using Atomic Power Microscopic Methods in Reanimatology |
| title_fullStr | Prospects for Using Atomic Power Microscopic Methods in Reanimatology |
| title_full_unstemmed | Prospects for Using Atomic Power Microscopic Methods in Reanimatology |
| title_short | Prospects for Using Atomic Power Microscopic Methods in Reanimatology |
| title_sort | prospects for using atomic power microscopic methods in reanimatology |
| topic | atomic power microscope membrane electroporation analysis of pores. |
| url | https://www.reanimatology.com/rmt/article/view/711 |
| work_keys_str_mv | AT vvmoroz prospectsforusingatomicpowermicroscopicmethodsinreanimatology AT amchernysh prospectsforusingatomicpowermicroscopicmethodsinreanimatology AT ivyaminsky prospectsforusingatomicpowermicroscopicmethodsinreanimatology AT yekkozlova prospectsforusingatomicpowermicroscopicmethodsinreanimatology AT gakiselev prospectsforusingatomicpowermicroscopicmethodsinreanimatology AT asfilonov prospectsforusingatomicpowermicroscopicmethodsinreanimatology AT msbogushevich prospectsforusingatomicpowermicroscopicmethodsinreanimatology AT oyegudkova prospectsforusingatomicpowermicroscopicmethodsinreanimatology |