Comparative Analysis of Tunicate vs. Plant-Based Cellulose in Chitosan Hydrogels for Bone Regeneration
A novel hydrogel scaffold for bone regeneration based on chitosan, selected for its biocompatibility, biodegradability, and antimicrobial properties, was covalently functionalized with a bioactive peptide from bone morphogenetic protein-2 (BMP-2) to guide osteoblast growth and proliferation. This st...
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MDPI AG
2025-02-01
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| Online Access: | https://www.mdpi.com/2310-2861/11/2/102 |
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| author | Laura Furlan Annj Zamuner Andrea Riccioni Giacomo Sabbadin Teresa Russo Vito Gallicchio Gabriella D’Auria Lucia Falcigno Lucia Manni Loriano Ballarin Elisabetta Schievano Paola Brun Monica Dettin |
| author_facet | Laura Furlan Annj Zamuner Andrea Riccioni Giacomo Sabbadin Teresa Russo Vito Gallicchio Gabriella D’Auria Lucia Falcigno Lucia Manni Loriano Ballarin Elisabetta Schievano Paola Brun Monica Dettin |
| author_sort | Laura Furlan |
| collection | DOAJ |
| description | A novel hydrogel scaffold for bone regeneration based on chitosan, selected for its biocompatibility, biodegradability, and antimicrobial properties, was covalently functionalized with a bioactive peptide from bone morphogenetic protein-2 (BMP-2) to guide osteoblast growth and proliferation. This study evaluates the impact of incorporating different concentrations (8, 16, or 24% <i>wt</i>/<i>wt</i>) of plant-based micro-fibrillated cellulose or tunicate nanocellulose to improve the mechanical and biological properties of peptide-grafted chitosan hydrogel matrices. While the mechanical properties of the matrices increase with increasing cellulose content, regardless of its source, the behavior of human osteoblasts used in biological tests discriminates between the two types of cellulose and shows better results (proliferation at 2 and 7 days, and mineralization) for the enrichment with tunicate cellulose. |
| format | Article |
| id | doaj-art-a2053382c8e446378d4db7f66efe5afc |
| institution | DOAJ |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-a2053382c8e446378d4db7f66efe5afc2025-08-20T02:45:00ZengMDPI AGGels2310-28612025-02-0111210210.3390/gels11020102Comparative Analysis of Tunicate vs. Plant-Based Cellulose in Chitosan Hydrogels for Bone RegenerationLaura Furlan0Annj Zamuner1Andrea Riccioni2Giacomo Sabbadin3Teresa Russo4Vito Gallicchio5Gabriella D’Auria6Lucia Falcigno7Lucia Manni8Loriano Ballarin9Elisabetta Schievano10Paola Brun11Monica Dettin12Department of Industrial Engineering, University of Padova, 35131 Padova, ItalyDepartment of Industrial Engineering, University of Padova, 35131 Padova, ItalyDepartment of Chemical Sciences, University of Padova, 35131 Padova, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyInstitute of Polymers, Composites and Biomaterials, National Research Council of Italy, 80125 Naples, ItalyDepartment of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, 80131 Naples, ItalyDepartment of Pharmacy, University Federico II of Naples, 80131 Naples, ItalyDepartment of Pharmacy, University Federico II of Naples, 80131 Naples, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyDepartment of Biology, University of Padova, 35131 Padova, ItalyDepartment of Chemical Sciences, University of Padova, 35131 Padova, ItalyDepartment of Molecular Medicine, University of Padova, 35121 Padua, ItalyDepartment of Industrial Engineering, University of Padova, 35131 Padova, ItalyA novel hydrogel scaffold for bone regeneration based on chitosan, selected for its biocompatibility, biodegradability, and antimicrobial properties, was covalently functionalized with a bioactive peptide from bone morphogenetic protein-2 (BMP-2) to guide osteoblast growth and proliferation. This study evaluates the impact of incorporating different concentrations (8, 16, or 24% <i>wt</i>/<i>wt</i>) of plant-based micro-fibrillated cellulose or tunicate nanocellulose to improve the mechanical and biological properties of peptide-grafted chitosan hydrogel matrices. While the mechanical properties of the matrices increase with increasing cellulose content, regardless of its source, the behavior of human osteoblasts used in biological tests discriminates between the two types of cellulose and shows better results (proliferation at 2 and 7 days, and mineralization) for the enrichment with tunicate cellulose.https://www.mdpi.com/2310-2861/11/2/102plant-based cellulosetunicate cellulosechitosanhydrogelBMP-2bone tissue engineering |
| spellingShingle | Laura Furlan Annj Zamuner Andrea Riccioni Giacomo Sabbadin Teresa Russo Vito Gallicchio Gabriella D’Auria Lucia Falcigno Lucia Manni Loriano Ballarin Elisabetta Schievano Paola Brun Monica Dettin Comparative Analysis of Tunicate vs. Plant-Based Cellulose in Chitosan Hydrogels for Bone Regeneration Gels plant-based cellulose tunicate cellulose chitosan hydrogel BMP-2 bone tissue engineering |
| title | Comparative Analysis of Tunicate vs. Plant-Based Cellulose in Chitosan Hydrogels for Bone Regeneration |
| title_full | Comparative Analysis of Tunicate vs. Plant-Based Cellulose in Chitosan Hydrogels for Bone Regeneration |
| title_fullStr | Comparative Analysis of Tunicate vs. Plant-Based Cellulose in Chitosan Hydrogels for Bone Regeneration |
| title_full_unstemmed | Comparative Analysis of Tunicate vs. Plant-Based Cellulose in Chitosan Hydrogels for Bone Regeneration |
| title_short | Comparative Analysis of Tunicate vs. Plant-Based Cellulose in Chitosan Hydrogels for Bone Regeneration |
| title_sort | comparative analysis of tunicate vs plant based cellulose in chitosan hydrogels for bone regeneration |
| topic | plant-based cellulose tunicate cellulose chitosan hydrogel BMP-2 bone tissue engineering |
| url | https://www.mdpi.com/2310-2861/11/2/102 |
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