Identification and Characterization of Antiviral Activity of Synthetic Compounds Against Mayaro Virus
<b>Background/objectives:</b> In Brazil, the co-circulation of arboviruses—such as dengue, Zika, yellow fever, and Chikungunya viruses—creates a complex epidemiological landscape, drawing attention from health authorities due to high morbidity and mortality rates. Also present in this co...
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2025-05-01
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| author | Ana Paula Andreolla Andrea Cristine Koishi Alessandra Abel Borges Larissa Albuquerque de Oliveira Viviane Guedes de Oliveira Nerilson Marques Lima Eloah Pereira Ávila Pedro Pôssa de Castro Giovanni Wilson Amarante Mauro Vieira de Almeida Juliano Bordignon Claudia Nunes Duarte dos Santos |
| author_facet | Ana Paula Andreolla Andrea Cristine Koishi Alessandra Abel Borges Larissa Albuquerque de Oliveira Viviane Guedes de Oliveira Nerilson Marques Lima Eloah Pereira Ávila Pedro Pôssa de Castro Giovanni Wilson Amarante Mauro Vieira de Almeida Juliano Bordignon Claudia Nunes Duarte dos Santos |
| author_sort | Ana Paula Andreolla |
| collection | DOAJ |
| description | <b>Background/objectives:</b> In Brazil, the co-circulation of arboviruses—such as dengue, Zika, yellow fever, and Chikungunya viruses—creates a complex epidemiological landscape, drawing attention from health authorities due to high morbidity and mortality rates. Also present in this context is the Mayaro virus (MAYV), a neglected arbovirus, which can also cause severe syndromes and has been expanding beyond its usual endemic areas in northern and central-western Brazil. Epidemiological surveillance measures remain limited, and there are no effective prophylactic strategies or antiviral treatments for this neglected arbovirus. In this study, we evaluated the antiviral activity of commercial and synthetic compounds against MAYV using an image high-throughput screening (iHTS) system. <b>Methods:</b> A total of 52 compounds from an FDA-approved commercial library (Tocriscreen) and 50 other compounds were tested. <b>Results:</b> Seven compounds showed anti-MAYV activity and were non-toxic for the following cell lines: Naringenin, LLA9A, chrysin, and its ester C6. Post-infection treatments with these selected compounds significantly decreased the percentage of infected cells and the release of infectious viral particles in the supernatant. Additionally, anti-MAYV activity of these four selected hits was confirmed using several human cell lines and two different MAYV genotypes. <b>Conclusions:</b> Our results indicate that the iHTS platform is effective for screening anti-MAYV drugs and that four promising compounds can efficiently inhibit MAYV replication in human cell lines. Although in vivo studies are still required to confirm the efficacy of the selected hits, our findings provide a starting point for developing a potential treatment for MAYV infections. |
| format | Article |
| id | doaj-art-a203e93e9bac4e619175194e7739c8d8 |
| institution | DOAJ |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-a203e93e9bac4e619175194e7739c8d82025-08-20T03:14:29ZengMDPI AGPharmaceuticals1424-82472025-05-0118571710.3390/ph18050717Identification and Characterization of Antiviral Activity of Synthetic Compounds Against Mayaro VirusAna Paula Andreolla0Andrea Cristine Koishi1Alessandra Abel Borges2Larissa Albuquerque de Oliveira3Viviane Guedes de Oliveira4Nerilson Marques Lima5Eloah Pereira Ávila6Pedro Pôssa de Castro7Giovanni Wilson Amarante8Mauro Vieira de Almeida9Juliano Bordignon10Claudia Nunes Duarte dos Santos11Laboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Cidade Industrial de Curitiba, Curitiba 81350-010, Paraná, BrazilLaboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Cidade Industrial de Curitiba, Curitiba 81350-010, Paraná, BrazilLaboratório de Pesquisas em Virologia e Imunologia, Instituto de Ciências Biológicas e da Saúde (ICBS), Universidade Federal de Alagoas (UFAL), Av. Lourival Melo Mota, s/n, Tabuleiro do Martins, Maceió 57072-900, Alagoas, BrazilDepartamento de Química, ICE, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer s/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, BrazilInstituto de Educação, Agricultura e Ambiente, IEAA, Universidade Federal do Amazonas, Rua 29 de agosto, Centro, Humaitá 69800–000, Amazonas, BrazilDepartamento de Química, ICE, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer s/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, BrazilDepartamento de Química, ICE, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer s/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, BrazilDepartamento de Química, ICE, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer s/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, BrazilDepartamento de Química, ICE, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer s/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, BrazilDepartamento de Química, ICE, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer s/n, Martelos, Juiz de Fora 36036-900, Minas Gerais, BrazilLaboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Cidade Industrial de Curitiba, Curitiba 81350-010, Paraná, BrazilLaboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz, Rua Prof. Algacyr Munhoz Mader 3775, Cidade Industrial de Curitiba, Curitiba 81350-010, Paraná, Brazil<b>Background/objectives:</b> In Brazil, the co-circulation of arboviruses—such as dengue, Zika, yellow fever, and Chikungunya viruses—creates a complex epidemiological landscape, drawing attention from health authorities due to high morbidity and mortality rates. Also present in this context is the Mayaro virus (MAYV), a neglected arbovirus, which can also cause severe syndromes and has been expanding beyond its usual endemic areas in northern and central-western Brazil. Epidemiological surveillance measures remain limited, and there are no effective prophylactic strategies or antiviral treatments for this neglected arbovirus. In this study, we evaluated the antiviral activity of commercial and synthetic compounds against MAYV using an image high-throughput screening (iHTS) system. <b>Methods:</b> A total of 52 compounds from an FDA-approved commercial library (Tocriscreen) and 50 other compounds were tested. <b>Results:</b> Seven compounds showed anti-MAYV activity and were non-toxic for the following cell lines: Naringenin, LLA9A, chrysin, and its ester C6. Post-infection treatments with these selected compounds significantly decreased the percentage of infected cells and the release of infectious viral particles in the supernatant. Additionally, anti-MAYV activity of these four selected hits was confirmed using several human cell lines and two different MAYV genotypes. <b>Conclusions:</b> Our results indicate that the iHTS platform is effective for screening anti-MAYV drugs and that four promising compounds can efficiently inhibit MAYV replication in human cell lines. Although in vivo studies are still required to confirm the efficacy of the selected hits, our findings provide a starting point for developing a potential treatment for MAYV infections.https://www.mdpi.com/1424-8247/18/5/717arbovirusalphavirusMayaro virusantiviral drugsiHTS assayflavonoids |
| spellingShingle | Ana Paula Andreolla Andrea Cristine Koishi Alessandra Abel Borges Larissa Albuquerque de Oliveira Viviane Guedes de Oliveira Nerilson Marques Lima Eloah Pereira Ávila Pedro Pôssa de Castro Giovanni Wilson Amarante Mauro Vieira de Almeida Juliano Bordignon Claudia Nunes Duarte dos Santos Identification and Characterization of Antiviral Activity of Synthetic Compounds Against Mayaro Virus Pharmaceuticals arbovirus alphavirus Mayaro virus antiviral drugs iHTS assay flavonoids |
| title | Identification and Characterization of Antiviral Activity of Synthetic Compounds Against Mayaro Virus |
| title_full | Identification and Characterization of Antiviral Activity of Synthetic Compounds Against Mayaro Virus |
| title_fullStr | Identification and Characterization of Antiviral Activity of Synthetic Compounds Against Mayaro Virus |
| title_full_unstemmed | Identification and Characterization of Antiviral Activity of Synthetic Compounds Against Mayaro Virus |
| title_short | Identification and Characterization of Antiviral Activity of Synthetic Compounds Against Mayaro Virus |
| title_sort | identification and characterization of antiviral activity of synthetic compounds against mayaro virus |
| topic | arbovirus alphavirus Mayaro virus antiviral drugs iHTS assay flavonoids |
| url | https://www.mdpi.com/1424-8247/18/5/717 |
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