Genotype-Dependent Variations in Oxidative Stress Markers and Bioactive Proteins in Hereford Bulls: Associations with <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> Genes
The objective of this study is to assess the influence of genetic polymorphisms in <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> on the oxidative stress biomarkers and bioactive protein levels in Hereford bulls. A total of sixty-eight bulls were analyzed at 22 months...
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2024-10-01
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| author | Piotr Kostusiak Emilia Bagnicka Beata Żelazowska Magdalena Zalewska Tomasz Sakowski Jan Slósarz Marcin Gołębiewski Kamila Puppel |
| author_facet | Piotr Kostusiak Emilia Bagnicka Beata Żelazowska Magdalena Zalewska Tomasz Sakowski Jan Slósarz Marcin Gołębiewski Kamila Puppel |
| author_sort | Piotr Kostusiak |
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| description | The objective of this study is to assess the influence of genetic polymorphisms in <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> on the oxidative stress biomarkers and bioactive protein levels in Hereford bulls. A total of sixty-eight bulls were analyzed at 22 months of age to assess growth metrics and carcass quality, with a focus on polymorphisms in these genes. The key markers of oxidative stress, including malondialdehyde (MDA), and the activities of antioxidant enzymes such as glutathione reductase (GluRed), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were measured, alongside bioactive compounds like taurine, carnosine, and anserine. The results show that the TT genotype of <i>DGAT1</i> is linked to significantly higher MDA levels, reflecting increased lipid peroxidation, but is also associated with higher GluRed and GPx activities and elevated levels of taurine, carnosine, and anserine, suggesting an adaptive response to oxidative stress. The <i>LEP</i> gene analysis revealed that the CC genotype had the highest MDA levels but also exhibited increased GPx and SOD activities, with the CT genotype showing the highest SOD activity and the TT genotype the highest total antioxidant status (TAS). The <i>SCD1</i> AA genotype displayed the highest activities of GluRed, GPx, and SOD, indicating a more effective antioxidant defence, while the VA genotype had the highest MDA levels and the VV genotype showed lower MDA levels, suggesting protective effects against oxidative damage. These findings highlight genotype specific variations in the oxidative stress markers and bioactive compound levels, providing insights into the genetic regulation of oxidative stress and antioxidant defences, which could inform breeding strategies for improving oxidative stress resistance in livestock and managing related conditions. |
| format | Article |
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| institution | OA Journals |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-a1f6422c03b24615bca456fd6df90e2c2025-08-20T02:11:14ZengMDPI AGBiomolecules2218-273X2024-10-011410130910.3390/biom14101309Genotype-Dependent Variations in Oxidative Stress Markers and Bioactive Proteins in Hereford Bulls: Associations with <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> GenesPiotr Kostusiak0Emilia Bagnicka1Beata Żelazowska2Magdalena Zalewska3Tomasz Sakowski4Jan Slósarz5Marcin Gołębiewski6Kamila Puppel7Institute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandInstitute of Genetics and Animal Biotechnology, Polish Academy of Science, Jastrzębiec, Postępu 36A, 05-552 Magdalenka, PolandInstitute of Genetics and Animal Biotechnology, Polish Academy of Science, Jastrzębiec, Postępu 36A, 05-552 Magdalenka, PolandDepartment of Bacterial Physiology, Institute of Microbiology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, PolandInstitute of Genetics and Animal Biotechnology, Polish Academy of Science, Jastrzębiec, Postępu 36A, 05-552 Magdalenka, PolandInstitute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandInstitute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandInstitute of Animal Science, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, PolandThe objective of this study is to assess the influence of genetic polymorphisms in <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> on the oxidative stress biomarkers and bioactive protein levels in Hereford bulls. A total of sixty-eight bulls were analyzed at 22 months of age to assess growth metrics and carcass quality, with a focus on polymorphisms in these genes. The key markers of oxidative stress, including malondialdehyde (MDA), and the activities of antioxidant enzymes such as glutathione reductase (GluRed), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were measured, alongside bioactive compounds like taurine, carnosine, and anserine. The results show that the TT genotype of <i>DGAT1</i> is linked to significantly higher MDA levels, reflecting increased lipid peroxidation, but is also associated with higher GluRed and GPx activities and elevated levels of taurine, carnosine, and anserine, suggesting an adaptive response to oxidative stress. The <i>LEP</i> gene analysis revealed that the CC genotype had the highest MDA levels but also exhibited increased GPx and SOD activities, with the CT genotype showing the highest SOD activity and the TT genotype the highest total antioxidant status (TAS). The <i>SCD1</i> AA genotype displayed the highest activities of GluRed, GPx, and SOD, indicating a more effective antioxidant defence, while the VA genotype had the highest MDA levels and the VV genotype showed lower MDA levels, suggesting protective effects against oxidative damage. These findings highlight genotype specific variations in the oxidative stress markers and bioactive compound levels, providing insights into the genetic regulation of oxidative stress and antioxidant defences, which could inform breeding strategies for improving oxidative stress resistance in livestock and managing related conditions.https://www.mdpi.com/2218-273X/14/10/1309genetic variations<i>DGAT1</i><i>LEP</i><i>SCD1</i>oxidative stressbeef |
| spellingShingle | Piotr Kostusiak Emilia Bagnicka Beata Żelazowska Magdalena Zalewska Tomasz Sakowski Jan Slósarz Marcin Gołębiewski Kamila Puppel Genotype-Dependent Variations in Oxidative Stress Markers and Bioactive Proteins in Hereford Bulls: Associations with <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> Genes Biomolecules genetic variations <i>DGAT1</i> <i>LEP</i> <i>SCD1</i> oxidative stress beef |
| title | Genotype-Dependent Variations in Oxidative Stress Markers and Bioactive Proteins in Hereford Bulls: Associations with <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> Genes |
| title_full | Genotype-Dependent Variations in Oxidative Stress Markers and Bioactive Proteins in Hereford Bulls: Associations with <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> Genes |
| title_fullStr | Genotype-Dependent Variations in Oxidative Stress Markers and Bioactive Proteins in Hereford Bulls: Associations with <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> Genes |
| title_full_unstemmed | Genotype-Dependent Variations in Oxidative Stress Markers and Bioactive Proteins in Hereford Bulls: Associations with <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> Genes |
| title_short | Genotype-Dependent Variations in Oxidative Stress Markers and Bioactive Proteins in Hereford Bulls: Associations with <i>DGAT1</i>, <i>LEP</i>, and <i>SCD1</i> Genes |
| title_sort | genotype dependent variations in oxidative stress markers and bioactive proteins in hereford bulls associations with i dgat1 i i lep i and i scd1 i genes |
| topic | genetic variations <i>DGAT1</i> <i>LEP</i> <i>SCD1</i> oxidative stress beef |
| url | https://www.mdpi.com/2218-273X/14/10/1309 |
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