Study on the Immunogenicity of Infectious Bursal Disease Virus VP2 proteins Expressed in Silkworm
The VP2 protein of infectious bursal disease virus (IBDV) was expressed in spring and autumn silkworm larvae as detected by Western blot and ELISA. Non-immunized 18-day-old chickens were vaccinated via subcutaneous injection with a vaccine preparation made from the lymph of spring silkworms containi...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2000-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2000.01.0009 |
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| author | YU Lian SONG Kun-hua ZHANG Yao-zhou HUANG Yao-wei LI Jian-rong |
| author_facet | YU Lian SONG Kun-hua ZHANG Yao-zhou HUANG Yao-wei LI Jian-rong |
| author_sort | YU Lian |
| collection | DOAJ |
| description | The VP2 protein of infectious bursal disease virus (IBDV) was expressed in spring and autumn silkworm larvae as detected by Western blot and ELISA. Non-immunized 18-day-old chickens were vaccinated via subcutaneous injection with a vaccine preparation made from the lymph of spring silkworms containing VP2 expression product. The boosting immunization was carried out two weeks later. The lymph of autumn silkworms was freeze-dried as the oral vaccine. Non-immunized 18-day-old chickens were fed on the oral vaccine every other day. Serum samples from the immunized chickens were harvested. Virus neutralization (VN) test showed that the titers of VN antibody was 1∶4211.4 in the sera of chickens receiving subcutaneous immunization and 1∶3118.9 in those of chickens fed on oral vaccine at day 37 post-immunization. The immunized chickens were challenged with BC6/85, a reference Chinese virulent IBDV strain at the 37th day after vaccination. The rate of protection against BC6/85 infection was 100% for the chickens with subcutaneous vaccination and 80% for those receiving oral vaccine. These results indicate that the VP2 protein expressed in silkworm larvae is immunogenic and the established expression system for VP2 has laid a firm basis for the development of practical yet low-cost vaccines against infectious bursal disease. |
| format | Article |
| id | doaj-art-a1d3d793df3a45efb0af7601a3bd02c7 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-a1d3d793df3a45efb0af7601a3bd02c72025-08-20T02:54:53ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552000-01-012691610.3785/1008-9209.2000.01.000910089209Study on the Immunogenicity of Infectious Bursal Disease Virus VP2 proteins Expressed in SilkwormYU LianSONG Kun-huaZHANG Yao-zhouHUANG Yao-weiLI Jian-rongThe VP2 protein of infectious bursal disease virus (IBDV) was expressed in spring and autumn silkworm larvae as detected by Western blot and ELISA. Non-immunized 18-day-old chickens were vaccinated via subcutaneous injection with a vaccine preparation made from the lymph of spring silkworms containing VP2 expression product. The boosting immunization was carried out two weeks later. The lymph of autumn silkworms was freeze-dried as the oral vaccine. Non-immunized 18-day-old chickens were fed on the oral vaccine every other day. Serum samples from the immunized chickens were harvested. Virus neutralization (VN) test showed that the titers of VN antibody was 1∶4211.4 in the sera of chickens receiving subcutaneous immunization and 1∶3118.9 in those of chickens fed on oral vaccine at day 37 post-immunization. The immunized chickens were challenged with BC6/85, a reference Chinese virulent IBDV strain at the 37th day after vaccination. The rate of protection against BC6/85 infection was 100% for the chickens with subcutaneous vaccination and 80% for those receiving oral vaccine. These results indicate that the VP2 protein expressed in silkworm larvae is immunogenic and the established expression system for VP2 has laid a firm basis for the development of practical yet low-cost vaccines against infectious bursal disease.https://www.academax.com/doi/10.3785/1008-9209.2000.01.0009infectious bursal disease virus (IBDV)VP2 geneimmunogenicitysilkwormrecombinant subunit vaccine |
| spellingShingle | YU Lian SONG Kun-hua ZHANG Yao-zhou HUANG Yao-wei LI Jian-rong Study on the Immunogenicity of Infectious Bursal Disease Virus VP2 proteins Expressed in Silkworm 浙江大学学报. 农业与生命科学版 infectious bursal disease virus (IBDV) VP2 gene immunogenicity silkworm recombinant subunit vaccine |
| title | Study on the Immunogenicity of Infectious Bursal Disease Virus VP2 proteins Expressed in Silkworm |
| title_full | Study on the Immunogenicity of Infectious Bursal Disease Virus VP2 proteins Expressed in Silkworm |
| title_fullStr | Study on the Immunogenicity of Infectious Bursal Disease Virus VP2 proteins Expressed in Silkworm |
| title_full_unstemmed | Study on the Immunogenicity of Infectious Bursal Disease Virus VP2 proteins Expressed in Silkworm |
| title_short | Study on the Immunogenicity of Infectious Bursal Disease Virus VP2 proteins Expressed in Silkworm |
| title_sort | study on the immunogenicity of infectious bursal disease virus vp2 proteins expressed in silkworm |
| topic | infectious bursal disease virus (IBDV) VP2 gene immunogenicity silkworm recombinant subunit vaccine |
| url | https://www.academax.com/doi/10.3785/1008-9209.2000.01.0009 |
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