Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust Preparation
The development of the underpinning methodology for the production of robust, well-formed, and densely packed biotin-HPDP functionalised gold surfaces, the crucial first step in immobilising bimolecules on surfaces, is described. Self-assembled monolayers (SAMs) with biotin end-groups were prepared...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2006-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2006.20 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849398521330925568 |
|---|---|
| author | Richard J.C. Brown Dan J.L. Brett |
| author_facet | Richard J.C. Brown Dan J.L. Brett |
| author_sort | Richard J.C. Brown |
| collection | DOAJ |
| description | The development of the underpinning methodology for the production of robust, well-formed, and densely packed biotin-HPDP functionalised gold surfaces, the crucial first step in immobilising bimolecules on surfaces, is described. Self-assembled monolayers (SAMs) with biotin end-groups were prepared on polycrystalline gold surfaces according to a published method. The layers formed were studied using cyclic voltammetry to determine the composition of the layer and its quality. Crystal impedance spectroscopy was also applied as a complimentary indicator of the composition of the layer.For the first time, the effect of assembly time on the properties of the layer was studied along with the composition of the layer and the ability of the precursor molecule to self-assemble by oxidative addition. |
| format | Article |
| id | doaj-art-a1d3002fb8a34686bbb245923132fc62 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2006-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a1d3002fb8a34686bbb245923132fc622025-08-20T03:38:35ZengWileyThe Scientific World Journal1537-744X2006-01-016202910.1100/tsw.2006.20Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust PreparationRichard J.C. Brown0Dan J.L. Brett1Analytical Science Group, National Physical Laboratory, Teddington, Middlesex, TW11 0LW, UKDepartment of Chemical Engineering and Chemical Technology, Imperial College, London, SW7 2AY, USAThe development of the underpinning methodology for the production of robust, well-formed, and densely packed biotin-HPDP functionalised gold surfaces, the crucial first step in immobilising bimolecules on surfaces, is described. Self-assembled monolayers (SAMs) with biotin end-groups were prepared on polycrystalline gold surfaces according to a published method. The layers formed were studied using cyclic voltammetry to determine the composition of the layer and its quality. Crystal impedance spectroscopy was also applied as a complimentary indicator of the composition of the layer.For the first time, the effect of assembly time on the properties of the layer was studied along with the composition of the layer and the ability of the precursor molecule to self-assemble by oxidative addition.http://dx.doi.org/10.1100/tsw.2006.20 |
| spellingShingle | Richard J.C. Brown Dan J.L. Brett Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust Preparation The Scientific World Journal |
| title | Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust Preparation |
| title_full | Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust Preparation |
| title_fullStr | Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust Preparation |
| title_full_unstemmed | Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust Preparation |
| title_short | Electrochemical Study of Biotin-Modified Self-Assembled Monolayers: Recommendations for Robust Preparation |
| title_sort | electrochemical study of biotin modified self assembled monolayers recommendations for robust preparation |
| url | http://dx.doi.org/10.1100/tsw.2006.20 |
| work_keys_str_mv | AT richardjcbrown electrochemicalstudyofbiotinmodifiedselfassembledmonolayersrecommendationsforrobustpreparation AT danjlbrett electrochemicalstudyofbiotinmodifiedselfassembledmonolayersrecommendationsforrobustpreparation |