Production of Shiga-Toxin by Escherichia coli O157:H7 isolated from Raw Cow Milk obtained within Kaduna Metropolis, Nigeria
Study’s Excerpt: • coli O157:H7 detected in raw cow milk poses a public health risk. • Isolates harbored both stx1 and stx2 virulence genes. • Biofilm formation enhances the pathogenicity of STEC strains. • Essential oils impaired biofilm, with cinnamon showing strongest effect. • Phyt...
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| Format: | Article |
| Language: | English |
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Umaru Musa Yar'adua University, Katsina, Nigeria
2025-06-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1169 |
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| author | Abubakar, S. S. Maiangwa, J. Idris, S. Musa, J. |
| author_facet | Abubakar, S. S. Maiangwa, J. Idris, S. Musa, J. |
| author_sort | Abubakar, S. S. |
| collection | DOAJ |
| description |
Study’s Excerpt:
• coli O157:H7 detected in raw cow milk poses a public health risk.
• Isolates harbored both stx1 and stx2 virulence genes.
• Biofilm formation enhances the pathogenicity of STEC strains.
• Essential oils impaired biofilm, with cinnamon showing strongest effect.
• Phytochemicals may help reduce STEC virulence in dairy products.
Full Abstract:
Shiga toxin-producing Escherichia coli O157: H7 (STEC) is a food-borne pathogen that is associated with human illnesses from mild gastroenteritis to severe life-threatening hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. Its pathogenesis can be enhanced by the occurrence of certain virulence genes and the ability to produce biofilm. The present study investigates the spectrum of such pathogenesis on the basis of the occurrence of shiga-toxigenic E. coli O157:H7 in raw cow milk obtained from cattle herds. Furthermore, the inhibitory potential of some selected essential oils against their biofilm formation was determined. Results revealed isolated shiga-toxigenic E. coli O157:H7 harboured the stx1 and stx2 virulence genes. Inhibitory assessments of cinnamon, clove, eucalyptus, and tea tree oils also revealed impairment of biofilm formation at 1%, 2% and 3% concentrations, with cinnamon having a higher inhibitory impact. The presence of E. coli 0157:H7 in raw cow milk suggests that they are not restricted to contaminating raw meat only, but can be a contaminant of dairy products as well. The combined effect of stx1, stx2, and biofilm formation is of public health concern as plasmid transfer among strains could result in antimicrobial resistance. Phytochemicals from plants such as cinnamon, clove, eucalyptus, and tea tree could play vital biofilm inhibitory roles in decreasing the virulence of E. coli 0157:H7.
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| format | Article |
| id | doaj-art-a1cfbe4c9f17458c83cda183d172bda8 |
| institution | Kabale University |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-a1cfbe4c9f17458c83cda183d172bda82025-08-22T14:14:36ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222025-06-0110310.47430/ujmr.25103.047Production of Shiga-Toxin by Escherichia coli O157:H7 isolated from Raw Cow Milk obtained within Kaduna Metropolis, NigeriaAbubakar, S. S.0Maiangwa, J.1Idris, S.2Musa, J.3Department of Microbiology Faculty of Life Science, Kaduna State University, Kaduna State, NigeriaDepartment of Microbiology Faculty of Life Science, Kaduna State University, Kaduna State, NigeriaDepartment of Microbiology Faculty of Life Science, Kaduna State University, Kaduna State, NigeriaDepartment of Microbiology Faculty of Life Science, Kaduna State University, Kaduna State, Nigeria Study’s Excerpt: • coli O157:H7 detected in raw cow milk poses a public health risk. • Isolates harbored both stx1 and stx2 virulence genes. • Biofilm formation enhances the pathogenicity of STEC strains. • Essential oils impaired biofilm, with cinnamon showing strongest effect. • Phytochemicals may help reduce STEC virulence in dairy products. Full Abstract: Shiga toxin-producing Escherichia coli O157: H7 (STEC) is a food-borne pathogen that is associated with human illnesses from mild gastroenteritis to severe life-threatening hemolytic uremic syndrome and thrombotic thrombocytopenic purpura. Its pathogenesis can be enhanced by the occurrence of certain virulence genes and the ability to produce biofilm. The present study investigates the spectrum of such pathogenesis on the basis of the occurrence of shiga-toxigenic E. coli O157:H7 in raw cow milk obtained from cattle herds. Furthermore, the inhibitory potential of some selected essential oils against their biofilm formation was determined. Results revealed isolated shiga-toxigenic E. coli O157:H7 harboured the stx1 and stx2 virulence genes. Inhibitory assessments of cinnamon, clove, eucalyptus, and tea tree oils also revealed impairment of biofilm formation at 1%, 2% and 3% concentrations, with cinnamon having a higher inhibitory impact. The presence of E. coli 0157:H7 in raw cow milk suggests that they are not restricted to contaminating raw meat only, but can be a contaminant of dairy products as well. The combined effect of stx1, stx2, and biofilm formation is of public health concern as plasmid transfer among strains could result in antimicrobial resistance. Phytochemicals from plants such as cinnamon, clove, eucalyptus, and tea tree could play vital biofilm inhibitory roles in decreasing the virulence of E. coli 0157:H7. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1169Escherichia coli O157:H7CowMilkShiga-Toxin |
| spellingShingle | Abubakar, S. S. Maiangwa, J. Idris, S. Musa, J. Production of Shiga-Toxin by Escherichia coli O157:H7 isolated from Raw Cow Milk obtained within Kaduna Metropolis, Nigeria UMYU Journal of Microbiology Research Escherichia coli O157:H7 Cow Milk Shiga-Toxin |
| title | Production of Shiga-Toxin by Escherichia coli O157:H7 isolated from Raw Cow Milk obtained within Kaduna Metropolis, Nigeria |
| title_full | Production of Shiga-Toxin by Escherichia coli O157:H7 isolated from Raw Cow Milk obtained within Kaduna Metropolis, Nigeria |
| title_fullStr | Production of Shiga-Toxin by Escherichia coli O157:H7 isolated from Raw Cow Milk obtained within Kaduna Metropolis, Nigeria |
| title_full_unstemmed | Production of Shiga-Toxin by Escherichia coli O157:H7 isolated from Raw Cow Milk obtained within Kaduna Metropolis, Nigeria |
| title_short | Production of Shiga-Toxin by Escherichia coli O157:H7 isolated from Raw Cow Milk obtained within Kaduna Metropolis, Nigeria |
| title_sort | production of shiga toxin by escherichia coli o157 h7 isolated from raw cow milk obtained within kaduna metropolis nigeria |
| topic | Escherichia coli O157:H7 Cow Milk Shiga-Toxin |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/1169 |
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