Intraperitoneal Oxygen/Ozone Treatment Decreases the Formation of Experimental Postsurgical Peritoneal Adhesions and the Levels/Activity of the Local Ubiquitin-Proteasome System
We have investigated whether an oxygen/ozone (95%O2/5%O3) mixture would have potential against the formation of experimental postsurgical peritoneal adhesions. In two groups of rats, one control intraperitoneally injected with 3 mL/rat of O2 and one intraperitoneally injected with oxygen/ozone mixtu...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2011/606718 |
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| author | Clara Di Filippo Annalisa Capuano Barbara Rinaldi Margherita Luongo Biagio Lettieri Francesco Rossi Michele D'Amico |
| author_facet | Clara Di Filippo Annalisa Capuano Barbara Rinaldi Margherita Luongo Biagio Lettieri Francesco Rossi Michele D'Amico |
| author_sort | Clara Di Filippo |
| collection | DOAJ |
| description | We have investigated whether an oxygen/ozone (95%O2/5%O3) mixture would have potential against the formation of experimental postsurgical peritoneal adhesions. In two groups of rats, one control intraperitoneally injected with 3 mL/rat of O2 and one intraperitoneally injected with oxygen/ozone mixture (3 mL/rat equivalent to 300 μg/kg ozone), we induced a midline laparotomy and an enterotomy at the level of the ileum to encourage the formation of peritoneal adhesions. Samples were taken from the parietal peritoneal tissue to assess the formation of adhesions 0 and 10 days after the surgical procedure and to assess the levels of ubiquitin and 20S proteasome. We found decreased formation of postsurgical peritoneal adhesions after treatment of the rats with 300 μg/kg ozone associated with a decreased levels of ubiquitin and 20S proteasome subunit within the adhered tissue. Oxygen/ozone mixture is potentially useful for approaching the post-surgical peritoneal adhesions, and the UPS system is involved in this. |
| format | Article |
| id | doaj-art-a1c8df3296fc4da1bec61dc2eaac15ef |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a1c8df3296fc4da1bec61dc2eaac15ef2025-08-20T02:20:42ZengWileyMediators of Inflammation0962-93511466-18612011-01-01201110.1155/2011/606718606718Intraperitoneal Oxygen/Ozone Treatment Decreases the Formation of Experimental Postsurgical Peritoneal Adhesions and the Levels/Activity of the Local Ubiquitin-Proteasome SystemClara Di Filippo0Annalisa Capuano1Barbara Rinaldi2Margherita Luongo3Biagio Lettieri4Francesco Rossi5Michele D'Amico6Department of Experimental Medicine, Section of Pharmacology “L. Donatelli”, 2nd University of Naples, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology “L. Donatelli”, 2nd University of Naples, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology “L. Donatelli”, 2nd University of Naples, 80138 Naples, ItalyDepartment of Anesthesiological, Surgical, and Emergency Sciences, 2nd University of Naples, 80138 Naples, ItalyDepartment of Anesthesiological, Surgical, and Emergency Sciences, 2nd University of Naples, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology “L. Donatelli”, 2nd University of Naples, 80138 Naples, ItalyDepartment of Experimental Medicine, Section of Pharmacology “L. Donatelli”, 2nd University of Naples, 80138 Naples, ItalyWe have investigated whether an oxygen/ozone (95%O2/5%O3) mixture would have potential against the formation of experimental postsurgical peritoneal adhesions. In two groups of rats, one control intraperitoneally injected with 3 mL/rat of O2 and one intraperitoneally injected with oxygen/ozone mixture (3 mL/rat equivalent to 300 μg/kg ozone), we induced a midline laparotomy and an enterotomy at the level of the ileum to encourage the formation of peritoneal adhesions. Samples were taken from the parietal peritoneal tissue to assess the formation of adhesions 0 and 10 days after the surgical procedure and to assess the levels of ubiquitin and 20S proteasome. We found decreased formation of postsurgical peritoneal adhesions after treatment of the rats with 300 μg/kg ozone associated with a decreased levels of ubiquitin and 20S proteasome subunit within the adhered tissue. Oxygen/ozone mixture is potentially useful for approaching the post-surgical peritoneal adhesions, and the UPS system is involved in this.http://dx.doi.org/10.1155/2011/606718 |
| spellingShingle | Clara Di Filippo Annalisa Capuano Barbara Rinaldi Margherita Luongo Biagio Lettieri Francesco Rossi Michele D'Amico Intraperitoneal Oxygen/Ozone Treatment Decreases the Formation of Experimental Postsurgical Peritoneal Adhesions and the Levels/Activity of the Local Ubiquitin-Proteasome System Mediators of Inflammation |
| title | Intraperitoneal Oxygen/Ozone Treatment Decreases the Formation of Experimental Postsurgical Peritoneal Adhesions and the Levels/Activity of the Local Ubiquitin-Proteasome System |
| title_full | Intraperitoneal Oxygen/Ozone Treatment Decreases the Formation of Experimental Postsurgical Peritoneal Adhesions and the Levels/Activity of the Local Ubiquitin-Proteasome System |
| title_fullStr | Intraperitoneal Oxygen/Ozone Treatment Decreases the Formation of Experimental Postsurgical Peritoneal Adhesions and the Levels/Activity of the Local Ubiquitin-Proteasome System |
| title_full_unstemmed | Intraperitoneal Oxygen/Ozone Treatment Decreases the Formation of Experimental Postsurgical Peritoneal Adhesions and the Levels/Activity of the Local Ubiquitin-Proteasome System |
| title_short | Intraperitoneal Oxygen/Ozone Treatment Decreases the Formation of Experimental Postsurgical Peritoneal Adhesions and the Levels/Activity of the Local Ubiquitin-Proteasome System |
| title_sort | intraperitoneal oxygen ozone treatment decreases the formation of experimental postsurgical peritoneal adhesions and the levels activity of the local ubiquitin proteasome system |
| url | http://dx.doi.org/10.1155/2011/606718 |
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