IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects
Mesenchymal stem cells (MSCs) possess a capacity to enhance cutaneous wound healing that is well characterized. However, the therapeutic effect of MSCs appears to be limited. Modifying MSC target genes to increase necessary biological effects is a promising strategy for wound therapy. Interleukin-10...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/9158016 |
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| _version_ | 1849398516912226304 |
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| author | Shune Xiao Guangtao Huang Zairong Wei Kaiyu Nie Zhiyuan Liu Chengliang Deng Dali Wang |
| author_facet | Shune Xiao Guangtao Huang Zairong Wei Kaiyu Nie Zhiyuan Liu Chengliang Deng Dali Wang |
| author_sort | Shune Xiao |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) possess a capacity to enhance cutaneous wound healing that is well characterized. However, the therapeutic effect of MSCs appears to be limited. Modifying MSC target genes to increase necessary biological effects is a promising strategy for wound therapy. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that has a therapeutic effect on wound healing. In this study, we modified human amniotic mesenchymal stem cells (hAMSCs) using recombinant lentiviral vectors for expressing IL-10 and evaluated the therapeutic effects of hAMSCs-IL-10 in wound healing. We elucidated the mechanisms underlying the effects. We found that promoting wound healing was maintained by synergistic effects of hAMSCs and IL-10. hAMSCs-IL-10 showed stronger biological effects in accelerating wound closure, enhancing angiogenesis, modulating inflammation, and regulating extracellular matrix remodeling than hAMSCs. hAMSCs-IL-10 would be better at promoting wound healing and improving healing quality. These data may provide a theoretical foundation for clinical administration of hAMSCs-IL-10 in cutaneous wound healing and skin regeneration. |
| format | Article |
| id | doaj-art-a1c2b7a0ebee40ac8d65b10c2faa7392 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a1c2b7a0ebee40ac8d65b10c2faa73922025-08-20T03:38:35ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/91580169158016IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic EffectsShune Xiao0Guangtao Huang1Zairong Wei2Kaiyu Nie3Zhiyuan Liu4Chengliang Deng5Dali Wang6Department of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaDepartment of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, ChinaMesenchymal stem cells (MSCs) possess a capacity to enhance cutaneous wound healing that is well characterized. However, the therapeutic effect of MSCs appears to be limited. Modifying MSC target genes to increase necessary biological effects is a promising strategy for wound therapy. Interleukin-10 (IL-10) is an anti-inflammatory cytokine that has a therapeutic effect on wound healing. In this study, we modified human amniotic mesenchymal stem cells (hAMSCs) using recombinant lentiviral vectors for expressing IL-10 and evaluated the therapeutic effects of hAMSCs-IL-10 in wound healing. We elucidated the mechanisms underlying the effects. We found that promoting wound healing was maintained by synergistic effects of hAMSCs and IL-10. hAMSCs-IL-10 showed stronger biological effects in accelerating wound closure, enhancing angiogenesis, modulating inflammation, and regulating extracellular matrix remodeling than hAMSCs. hAMSCs-IL-10 would be better at promoting wound healing and improving healing quality. These data may provide a theoretical foundation for clinical administration of hAMSCs-IL-10 in cutaneous wound healing and skin regeneration.http://dx.doi.org/10.1155/2019/9158016 |
| spellingShingle | Shune Xiao Guangtao Huang Zairong Wei Kaiyu Nie Zhiyuan Liu Chengliang Deng Dali Wang IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects Stem Cells International |
| title | IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects |
| title_full | IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects |
| title_fullStr | IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects |
| title_full_unstemmed | IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects |
| title_short | IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects |
| title_sort | il 10 gene modified human amniotic mesenchymal stem cells augment regenerative wound healing by multiple synergistic effects |
| url | http://dx.doi.org/10.1155/2019/9158016 |
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