Oral Administration of GnRH and Domperidone via Gel Feed and Their Effect on Reproductive Performance of <i>Devario devario</i> (Bengal Danio), an Ornamental Fish
This study investigated the effects of dietary Gonadotropin-releasing hormone (GnRH) and domperidone on the reproductive performance of <i>Devario devario</i> during a 40-day trial. Five treatment groups received varying doses of GnRH (100, 50, 25, 12.5 µg/kg body weight) in combination...
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2025-07-01
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| author | Suparna Deb Pradyut Biswas Soibam Khogen Singh Gusheinzed Waikhom Reshmi Debbarma Shubham Kashyap Jham Lal Khusbu Samal Supratim Malla Nitesh Kumar Yadav Ng. Chinglembi Devi Pronob Das N. Sureshchandra Singh G. Deepak Reddy Surajkumar Irungbam |
| author_facet | Suparna Deb Pradyut Biswas Soibam Khogen Singh Gusheinzed Waikhom Reshmi Debbarma Shubham Kashyap Jham Lal Khusbu Samal Supratim Malla Nitesh Kumar Yadav Ng. Chinglembi Devi Pronob Das N. Sureshchandra Singh G. Deepak Reddy Surajkumar Irungbam |
| author_sort | Suparna Deb |
| collection | DOAJ |
| description | This study investigated the effects of dietary Gonadotropin-releasing hormone (GnRH) and domperidone on the reproductive performance of <i>Devario devario</i> during a 40-day trial. Five treatment groups received varying doses of GnRH (100, 50, 25, 12.5 µg/kg body weight) in combination with domperidone (50, 25, 12.5, 6.25 mg/kg body weight), embossed in a gel-based diet alongside a control group without the exogenous hormones. Reproductive performance was examined by measuring the gonadosomatic index, fecundity, reproductive hormone levels, and histological features of the gonads, blood parameters, and antioxidant enzyme activity. The T1 group (100 µg GnRH + 50 mg domperidone) exhibited the highest GSI in both sexes. The histological analysis of testes from T1, T2 (50 µg GnRH + 25 mg domperidone), and T3 (25 µg GnRH + 12.5 mg domperidone) groups revealed an increased presence of late-stage spermatids and spermatozoa. In females, the T2 group produced the highest proportion of advanced-stage oocytes and demonstrated the greatest absolute fecundity (1300 ± 23 eggs). However, the control group showed the highest fertilization and hatching rates. Testosterone levels were significantly elevated in the T3 group, while vitellogenin levels increased in the T1 and T2 groups. Antioxidant enzyme activity varied, with the T1 group displaying higher superoxide dismutase activity in gills and liver, and the T2 group showing increased SOD activity in muscle and brain. Improvements in haematological parameters were observed across all treatments. These results suggest that an optimal dose of 50 µg GnRH + 25 mg domperidone can enhance reproductive performance in <i>D. devario</i>. |
| format | Article |
| id | doaj-art-a1bdfb08074d4e72bd574f7258776864 |
| institution | Kabale University |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Gels |
| spelling | doaj-art-a1bdfb08074d4e72bd574f72587768642025-08-20T03:35:37ZengMDPI AGGels2310-28612025-07-0111755410.3390/gels11070554Oral Administration of GnRH and Domperidone via Gel Feed and Their Effect on Reproductive Performance of <i>Devario devario</i> (Bengal Danio), an Ornamental FishSuparna Deb0Pradyut Biswas1Soibam Khogen Singh2Gusheinzed Waikhom3Reshmi Debbarma4Shubham Kashyap5Jham Lal6Khusbu Samal7Supratim Malla8Nitesh Kumar Yadav9Ng. Chinglembi Devi10Pronob Das11N. Sureshchandra Singh12G. Deepak Reddy13Surajkumar Irungbam14College of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaICAR-Central Inland Fisheries Research Institute, Regional Centre, Guwahati 781006, Assam, IndiaKrishi Vigyan Kendra, Ukhrul, ICAR Research Complex for NEH Region, Imphal 795142, Manipur, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaCollege of Fisheries, Central Agricultural University, Lembucherra, Agartala 799210, Tripura, IndiaThis study investigated the effects of dietary Gonadotropin-releasing hormone (GnRH) and domperidone on the reproductive performance of <i>Devario devario</i> during a 40-day trial. Five treatment groups received varying doses of GnRH (100, 50, 25, 12.5 µg/kg body weight) in combination with domperidone (50, 25, 12.5, 6.25 mg/kg body weight), embossed in a gel-based diet alongside a control group without the exogenous hormones. Reproductive performance was examined by measuring the gonadosomatic index, fecundity, reproductive hormone levels, and histological features of the gonads, blood parameters, and antioxidant enzyme activity. The T1 group (100 µg GnRH + 50 mg domperidone) exhibited the highest GSI in both sexes. The histological analysis of testes from T1, T2 (50 µg GnRH + 25 mg domperidone), and T3 (25 µg GnRH + 12.5 mg domperidone) groups revealed an increased presence of late-stage spermatids and spermatozoa. In females, the T2 group produced the highest proportion of advanced-stage oocytes and demonstrated the greatest absolute fecundity (1300 ± 23 eggs). However, the control group showed the highest fertilization and hatching rates. Testosterone levels were significantly elevated in the T3 group, while vitellogenin levels increased in the T1 and T2 groups. Antioxidant enzyme activity varied, with the T1 group displaying higher superoxide dismutase activity in gills and liver, and the T2 group showing increased SOD activity in muscle and brain. Improvements in haematological parameters were observed across all treatments. These results suggest that an optimal dose of 50 µg GnRH + 25 mg domperidone can enhance reproductive performance in <i>D. devario</i>.https://www.mdpi.com/2310-2861/11/7/554gonadosomatic indexfecundityGSI<i>Devario devario</i> |
| spellingShingle | Suparna Deb Pradyut Biswas Soibam Khogen Singh Gusheinzed Waikhom Reshmi Debbarma Shubham Kashyap Jham Lal Khusbu Samal Supratim Malla Nitesh Kumar Yadav Ng. Chinglembi Devi Pronob Das N. Sureshchandra Singh G. Deepak Reddy Surajkumar Irungbam Oral Administration of GnRH and Domperidone via Gel Feed and Their Effect on Reproductive Performance of <i>Devario devario</i> (Bengal Danio), an Ornamental Fish Gels gonadosomatic index fecundity GSI <i>Devario devario</i> |
| title | Oral Administration of GnRH and Domperidone via Gel Feed and Their Effect on Reproductive Performance of <i>Devario devario</i> (Bengal Danio), an Ornamental Fish |
| title_full | Oral Administration of GnRH and Domperidone via Gel Feed and Their Effect on Reproductive Performance of <i>Devario devario</i> (Bengal Danio), an Ornamental Fish |
| title_fullStr | Oral Administration of GnRH and Domperidone via Gel Feed and Their Effect on Reproductive Performance of <i>Devario devario</i> (Bengal Danio), an Ornamental Fish |
| title_full_unstemmed | Oral Administration of GnRH and Domperidone via Gel Feed and Their Effect on Reproductive Performance of <i>Devario devario</i> (Bengal Danio), an Ornamental Fish |
| title_short | Oral Administration of GnRH and Domperidone via Gel Feed and Their Effect on Reproductive Performance of <i>Devario devario</i> (Bengal Danio), an Ornamental Fish |
| title_sort | oral administration of gnrh and domperidone via gel feed and their effect on reproductive performance of i devario devario i bengal danio an ornamental fish |
| topic | gonadosomatic index fecundity GSI <i>Devario devario</i> |
| url | https://www.mdpi.com/2310-2861/11/7/554 |
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