ANTIMICROBIAL ACTIVITY, ALONE AND IN CONJUNCTION WITH CEFUROXIME
Introduction: Occurrence of multidrug resistant microorganism is the result of irrational use of antibiotics. Recently essential oil, an important constituent of therapeutic and scented plant has been explored for their antimicrobial activity. Objectives: In this study, antibacterial activities of...
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Superior University Lahore, Pakistan
2023-03-01
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| Series: | International Journal of Pharmacy & Integrated Health Sciences |
| Online Access: | https://ijpihs.pk/index.php/IJPIHS/article/view/55 |
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| author | amna naz Sadia Haider Syed Muhammad Ali |
| author_facet | amna naz Sadia Haider Syed Muhammad Ali |
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Introduction: Occurrence of multidrug resistant microorganism is the result of irrational use of antibiotics. Recently essential oil, an important constituent of therapeutic and scented plant has been explored for their antimicrobial activity. Objectives: In this study, antibacterial activities of rosemary essential oil (REO) alone and also in conjunction with beta lactam antibiotic cefuroxime (CXM) were assessed. The purity of REO was checked by gas chromatography / mass spectrophotometry method (GC-MS). Methodology: By agar well diffusion method for REO and its diluents with dimethyl sulfoxide (DMSO) and tween 80, the zone of inhibition (ZOI) against four Gram positive bacterial strains of Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and Staphylococcus epidermidis and three Gram negative bacterial strains of Pseudomonas aeruginosa (1:2,1:10, 1:20, 1:40 and 1:50). E. coli as bacterial and DMSO as solvent were used as a control. Results: Antimicrobial activity was found to be highest against Staphylococcus aureus with ZOI 17.56mm and the lowest against Pseudomonas aeruginosa with ZOI 5.53 mm. The combination of REO and CXM indicated synergistic effects against E. coli, Staphylococcus aureus, Klebsiella pneumonia and Proteus mirabilis and antagonistic effect against Pseudomonas aeruginosa and indifferent effects against Bacillus subtilis and bacillus epidermidis based on fractional inhibitory concentration (FIC) values. The t test was applied to FIC values that showed significant p value (< 0.5). Conclusion: In conclusion, the antibacterial activity of the REO and CXM was enhanced when administered in combination, with the exception of Pseudomonas aeruginosa. In future this study may lead the way to the development of new drug combinations and dosage forms for increased antimicrobial and a reduced resistance against common pathogens.
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| format | Article |
| id | doaj-art-a1b5c8534f3e408a83c08832f9308fe6 |
| institution | Kabale University |
| issn | 2958-1656 2789-2840 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Superior University Lahore, Pakistan |
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| series | International Journal of Pharmacy & Integrated Health Sciences |
| spelling | doaj-art-a1b5c8534f3e408a83c08832f9308fe62025-08-20T03:31:10ZengSuperior University Lahore, PakistanInternational Journal of Pharmacy & Integrated Health Sciences2958-16562789-28402023-03-014110.56536/ijpihs.v4i1.55ANTIMICROBIAL ACTIVITY, ALONE AND IN CONJUNCTION WITH CEFUROXIMEamna naz0Sadia Haider1Syed Muhammad Ali2Lahore College for Women UniversityLahore College for Women University, Lahore, PakistanUniversity College of Pharmacy, University of the Punjab Lahore Pakistan. Introduction: Occurrence of multidrug resistant microorganism is the result of irrational use of antibiotics. Recently essential oil, an important constituent of therapeutic and scented plant has been explored for their antimicrobial activity. Objectives: In this study, antibacterial activities of rosemary essential oil (REO) alone and also in conjunction with beta lactam antibiotic cefuroxime (CXM) were assessed. The purity of REO was checked by gas chromatography / mass spectrophotometry method (GC-MS). Methodology: By agar well diffusion method for REO and its diluents with dimethyl sulfoxide (DMSO) and tween 80, the zone of inhibition (ZOI) against four Gram positive bacterial strains of Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and Staphylococcus epidermidis and three Gram negative bacterial strains of Pseudomonas aeruginosa (1:2,1:10, 1:20, 1:40 and 1:50). E. coli as bacterial and DMSO as solvent were used as a control. Results: Antimicrobial activity was found to be highest against Staphylococcus aureus with ZOI 17.56mm and the lowest against Pseudomonas aeruginosa with ZOI 5.53 mm. The combination of REO and CXM indicated synergistic effects against E. coli, Staphylococcus aureus, Klebsiella pneumonia and Proteus mirabilis and antagonistic effect against Pseudomonas aeruginosa and indifferent effects against Bacillus subtilis and bacillus epidermidis based on fractional inhibitory concentration (FIC) values. The t test was applied to FIC values that showed significant p value (< 0.5). Conclusion: In conclusion, the antibacterial activity of the REO and CXM was enhanced when administered in combination, with the exception of Pseudomonas aeruginosa. In future this study may lead the way to the development of new drug combinations and dosage forms for increased antimicrobial and a reduced resistance against common pathogens. https://ijpihs.pk/index.php/IJPIHS/article/view/55 |
| spellingShingle | amna naz Sadia Haider Syed Muhammad Ali ANTIMICROBIAL ACTIVITY, ALONE AND IN CONJUNCTION WITH CEFUROXIME International Journal of Pharmacy & Integrated Health Sciences |
| title | ANTIMICROBIAL ACTIVITY, ALONE AND IN CONJUNCTION WITH CEFUROXIME |
| title_full | ANTIMICROBIAL ACTIVITY, ALONE AND IN CONJUNCTION WITH CEFUROXIME |
| title_fullStr | ANTIMICROBIAL ACTIVITY, ALONE AND IN CONJUNCTION WITH CEFUROXIME |
| title_full_unstemmed | ANTIMICROBIAL ACTIVITY, ALONE AND IN CONJUNCTION WITH CEFUROXIME |
| title_short | ANTIMICROBIAL ACTIVITY, ALONE AND IN CONJUNCTION WITH CEFUROXIME |
| title_sort | antimicrobial activity alone and in conjunction with cefuroxime |
| url | https://ijpihs.pk/index.php/IJPIHS/article/view/55 |
| work_keys_str_mv | AT amnanaz antimicrobialactivityaloneandinconjunctionwithcefuroxime AT sadiahaider antimicrobialactivityaloneandinconjunctionwithcefuroxime AT syedmuhammadali antimicrobialactivityaloneandinconjunctionwithcefuroxime |