The potential of urinary miR-200c-3p as a biomarker of fluorosis in rats
Fluorine is a strong oxidizing element and excessive intake can have harmful effects, particularly on the body's calcified tissues. Recent studies have demonstrated a link between miRNA and fluorosis. This study aimed to evaluate the time-dose-effect relationship of miR-200c-3p in plasma, urine...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324017202 |
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| author | Ailin Li Jian Wang Xinyue Meng Xu Ma Ying Liu Hanying Li Zhe Mo Rui Zhang Lei Wu Shirui Yan Paula Fiorella Chacon Campoverde Gazala Zafar Yongzheng Ma Wenjun Que Zhe Li Tuo Wang Korto Fatti Tarnue Junrui Pei |
| author_facet | Ailin Li Jian Wang Xinyue Meng Xu Ma Ying Liu Hanying Li Zhe Mo Rui Zhang Lei Wu Shirui Yan Paula Fiorella Chacon Campoverde Gazala Zafar Yongzheng Ma Wenjun Que Zhe Li Tuo Wang Korto Fatti Tarnue Junrui Pei |
| author_sort | Ailin Li |
| collection | DOAJ |
| description | Fluorine is a strong oxidizing element and excessive intake can have harmful effects, particularly on the body's calcified tissues. Recent studies have demonstrated a link between miRNA and fluorosis. This study aimed to evaluate the time-dose-effect relationship of miR-200c-3p in plasma, urine and cartilage of rats with drinking water fluorosis, and to explore its potential as a biomarker. Analyses were conducted using Generalised linear models, Restricted cubic spline, Spearman correlation analysis, and Receiver operating characteristic curve (ROC). Results indicated that both fluoride exposure time and dose had significantly affected on urinary and cartilage miR-200c-3p expression in rats, while plasma miR-200c-3p expression was only influenced by fluoride exposure time. Restricted cubic spline plots revealed that urinary miR-200c-3p was non-linearly and positively correlated with serum fluoride, urinary fluoride, dental fluorosis, and Mankin score groups, and also linearly and positively correlated with cartilage fluoride. Regression analysis showed that for each unit increase in urinary miR-200c-3p, the likelihood of dental fluorosis increased by 1.300 times, and in the Mankin score groups, the likelihood increased by 1.251 times. The ROC curves demonstrated that urinary miR-200c-3p had high sensitivity and specificity in diagnosing dental and skeletal fluorosis. Blood and cartilage miR-200c-3p showed weaker diagnostic efficacy. In summary, fluoride has different effects on the expression levels of miRNA-200c in various biological samples of rats, and miRNAs in urine demonstrate potential as biomarkers for fluorosis. |
| format | Article |
| id | doaj-art-a1b4d07c6e3c42458290899788f77a9d |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-a1b4d07c6e3c42458290899788f77a9d2025-01-23T05:25:57ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117644The potential of urinary miR-200c-3p as a biomarker of fluorosis in ratsAilin Li0Jian Wang1Xinyue Meng2Xu Ma3Ying Liu4Hanying Li5Zhe Mo6Rui Zhang7Lei Wu8Shirui Yan9Paula Fiorella Chacon Campoverde10Gazala Zafar11Yongzheng Ma12Wenjun Que13Zhe Li14Tuo Wang15Korto Fatti Tarnue16Junrui Pei17Key Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, China; Department of Environmental Health, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, ChinaKey Lab of Etiology and Epidemiology, Education Bureau of Heilongjiang Province & Ministry of Health (23618504), Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang Province 150081, China; Corresponding author.Fluorine is a strong oxidizing element and excessive intake can have harmful effects, particularly on the body's calcified tissues. Recent studies have demonstrated a link between miRNA and fluorosis. This study aimed to evaluate the time-dose-effect relationship of miR-200c-3p in plasma, urine and cartilage of rats with drinking water fluorosis, and to explore its potential as a biomarker. Analyses were conducted using Generalised linear models, Restricted cubic spline, Spearman correlation analysis, and Receiver operating characteristic curve (ROC). Results indicated that both fluoride exposure time and dose had significantly affected on urinary and cartilage miR-200c-3p expression in rats, while plasma miR-200c-3p expression was only influenced by fluoride exposure time. Restricted cubic spline plots revealed that urinary miR-200c-3p was non-linearly and positively correlated with serum fluoride, urinary fluoride, dental fluorosis, and Mankin score groups, and also linearly and positively correlated with cartilage fluoride. Regression analysis showed that for each unit increase in urinary miR-200c-3p, the likelihood of dental fluorosis increased by 1.300 times, and in the Mankin score groups, the likelihood increased by 1.251 times. The ROC curves demonstrated that urinary miR-200c-3p had high sensitivity and specificity in diagnosing dental and skeletal fluorosis. Blood and cartilage miR-200c-3p showed weaker diagnostic efficacy. In summary, fluoride has different effects on the expression levels of miRNA-200c in various biological samples of rats, and miRNAs in urine demonstrate potential as biomarkers for fluorosis.http://www.sciencedirect.com/science/article/pii/S0147651324017202FluorideFluorosisMiR-200c-3pBiomarkersRestricted cubic splineReceiver operating characteristic curve |
| spellingShingle | Ailin Li Jian Wang Xinyue Meng Xu Ma Ying Liu Hanying Li Zhe Mo Rui Zhang Lei Wu Shirui Yan Paula Fiorella Chacon Campoverde Gazala Zafar Yongzheng Ma Wenjun Que Zhe Li Tuo Wang Korto Fatti Tarnue Junrui Pei The potential of urinary miR-200c-3p as a biomarker of fluorosis in rats Ecotoxicology and Environmental Safety Fluoride Fluorosis MiR-200c-3p Biomarkers Restricted cubic spline Receiver operating characteristic curve |
| title | The potential of urinary miR-200c-3p as a biomarker of fluorosis in rats |
| title_full | The potential of urinary miR-200c-3p as a biomarker of fluorosis in rats |
| title_fullStr | The potential of urinary miR-200c-3p as a biomarker of fluorosis in rats |
| title_full_unstemmed | The potential of urinary miR-200c-3p as a biomarker of fluorosis in rats |
| title_short | The potential of urinary miR-200c-3p as a biomarker of fluorosis in rats |
| title_sort | potential of urinary mir 200c 3p as a biomarker of fluorosis in rats |
| topic | Fluoride Fluorosis MiR-200c-3p Biomarkers Restricted cubic spline Receiver operating characteristic curve |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324017202 |
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