MicroRNA‐1307‐3p contributes to breast cancer progression through PRM2
Abstract Background Despite advances in screening and therapy, breast cancer (BC) remains the predominant cancer in women globally. Dysregulation of microRNAs (miRNAs) is pivotal in carcinogenesis across various cancers, including BC. Evidence indicates that miR‐1307‐3p is upregulated in BC tumors,...
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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | Thoracic Cancer |
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| Online Access: | https://doi.org/10.1111/1759-7714.15460 |
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| author | José Roberto Estupiñan‐Jiménez Valeria Villarreal‐García Vianey Gonzalez‐Villasana Pablo E. Vivas‐Mejia Jose Manuel Vazquez‐Guillen Patricio Adrián Zapata‐Morin Marienid Flores‐Colón Claudia Altamirano‐Torres Ezequiel Viveros‐Valdez Cristina Ivan Mohammed H. Rashed Recep Bayraktar Cristina Rodríguez‐Padilla Gabriel Lopez‐Berestein Diana Resendez‐Perez |
| author_facet | José Roberto Estupiñan‐Jiménez Valeria Villarreal‐García Vianey Gonzalez‐Villasana Pablo E. Vivas‐Mejia Jose Manuel Vazquez‐Guillen Patricio Adrián Zapata‐Morin Marienid Flores‐Colón Claudia Altamirano‐Torres Ezequiel Viveros‐Valdez Cristina Ivan Mohammed H. Rashed Recep Bayraktar Cristina Rodríguez‐Padilla Gabriel Lopez‐Berestein Diana Resendez‐Perez |
| author_sort | José Roberto Estupiñan‐Jiménez |
| collection | DOAJ |
| description | Abstract Background Despite advances in screening and therapy, breast cancer (BC) remains the predominant cancer in women globally. Dysregulation of microRNAs (miRNAs) is pivotal in carcinogenesis across various cancers, including BC. Evidence indicates that miR‐1307‐3p is upregulated in BC tumors, yet its target genes are not fully elucidated. This study aimed to explore how miR‐1307‐3p regulates BC proliferation, migration, invasion, and angiogenesis and to identify potential target genes. Methods Basal miR‐1307‐3p levels were quantified in BC cell lines MDA‐MB‐231 and MCF‐7, as well as MCF‐10A using quantitative real‐time reverse transcription‐PCR (RT‐qPCR). The impact of miR‐1307‐3p inhibition on BC cell proliferation, migration, invasion, and angiogenesis was assessed. Nine miRNA‐target prediction databases identified potential miR‐1307‐3p targets. Target expression was validated using RT‐qPCR, Western blot, and dual‐luciferase reporter assays. MiR‐1307‐3p was overexpressed in MDA‐MB‐231 and MCF‐7 compared to MCF‐10A. Results Inhibiting miR‐1307‐3p significantly reduced BC cell proliferation, migration, invasion, and angiogenesis. Bioinformatics analysis identified 17 potential miR‐1307‐3p targets, with protamine 2 (PRM2) overexpression confirmed via Western blot and dual‐luciferase assays. Conclusion MiR‐1307‐3p overexpression in BC promotes proliferation, migration, invasion, and angiogenesis. PRM2 emerges as a novel miR‐1307‐3p target in BC. |
| format | Article |
| id | doaj-art-a1b3346bdbed4eaaa2d23f4f940cb712 |
| institution | OA Journals |
| issn | 1759-7706 1759-7714 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj-art-a1b3346bdbed4eaaa2d23f4f940cb7122025-08-20T02:13:59ZengWileyThoracic Cancer1759-77061759-77142024-11-0115322298230810.1111/1759-7714.15460MicroRNA‐1307‐3p contributes to breast cancer progression through PRM2José Roberto Estupiñan‐Jiménez0Valeria Villarreal‐García1Vianey Gonzalez‐Villasana2Pablo E. Vivas‐Mejia3Jose Manuel Vazquez‐Guillen4Patricio Adrián Zapata‐Morin5Marienid Flores‐Colón6Claudia Altamirano‐Torres7Ezequiel Viveros‐Valdez8Cristina Ivan9Mohammed H. Rashed10Recep Bayraktar11Cristina Rodríguez‐Padilla12Gabriel Lopez‐Berestein13Diana Resendez‐Perez14Departmento de Biología Celular y Genética, Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza MexicoDepartmento de Biología Celular y Genética, Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza MexicoDepartmento de Biología Celular y Genética, Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza MexicoDepartment of Biochemistry, Medical Sciences Campus University of Puerto Rico San Juan Puerto RicoLaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza MexicoLaboratorio de Micología y Fitopatología, Unidad de Manipulación Genética, Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza MexicoDepartment of Biochemistry, Medical Sciences Campus University of Puerto Rico San Juan Puerto RicoDepartmento de Biología Celular y Genética, Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza MexicoDepartamento de Química, Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza MexicoDepartment of Experimental Therapeutics The University of Texas MD Anderson Cancer Center Houston Texas USAClinical Pharmacy Department, Faculty of Pharmacy (Boys) Al‐Azhar University Cairo EgyptDepartment of Translational Molecular Pathology The University of Texas MD Anderson Cancer Center Houston Texas USALaboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza MexicoDepartment of Experimental Therapeutics The University of Texas MD Anderson Cancer Center Houston Texas USADepartmento de Biología Celular y Genética, Facultad de Ciencias Biológicas Universidad Autónoma de Nuevo León San Nicolás de los Garza MexicoAbstract Background Despite advances in screening and therapy, breast cancer (BC) remains the predominant cancer in women globally. Dysregulation of microRNAs (miRNAs) is pivotal in carcinogenesis across various cancers, including BC. Evidence indicates that miR‐1307‐3p is upregulated in BC tumors, yet its target genes are not fully elucidated. This study aimed to explore how miR‐1307‐3p regulates BC proliferation, migration, invasion, and angiogenesis and to identify potential target genes. Methods Basal miR‐1307‐3p levels were quantified in BC cell lines MDA‐MB‐231 and MCF‐7, as well as MCF‐10A using quantitative real‐time reverse transcription‐PCR (RT‐qPCR). The impact of miR‐1307‐3p inhibition on BC cell proliferation, migration, invasion, and angiogenesis was assessed. Nine miRNA‐target prediction databases identified potential miR‐1307‐3p targets. Target expression was validated using RT‐qPCR, Western blot, and dual‐luciferase reporter assays. MiR‐1307‐3p was overexpressed in MDA‐MB‐231 and MCF‐7 compared to MCF‐10A. Results Inhibiting miR‐1307‐3p significantly reduced BC cell proliferation, migration, invasion, and angiogenesis. Bioinformatics analysis identified 17 potential miR‐1307‐3p targets, with protamine 2 (PRM2) overexpression confirmed via Western blot and dual‐luciferase assays. Conclusion MiR‐1307‐3p overexpression in BC promotes proliferation, migration, invasion, and angiogenesis. PRM2 emerges as a novel miR‐1307‐3p target in BC.https://doi.org/10.1111/1759-7714.15460breast cancercancer progressionmicroRNAsmiR‐1307‐3pprotamine 2 |
| spellingShingle | José Roberto Estupiñan‐Jiménez Valeria Villarreal‐García Vianey Gonzalez‐Villasana Pablo E. Vivas‐Mejia Jose Manuel Vazquez‐Guillen Patricio Adrián Zapata‐Morin Marienid Flores‐Colón Claudia Altamirano‐Torres Ezequiel Viveros‐Valdez Cristina Ivan Mohammed H. Rashed Recep Bayraktar Cristina Rodríguez‐Padilla Gabriel Lopez‐Berestein Diana Resendez‐Perez MicroRNA‐1307‐3p contributes to breast cancer progression through PRM2 Thoracic Cancer breast cancer cancer progression microRNAs miR‐1307‐3p protamine 2 |
| title | MicroRNA‐1307‐3p contributes to breast cancer progression through PRM2 |
| title_full | MicroRNA‐1307‐3p contributes to breast cancer progression through PRM2 |
| title_fullStr | MicroRNA‐1307‐3p contributes to breast cancer progression through PRM2 |
| title_full_unstemmed | MicroRNA‐1307‐3p contributes to breast cancer progression through PRM2 |
| title_short | MicroRNA‐1307‐3p contributes to breast cancer progression through PRM2 |
| title_sort | microrna 1307 3p contributes to breast cancer progression through prm2 |
| topic | breast cancer cancer progression microRNAs miR‐1307‐3p protamine 2 |
| url | https://doi.org/10.1111/1759-7714.15460 |
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