Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors
To date, great attention is paid to sex and age differences in the therapeutic effectiveness of drugs, including those that impact the immune system. Bacteriophage-derived dsRNA is the main component of the medicinal product Larifan, which exhibits interferonogenic activity. This study aimed to esti...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2024-10-01
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| Series: | The Ukrainian Biochemical Journal |
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| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2024/10/Dovhyi_96_5.pdf |
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| author | R. Dovhyi M. Rudyk T. Serhiichuk Yu. Yumyna A. Dvukhriadkina K. Ostrovska D. Pjanova L. Skivka |
| author_facet | R. Dovhyi M. Rudyk T. Serhiichuk Yu. Yumyna A. Dvukhriadkina K. Ostrovska D. Pjanova L. Skivka |
| author_sort | R. Dovhyi |
| collection | DOAJ |
| description | To date, great attention is paid to sex and age differences in the therapeutic effectiveness of drugs, including those that impact the immune system. Bacteriophage-derived dsRNA is the main component of the medicinal product Larifan, which exhibits interferonogenic activity. This study aimed to estimate the effect of Larifan on the activation status of human peripheral blood monocytes collected from donors of different ages and sex. Blood samples were obtained from the healthy volunteers, divided into 4 groups: young men and young women aged from 20 to 39 years, aged men and aged women from 54 to 69 years old. EDTA-anticoagulated blood samples were exposed to 200 μg/ml Larifan for 30 min, cells were washed and treated to study phagocytic index, ROS generation and expression of phenotypic markers. Only live monocytes selected by flow cytometry were included in the analysis. It was shown that monocytes from young as well as from aged females turned out to be quite inert to the treatment with Larifan. Monocytes from young males after the treatment demonstrated a minor decrease in phagocytic activity and significant down-regulation of ROS generation. Monocytes from aged adults showed clear sex-based differences in the basal cell phenotype. Thus, compared to monocytes from women, the monocytes from men over 50 after the treatment with Larifan showed decreased phagocytic activity and CD86 expression along with increased CD206 expression. Taken together, these results indicate the need for further studies of Larifan focused on developing personalized treatment depending on the age and sex of an individual. |
| format | Article |
| id | doaj-art-a1b075dca8624b5196ef0060fdc0b9ac |
| institution | OA Journals |
| issn | 2409-4943 2413-5003 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj-art-a1b075dca8624b5196ef0060fdc0b9ac2025-08-20T02:31:27ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032024-10-01965445410.15407/ubj96.05.044Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donorsR. Dovhyi 0https://orcid.org/0000-0003-1189-4479M. Rudyk1https://orcid.org/0000-0003-1252-885XT. Serhiichuk2https://orcid.org/0000-0002-6351-2412Yu. Yumyna3A. Dvukhriadkina4K. Ostrovska5https://orcid.org/0009-0004-9170-0842D. Pjanova6https://orcid.org/0000-0002-3993-7939L. Skivka7ESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineLatvian Biomedical Research and Study Centre, Riga, LatviaESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineTo date, great attention is paid to sex and age differences in the therapeutic effectiveness of drugs, including those that impact the immune system. Bacteriophage-derived dsRNA is the main component of the medicinal product Larifan, which exhibits interferonogenic activity. This study aimed to estimate the effect of Larifan on the activation status of human peripheral blood monocytes collected from donors of different ages and sex. Blood samples were obtained from the healthy volunteers, divided into 4 groups: young men and young women aged from 20 to 39 years, aged men and aged women from 54 to 69 years old. EDTA-anticoagulated blood samples were exposed to 200 μg/ml Larifan for 30 min, cells were washed and treated to study phagocytic index, ROS generation and expression of phenotypic markers. Only live monocytes selected by flow cytometry were included in the analysis. It was shown that monocytes from young as well as from aged females turned out to be quite inert to the treatment with Larifan. Monocytes from young males after the treatment demonstrated a minor decrease in phagocytic activity and significant down-regulation of ROS generation. Monocytes from aged adults showed clear sex-based differences in the basal cell phenotype. Thus, compared to monocytes from women, the monocytes from men over 50 after the treatment with Larifan showed decreased phagocytic activity and CD86 expression along with increased CD206 expression. Taken together, these results indicate the need for further studies of Larifan focused on developing personalized treatment depending on the age and sex of an individual.http://ukrbiochemjournal.org/wp-content/uploads/2024/10/Dovhyi_96_5.pdfdouble-stranded rnalarifanmonocytesphagocytosisreactive oxygen speciessex and age differences |
| spellingShingle | R. Dovhyi M. Rudyk T. Serhiichuk Yu. Yumyna A. Dvukhriadkina K. Ostrovska D. Pjanova L. Skivka Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors The Ukrainian Biochemical Journal double-stranded rna larifan monocytes phagocytosis reactive oxygen species sex and age differences |
| title | Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors |
| title_full | Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors |
| title_fullStr | Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors |
| title_full_unstemmed | Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors |
| title_short | Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors |
| title_sort | bacteriophage derived double stranded rna larifan exerts variable effects on human blood monocytes depending on age and sex of donors |
| topic | double-stranded rna larifan monocytes phagocytosis reactive oxygen species sex and age differences |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2024/10/Dovhyi_96_5.pdf |
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