Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategies
Transglutaminase (TGase) has been widely applied in the food industry. However, achieving high-yield TGase production remains a challenge, limiting its broader industrial application. In this study, a high-yield strain with stable genetic traits was obtained through UV-ARTP combined mutagenesis, ach...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1525673/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832539917008240640 |
|---|---|
| author | Huanan Chang Ziyu Zheng Hao Li Yanqiu Xu Gengyao Zhen Yao Zhang Xidong Ren Xidong Ren Xinli Liu Xinli Liu Deqiang Zhu Deqiang Zhu |
| author_facet | Huanan Chang Ziyu Zheng Hao Li Yanqiu Xu Gengyao Zhen Yao Zhang Xidong Ren Xidong Ren Xinli Liu Xinli Liu Deqiang Zhu Deqiang Zhu |
| author_sort | Huanan Chang |
| collection | DOAJ |
| description | Transglutaminase (TGase) has been widely applied in the food industry. However, achieving high-yield TGase production remains a challenge, limiting its broader industrial application. In this study, a high-yield strain with stable genetic traits was obtained through UV-ARTP combined mutagenesis, achieving a maximum TGase activity of 13.77 U/mL, representing a 92.43% increase. Using this strain as a forward mutation gene pool, comparative genomic research identified 95 mutated genes, which were mostly due to base substitutions that led to changes in codon usage preference. Transcriptomic analysis revealed significant expression changes in 470 genes, with 232 upregulated and 238 downregulated genes. By investigating potential key regulatory factors, comprehensive analysis indicated that changes in codon usage preference, amino acid metabolism, carbon metabolism, protein export processes, TGase activation, and spore production pathways collectively contributed to the enhancement of TGase activity. Subsequently, the in vitro activation efficiency of TGase was further improved using co-cultivation techniques with neutral proteases secreted by Bacillus amyloliquefaciens CICC10888, and a TGase activity of 16.91 U/mL was achieved, accounting for a 22.71% increase. This study provides a comprehensive understanding of the mechanisms underlying high-yield TGase production and valuable insights and data references for future research. |
| format | Article |
| id | doaj-art-a1a7694ca1f94558bd1726c919b17b8f |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-a1a7694ca1f94558bd1726c919b17b8f2025-02-05T07:32:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15256731525673Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategiesHuanan Chang0Ziyu Zheng1Hao Li2Yanqiu Xu3Gengyao Zhen4Yao Zhang5Xidong Ren6Xidong Ren7Xinli Liu8Xinli Liu9Deqiang Zhu10Deqiang Zhu11Shandong Provincial Key Laboratory of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Microbial Engineering, School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaTransglutaminase (TGase) has been widely applied in the food industry. However, achieving high-yield TGase production remains a challenge, limiting its broader industrial application. In this study, a high-yield strain with stable genetic traits was obtained through UV-ARTP combined mutagenesis, achieving a maximum TGase activity of 13.77 U/mL, representing a 92.43% increase. Using this strain as a forward mutation gene pool, comparative genomic research identified 95 mutated genes, which were mostly due to base substitutions that led to changes in codon usage preference. Transcriptomic analysis revealed significant expression changes in 470 genes, with 232 upregulated and 238 downregulated genes. By investigating potential key regulatory factors, comprehensive analysis indicated that changes in codon usage preference, amino acid metabolism, carbon metabolism, protein export processes, TGase activation, and spore production pathways collectively contributed to the enhancement of TGase activity. Subsequently, the in vitro activation efficiency of TGase was further improved using co-cultivation techniques with neutral proteases secreted by Bacillus amyloliquefaciens CICC10888, and a TGase activity of 16.91 U/mL was achieved, accounting for a 22.71% increase. This study provides a comprehensive understanding of the mechanisms underlying high-yield TGase production and valuable insights and data references for future research.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1525673/fulltransglutaminasecomplex mutationsgenomicstranscriptomicsco-culture fermentation |
| spellingShingle | Huanan Chang Ziyu Zheng Hao Li Yanqiu Xu Gengyao Zhen Yao Zhang Xidong Ren Xidong Ren Xinli Liu Xinli Liu Deqiang Zhu Deqiang Zhu Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategies Frontiers in Microbiology transglutaminase complex mutations genomics transcriptomics co-culture fermentation |
| title | Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategies |
| title_full | Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategies |
| title_fullStr | Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategies |
| title_full_unstemmed | Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategies |
| title_short | Multi-omics investigation of high-transglutaminase production mechanisms in Streptomyces mobaraensis and co-culture-enhanced fermentation strategies |
| title_sort | multi omics investigation of high transglutaminase production mechanisms in streptomyces mobaraensis and co culture enhanced fermentation strategies |
| topic | transglutaminase complex mutations genomics transcriptomics co-culture fermentation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1525673/full |
| work_keys_str_mv | AT huananchang multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT ziyuzheng multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT haoli multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT yanqiuxu multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT gengyaozhen multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT yaozhang multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT xidongren multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT xidongren multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT xinliliu multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT xinliliu multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT deqiangzhu multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies AT deqiangzhu multiomicsinvestigationofhightransglutaminaseproductionmechanismsinstreptomycesmobaraensisandcocultureenhancedfermentationstrategies |