A Simple, Rapid, and Cost-Effective Method for Assessing Carbohydrate Partitioning in Microalgae and Arabidopsis thaliana
Carbohydrates serve crucial functions in most living cells, encompassing structural and metabolic roles. Within the realms of plant and algal biology, carbohydrate biosynthesis and partitioning play pivotal roles in growth, development, stress physiology, and various practical applications. These ap...
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| Format: | Article |
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Bio-protocol LLC
2024-12-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5121&type=0 |
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| author | Araceli Bader Lara Rizza María De Marco Ana Lando Giselle Martínez-Noël Verónica Consolo Leonardo Curatti |
| author_facet | Araceli Bader Lara Rizza María De Marco Ana Lando Giselle Martínez-Noël Verónica Consolo Leonardo Curatti |
| author_sort | Araceli Bader |
| collection | DOAJ |
| description | Carbohydrates serve crucial functions in most living cells, encompassing structural and metabolic roles. Within the realms of plant and algal biology, carbohydrate biosynthesis and partitioning play pivotal roles in growth, development, stress physiology, and various practical applications. These applications span diverse fields, including the food and feed industry, bioenergetics (biofuels), and environmental management. However, existing methods for carbohydrate determination tend to be costly and time-intensive. In response to that, we propose a novel approach to assess carbohydrate partitioning from small samples. This method leverages the differential solubility of various fractions, including soluble sugars, starch, and structural polymers (such as cellulose). After fractionation, a straightforward spectrophotometric analysis allows for the quantification of sugars. |
| format | Article |
| id | doaj-art-a19fc6c3863e462bb98e0bfc25586ff5 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-a19fc6c3863e462bb98e0bfc25586ff52024-12-26T05:17:29ZengBio-protocol LLCBio-Protocol2331-83252024-12-01142310.21769/BioProtoc.5121A Simple, Rapid, and Cost-Effective Method for Assessing Carbohydrate Partitioning in Microalgae and Arabidopsis thalianaAraceli Bader0Lara Rizza1María De Marco2Ana Lando3Giselle Martínez-Noël4Verónica Consolo5Leonardo Curatti6Instituto de Investigaciones en Biodiversidad y Biotecnología and Fundación para Investigaciones Biológicas Aplicadas, Mar del Plata, Buenos Aires, ArgentinaInstituto de Investigaciones en Biodiversidad y Biotecnología and Fundación para Investigaciones Biológicas Aplicadas, Mar del Plata, Buenos Aires, ArgentinaUniversidad Federal de Paraná - Centro Politécnico. Departamento de Bioquímica y Biología Molecular, Curitiba, Paraná, BrasilInstituto de Investigaciones en Biodiversidad y Biotecnología and Fundación para Investigaciones Biológicas Aplicadas, Mar del Plata, Buenos Aires, ArgentinaInstituto de Investigaciones en Biodiversidad y Biotecnología and Fundación para Investigaciones Biológicas Aplicadas, Mar del Plata, Buenos Aires, ArgentinaInstituto de Investigaciones en Biodiversidad y Biotecnología and Fundación para Investigaciones Biológicas Aplicadas, Mar del Plata, Buenos Aires, ArgentinaInstituto de Investigaciones en Biodiversidad y Biotecnología and Fundación para Investigaciones Biológicas Aplicadas, Mar del Plata, Buenos Aires, ArgentinaInstituto de Investigaciones en Biodiversidad y Biotecnología and Fundación para Investigaciones Biológicas Aplicadas, Mar del Plata, Buenos Aires, ArgentinaCarbohydrates serve crucial functions in most living cells, encompassing structural and metabolic roles. Within the realms of plant and algal biology, carbohydrate biosynthesis and partitioning play pivotal roles in growth, development, stress physiology, and various practical applications. These applications span diverse fields, including the food and feed industry, bioenergetics (biofuels), and environmental management. However, existing methods for carbohydrate determination tend to be costly and time-intensive. In response to that, we propose a novel approach to assess carbohydrate partitioning from small samples. This method leverages the differential solubility of various fractions, including soluble sugars, starch, and structural polymers (such as cellulose). After fractionation, a straightforward spectrophotometric analysis allows for the quantification of sugars.https://bio-protocol.org/en/bpdetail?id=5121&type=0 |
| spellingShingle | Araceli Bader Lara Rizza María De Marco Ana Lando Giselle Martínez-Noël Verónica Consolo Leonardo Curatti A Simple, Rapid, and Cost-Effective Method for Assessing Carbohydrate Partitioning in Microalgae and Arabidopsis thaliana Bio-Protocol |
| title | A Simple, Rapid, and Cost-Effective Method for Assessing Carbohydrate Partitioning in Microalgae and Arabidopsis thaliana |
| title_full | A Simple, Rapid, and Cost-Effective Method for Assessing Carbohydrate Partitioning in Microalgae and Arabidopsis thaliana |
| title_fullStr | A Simple, Rapid, and Cost-Effective Method for Assessing Carbohydrate Partitioning in Microalgae and Arabidopsis thaliana |
| title_full_unstemmed | A Simple, Rapid, and Cost-Effective Method for Assessing Carbohydrate Partitioning in Microalgae and Arabidopsis thaliana |
| title_short | A Simple, Rapid, and Cost-Effective Method for Assessing Carbohydrate Partitioning in Microalgae and Arabidopsis thaliana |
| title_sort | simple rapid and cost effective method for assessing carbohydrate partitioning in microalgae and arabidopsis thaliana |
| url | https://bio-protocol.org/en/bpdetail?id=5121&type=0 |
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