Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent
An impressively simple and precise spectrofluorimetric procedure was established and validated for ponatinib (PTB) quantitation in biological fluids such as human plasma and human urine. This method depends on examining the fluorescence characteristics of PTB in a micellar system of Cremophor RH 40...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/210503 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561724144746496 |
|---|---|
| author | Hany W. Darwish Ahmed H. Bakheit Ali Saber Abdelhameed Amer S. AlKhairallah |
| author_facet | Hany W. Darwish Ahmed H. Bakheit Ali Saber Abdelhameed Amer S. AlKhairallah |
| author_sort | Hany W. Darwish |
| collection | DOAJ |
| description | An impressively simple and precise spectrofluorimetric procedure was established and validated for ponatinib (PTB) quantitation in biological fluids such as human plasma and human urine. This method depends on examining the fluorescence characteristics of PTB in a micellar system of Cremophor RH 40 (Cr RH 40). Cr RH 40 enhanced the intrinsic fluorescence of PTB distinctly in aqueous water. The fluorescence spectra of PTB was recorded at 457 nm following its excitation at 305 nm. Maximum fluorescence intensity was attained by addition of 0.7 mL of Cr RH 40 and one mL of phosphate buffer to PTB aliquots and then dilution with distilled water. There is a linear relationship between the fluorescence intensity of PTB and its concentration over the range 5–120 ngmL−1, with limit of detection and limit of quantification equal to 0.905 ngmL−1 and 2.742 ngmL−1, respectively. The accuracy and the precisions of the proposed method were checked and gave adequate results. The adopted method was applied with a great success for PTB quantitation in different biological matrices (spiked human plasma and urine) giving high recovery values. |
| format | Article |
| id | doaj-art-a1908ecaaa2b4ea5b5ae007a3b5532a7 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-a1908ecaaa2b4ea5b5ae007a3b5532a72025-02-03T01:24:22ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792015-01-01201510.1155/2015/210503210503Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing AgentHany W. Darwish0Ahmed H. Bakheit1Ali Saber Abdelhameed2Amer S. AlKhairallah3Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaQuality Management Department, National Guard Health Affairs, King Abdulaziz Medical City, P.O. Box 22490, Riyadh 11426, Saudi ArabiaAn impressively simple and precise spectrofluorimetric procedure was established and validated for ponatinib (PTB) quantitation in biological fluids such as human plasma and human urine. This method depends on examining the fluorescence characteristics of PTB in a micellar system of Cremophor RH 40 (Cr RH 40). Cr RH 40 enhanced the intrinsic fluorescence of PTB distinctly in aqueous water. The fluorescence spectra of PTB was recorded at 457 nm following its excitation at 305 nm. Maximum fluorescence intensity was attained by addition of 0.7 mL of Cr RH 40 and one mL of phosphate buffer to PTB aliquots and then dilution with distilled water. There is a linear relationship between the fluorescence intensity of PTB and its concentration over the range 5–120 ngmL−1, with limit of detection and limit of quantification equal to 0.905 ngmL−1 and 2.742 ngmL−1, respectively. The accuracy and the precisions of the proposed method were checked and gave adequate results. The adopted method was applied with a great success for PTB quantitation in different biological matrices (spiked human plasma and urine) giving high recovery values.http://dx.doi.org/10.1155/2015/210503 |
| spellingShingle | Hany W. Darwish Ahmed H. Bakheit Ali Saber Abdelhameed Amer S. AlKhairallah Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent International Journal of Analytical Chemistry |
| title | Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent |
| title_full | Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent |
| title_fullStr | Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent |
| title_full_unstemmed | Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent |
| title_short | Micellar Enhanced Spectrofluorimetric Method for the Determination of Ponatinib in Human Plasma and Urine via Cremophor RH 40 as Sensing Agent |
| title_sort | micellar enhanced spectrofluorimetric method for the determination of ponatinib in human plasma and urine via cremophor rh 40 as sensing agent |
| url | http://dx.doi.org/10.1155/2015/210503 |
| work_keys_str_mv | AT hanywdarwish micellarenhancedspectrofluorimetricmethodforthedeterminationofponatinibinhumanplasmaandurineviacremophorrh40assensingagent AT ahmedhbakheit micellarenhancedspectrofluorimetricmethodforthedeterminationofponatinibinhumanplasmaandurineviacremophorrh40assensingagent AT alisaberabdelhameed micellarenhancedspectrofluorimetricmethodforthedeterminationofponatinibinhumanplasmaandurineviacremophorrh40assensingagent AT amersalkhairallah micellarenhancedspectrofluorimetricmethodforthedeterminationofponatinibinhumanplasmaandurineviacremophorrh40assensingagent |