Comparative Physiological and Transcriptome Analyses Reveal the Responses to Ocean Acidification Challenge of <i>Mactra veneriformis</i> with Different Shell Colors
<i>Mactra veneriformis</i> is highly susceptible to ocean acidification (OA) due to its low shell hardness during its rapid growth period. In this study, oxygen consumption rate, ammonia excretion rate, and transcriptome sequencing of mantle tissue analyses were conducted in white and pu...
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2024-11-01
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| author | Limei Chen Gengyin Huang Shuang Liang Jian Liang Hefeng Wang Huiru Liu Xiaoyu Wang Li Li Yongjun Guo Wenguang Liu |
| author_facet | Limei Chen Gengyin Huang Shuang Liang Jian Liang Hefeng Wang Huiru Liu Xiaoyu Wang Li Li Yongjun Guo Wenguang Liu |
| author_sort | Limei Chen |
| collection | DOAJ |
| description | <i>Mactra veneriformis</i> is highly susceptible to ocean acidification (OA) due to its low shell hardness during its rapid growth period. In this study, oxygen consumption rate, ammonia excretion rate, and transcriptome sequencing of mantle tissue analyses were conducted in white and purple shell-color populations of <i>M. veneriformis</i> under OA stress (pH = 7.6). The findings indicated a significant rise in oxygen consumption rates and ammonia excretion rates following acidification in both the two shell colors, while the clams with purple color showed comparatively lower basal metabolic levels. Transcriptomic analyses demonstrated the expression of key genes related to fatty acid synthesis were significantly inhibited, whereas genes involved in calcification, osmoregulation, and immune response were upregulated under OA exposure in the two shell-color groups. However, some genes such as CA and HSP showed a population-specific response between the two shell-color populations. KEGG enrichment analysis revealed that the MAPK signaling pathway and protein processing in the endoplasmic reticulum were significantly enriched in the two acidification groups. This study provides valuable insights into the response of <i>M. veneriformis</i> to OA stress and also helps to predict the future breeding of valuable strains of <i>M. veneriformis</i>. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-a17bf7f0f8514b9499f3c29ce4c8bdc32024-12-27T14:25:54ZengMDPI AGFishes2410-38882024-11-0191248610.3390/fishes9120486Comparative Physiological and Transcriptome Analyses Reveal the Responses to Ocean Acidification Challenge of <i>Mactra veneriformis</i> with Different Shell ColorsLimei Chen0Gengyin Huang1Shuang Liang2Jian Liang3Hefeng Wang4Huiru Liu5Xiaoyu Wang6Li Li7Yongjun Guo8Wenguang Liu9Tianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, ChinaTianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, ChinaTianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, ChinaTianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, ChinaTianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, ChinaTianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, ChinaTianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, ChinaMarine Science Research Institute of Shandong Province (National Oceanographic Center, Qingdao), Qingdao 266104, ChinaTianjin Key Laboratory of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300392, ChinaGuangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China<i>Mactra veneriformis</i> is highly susceptible to ocean acidification (OA) due to its low shell hardness during its rapid growth period. In this study, oxygen consumption rate, ammonia excretion rate, and transcriptome sequencing of mantle tissue analyses were conducted in white and purple shell-color populations of <i>M. veneriformis</i> under OA stress (pH = 7.6). The findings indicated a significant rise in oxygen consumption rates and ammonia excretion rates following acidification in both the two shell colors, while the clams with purple color showed comparatively lower basal metabolic levels. Transcriptomic analyses demonstrated the expression of key genes related to fatty acid synthesis were significantly inhibited, whereas genes involved in calcification, osmoregulation, and immune response were upregulated under OA exposure in the two shell-color groups. However, some genes such as CA and HSP showed a population-specific response between the two shell-color populations. KEGG enrichment analysis revealed that the MAPK signaling pathway and protein processing in the endoplasmic reticulum were significantly enriched in the two acidification groups. This study provides valuable insights into the response of <i>M. veneriformis</i> to OA stress and also helps to predict the future breeding of valuable strains of <i>M. veneriformis</i>.https://www.mdpi.com/2410-3888/9/12/486<i>Mactra veneriformis</i>ocean acidificationoxygen consumption rateammonia excretion ratetranscriptome analysis |
| spellingShingle | Limei Chen Gengyin Huang Shuang Liang Jian Liang Hefeng Wang Huiru Liu Xiaoyu Wang Li Li Yongjun Guo Wenguang Liu Comparative Physiological and Transcriptome Analyses Reveal the Responses to Ocean Acidification Challenge of <i>Mactra veneriformis</i> with Different Shell Colors Fishes <i>Mactra veneriformis</i> ocean acidification oxygen consumption rate ammonia excretion rate transcriptome analysis |
| title | Comparative Physiological and Transcriptome Analyses Reveal the Responses to Ocean Acidification Challenge of <i>Mactra veneriformis</i> with Different Shell Colors |
| title_full | Comparative Physiological and Transcriptome Analyses Reveal the Responses to Ocean Acidification Challenge of <i>Mactra veneriformis</i> with Different Shell Colors |
| title_fullStr | Comparative Physiological and Transcriptome Analyses Reveal the Responses to Ocean Acidification Challenge of <i>Mactra veneriformis</i> with Different Shell Colors |
| title_full_unstemmed | Comparative Physiological and Transcriptome Analyses Reveal the Responses to Ocean Acidification Challenge of <i>Mactra veneriformis</i> with Different Shell Colors |
| title_short | Comparative Physiological and Transcriptome Analyses Reveal the Responses to Ocean Acidification Challenge of <i>Mactra veneriformis</i> with Different Shell Colors |
| title_sort | comparative physiological and transcriptome analyses reveal the responses to ocean acidification challenge of i mactra veneriformis i with different shell colors |
| topic | <i>Mactra veneriformis</i> ocean acidification oxygen consumption rate ammonia excretion rate transcriptome analysis |
| url | https://www.mdpi.com/2410-3888/9/12/486 |
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