Histamine as a marker for hydroxyl radicals
During inflammation an influx of neutrophils and release of mediators from mast cells (such as histamine) take place. The stimulated neutrophils can produce reactive oxygen species (ROS). One of these ROS is the highly reactive hydroxyl radical (OH.). It would be interesting to be able to quantify t...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000548 |
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| _version_ | 1849398417361469440 |
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| author | T.-L. Ching R. M. van der Hee N. M. Bhoelan J. Blauw W. M. P. B. Menge J. de Jong A. Bast |
| author_facet | T.-L. Ching R. M. van der Hee N. M. Bhoelan J. Blauw W. M. P. B. Menge J. de Jong A. Bast |
| author_sort | T.-L. Ching |
| collection | DOAJ |
| description | During inflammation an influx of neutrophils and release of mediators from mast cells (such as histamine) take place. The stimulated neutrophils can produce reactive oxygen species (ROS). One of these ROS is the highly reactive hydroxyl radical (OH.). It would be interesting to be able to quantify the extent of ROS formation. We investigated if histamine which is present at the inflammation site can serve as an endogenous marker for the formation of OH.. We found that histamine after incubation with OH. gave two distinct products in our HPLC system. One of the products gave the same characteristics as the synthesized 2-imidazolone derivative of histamine. This suggests that this derivative will be formed when histamine is incubated with OH.. |
| format | Article |
| id | doaj-art-a178d237004048beb0a5caa4cf35b692 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-a178d237004048beb0a5caa4cf35b6922025-08-20T03:38:38ZengWileyMediators of Inflammation0962-93511466-18611995-01-014533934310.1155/S0962935195000548Histamine as a marker for hydroxyl radicalsT.-L. Ching0R. M. van der Hee1N. M. Bhoelan2J. Blauw3W. M. P. B. Menge4J. de Jong5A. Bast6Leiden/Amsterdam Center for Drug Research, Division of Molecular Pharmacology, Department of Pharmacochemistry, Vrije Universiteit Amsterdam, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsLeiden/Amsterdam Center for Drug Research, Division of Molecular Pharmacology, Department of Pharmacochemistry, Vrije Universiteit Amsterdam, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsLeiden/Amsterdam Center for Drug Research, Division of Molecular Pharmacology, Department of Pharmacochemistry, Vrije Universiteit Amsterdam, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsLeiden/Amsterdam Center for Drug Research, Division of Medicinal Chemistry, Department of Pharmacochemistry, Vrije Universiteit Amsterdam, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsLeiden/Amsterdam Center for Drug Research, Division of Medicinal Chemistry, Department of Pharmacochemistry, Vrije Universiteit Amsterdam, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsLeiden/Amsterdam Center for Drug Research, Division of Molecular Pharmacology, Department of Pharmacochemistry, Vrije Universiteit Amsterdam, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsLeiden/Amsterdam Center for Drug Research, Division of Molecular Pharmacology, Department of Pharmacochemistry, Vrije Universiteit Amsterdam, De Boelelaan 1083, Amsterdam 1081 HV, The NetherlandsDuring inflammation an influx of neutrophils and release of mediators from mast cells (such as histamine) take place. The stimulated neutrophils can produce reactive oxygen species (ROS). One of these ROS is the highly reactive hydroxyl radical (OH.). It would be interesting to be able to quantify the extent of ROS formation. We investigated if histamine which is present at the inflammation site can serve as an endogenous marker for the formation of OH.. We found that histamine after incubation with OH. gave two distinct products in our HPLC system. One of the products gave the same characteristics as the synthesized 2-imidazolone derivative of histamine. This suggests that this derivative will be formed when histamine is incubated with OH..http://dx.doi.org/10.1155/S0962935195000548 |
| spellingShingle | T.-L. Ching R. M. van der Hee N. M. Bhoelan J. Blauw W. M. P. B. Menge J. de Jong A. Bast Histamine as a marker for hydroxyl radicals Mediators of Inflammation |
| title | Histamine as a marker for hydroxyl radicals |
| title_full | Histamine as a marker for hydroxyl radicals |
| title_fullStr | Histamine as a marker for hydroxyl radicals |
| title_full_unstemmed | Histamine as a marker for hydroxyl radicals |
| title_short | Histamine as a marker for hydroxyl radicals |
| title_sort | histamine as a marker for hydroxyl radicals |
| url | http://dx.doi.org/10.1155/S0962935195000548 |
| work_keys_str_mv | AT tlching histamineasamarkerforhydroxylradicals AT rmvanderhee histamineasamarkerforhydroxylradicals AT nmbhoelan histamineasamarkerforhydroxylradicals AT jblauw histamineasamarkerforhydroxylradicals AT wmpbmenge histamineasamarkerforhydroxylradicals AT jdejong histamineasamarkerforhydroxylradicals AT abast histamineasamarkerforhydroxylradicals |