In Vitro Anti-Glioblastoma Activity of <i>Echinocereus engelmannii-</i> and <i>Echinocereus pectinatus</i>-Associated Bacterial Endophyte Extracts
Glioblastoma is the most common and aggressive brain tumor in adults. However, due to the limitations of conventional treatments, as well as their side effects, there is a need to develop more effective and less harmful therapy strategies. There is evidence that plants endemic to northern Mexico pos...
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2025-03-01
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| author | Ana L. Delgado-Miranda Ricardo Gomez-Flores Nancy E. Rodríguez-Garza Orquídea Pérez-González Patricia Tamez-Guerra Diana Caballero-Hernández Diana L. Clark-Pérez Ramiro Quintanilla-Licea Andrés García César I. Romo-Sáenz |
| author_facet | Ana L. Delgado-Miranda Ricardo Gomez-Flores Nancy E. Rodríguez-Garza Orquídea Pérez-González Patricia Tamez-Guerra Diana Caballero-Hernández Diana L. Clark-Pérez Ramiro Quintanilla-Licea Andrés García César I. Romo-Sáenz |
| author_sort | Ana L. Delgado-Miranda |
| collection | DOAJ |
| description | Glioblastoma is the most common and aggressive brain tumor in adults. However, due to the limitations of conventional treatments, as well as their side effects, there is a need to develop more effective and less harmful therapy strategies. There is evidence that plants endemic to northern Mexico possess biological activities that positively impact human health, particularly against cancer. <i>Echinocereus engelmannii</i> and <i>Echinocereus pectinatus</i> are cacti from the north of Mexico that produce bioactive compounds with antitumor activity. We obtained methanol extracts from previously isolated and fermented microorganisms associated with these cacti. Cell lines of extracts with cytotoxicity against glioblastoma cells U87, neuroblastoma cells SH-S5Y5, and Schwann neuronal cells (healthy control) were evaluated, using a colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazole bromide (MTT) reduction technique. The selective cytotoxicity extracts were analyzed using liquid chromatography tandem mass spectrometry (LC/MS<sup>2</sup>). We isolated 19 endophytic and soil-associated microorganisms from both cacti. Two of them were selected for their high percentages of tumor growth inhibition. The microorganism ES4 possessed the best activity with an IC<sub>50</sub> of 17.31 ± 1.70 µg/mL and a selectivity index of 3.11. We identified the bacterium <i>Stenotrophomonas maltophilia</i> by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) from the most active microorganisms against tumor growth. LC/MS<sup>2</sup> characterized the HS4 extract, and the most abundant group (50.0%) identified included carboxylic acids and derivatives, particularly bisgerayafolin A, Cyclo (Pro-Leu), maculosin, and tryptophan. In conclusion <i>S. maltophilia</i> extract inhibit the growth of glioma cells, showing greater sensitivity in the U87 cell line. |
| format | Article |
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| institution | DOAJ |
| issn | 2075-1729 |
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| publishDate | 2025-03-01 |
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| spelling | doaj-art-a167e82399174486b48a79474fc3cf4e2025-08-20T03:13:50ZengMDPI AGLife2075-17292025-03-0115451910.3390/life15040519In Vitro Anti-Glioblastoma Activity of <i>Echinocereus engelmannii-</i> and <i>Echinocereus pectinatus</i>-Associated Bacterial Endophyte ExtractsAna L. Delgado-Miranda0Ricardo Gomez-Flores1Nancy E. Rodríguez-Garza2Orquídea Pérez-González3Patricia Tamez-Guerra4Diana Caballero-Hernández5Diana L. Clark-Pérez6Ramiro Quintanilla-Licea7Andrés García8César I. Romo-Sáenz9Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Nuevo León, MexicoDepartamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Nuevo León, MexicoDepartamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Nuevo León, MexicoDepartamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Nuevo León, MexicoDepartamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Nuevo León, MexicoDepartamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Nuevo León, MexicoDepartamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Nuevo León, MexicoDepartamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Nuevo León, MexicoLaboratorio de Biotecnología Ambiental, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, MexicoDepartamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Nuevo León, MexicoGlioblastoma is the most common and aggressive brain tumor in adults. However, due to the limitations of conventional treatments, as well as their side effects, there is a need to develop more effective and less harmful therapy strategies. There is evidence that plants endemic to northern Mexico possess biological activities that positively impact human health, particularly against cancer. <i>Echinocereus engelmannii</i> and <i>Echinocereus pectinatus</i> are cacti from the north of Mexico that produce bioactive compounds with antitumor activity. We obtained methanol extracts from previously isolated and fermented microorganisms associated with these cacti. Cell lines of extracts with cytotoxicity against glioblastoma cells U87, neuroblastoma cells SH-S5Y5, and Schwann neuronal cells (healthy control) were evaluated, using a colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazole bromide (MTT) reduction technique. The selective cytotoxicity extracts were analyzed using liquid chromatography tandem mass spectrometry (LC/MS<sup>2</sup>). We isolated 19 endophytic and soil-associated microorganisms from both cacti. Two of them were selected for their high percentages of tumor growth inhibition. The microorganism ES4 possessed the best activity with an IC<sub>50</sub> of 17.31 ± 1.70 µg/mL and a selectivity index of 3.11. We identified the bacterium <i>Stenotrophomonas maltophilia</i> by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) from the most active microorganisms against tumor growth. LC/MS<sup>2</sup> characterized the HS4 extract, and the most abundant group (50.0%) identified included carboxylic acids and derivatives, particularly bisgerayafolin A, Cyclo (Pro-Leu), maculosin, and tryptophan. In conclusion <i>S. maltophilia</i> extract inhibit the growth of glioma cells, showing greater sensitivity in the U87 cell line.https://www.mdpi.com/2075-1729/15/4/519<i>Stenotrophomonas maltophilia</i><i>Echinocereus engelmannii</i><i>Echinocereus pectinatus</i>antitumor activitycytotoxic activity |
| spellingShingle | Ana L. Delgado-Miranda Ricardo Gomez-Flores Nancy E. Rodríguez-Garza Orquídea Pérez-González Patricia Tamez-Guerra Diana Caballero-Hernández Diana L. Clark-Pérez Ramiro Quintanilla-Licea Andrés García César I. Romo-Sáenz In Vitro Anti-Glioblastoma Activity of <i>Echinocereus engelmannii-</i> and <i>Echinocereus pectinatus</i>-Associated Bacterial Endophyte Extracts Life <i>Stenotrophomonas maltophilia</i> <i>Echinocereus engelmannii</i> <i>Echinocereus pectinatus</i> antitumor activity cytotoxic activity |
| title | In Vitro Anti-Glioblastoma Activity of <i>Echinocereus engelmannii-</i> and <i>Echinocereus pectinatus</i>-Associated Bacterial Endophyte Extracts |
| title_full | In Vitro Anti-Glioblastoma Activity of <i>Echinocereus engelmannii-</i> and <i>Echinocereus pectinatus</i>-Associated Bacterial Endophyte Extracts |
| title_fullStr | In Vitro Anti-Glioblastoma Activity of <i>Echinocereus engelmannii-</i> and <i>Echinocereus pectinatus</i>-Associated Bacterial Endophyte Extracts |
| title_full_unstemmed | In Vitro Anti-Glioblastoma Activity of <i>Echinocereus engelmannii-</i> and <i>Echinocereus pectinatus</i>-Associated Bacterial Endophyte Extracts |
| title_short | In Vitro Anti-Glioblastoma Activity of <i>Echinocereus engelmannii-</i> and <i>Echinocereus pectinatus</i>-Associated Bacterial Endophyte Extracts |
| title_sort | in vitro anti glioblastoma activity of i echinocereus engelmannii i and i echinocereus pectinatus i associated bacterial endophyte extracts |
| topic | <i>Stenotrophomonas maltophilia</i> <i>Echinocereus engelmannii</i> <i>Echinocereus pectinatus</i> antitumor activity cytotoxic activity |
| url | https://www.mdpi.com/2075-1729/15/4/519 |
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