Monocytes Do Not Transdifferentiate into Proper Osteoblasts
Recent publications suggested that monocytes might be an attractive cell type to transdifferentiate into various cellular phenotypes. Aim was, therefore, to evaluate the potential of blood monocytes to transdifferentiate into osteoblasts. Monocytes isolated from peripheral blood were subjected to tw...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/384936 |
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| _version_ | 1841524477113925632 |
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| author | Andreas Schmitt Sabrina Ehnert Lilianna Schyschka Peter Buschner Andreas Kühnl Stefan Döbele Sebastian Siebenlist Martin Lucke Ulrich Stöckle Andreas K. Nussler |
| author_facet | Andreas Schmitt Sabrina Ehnert Lilianna Schyschka Peter Buschner Andreas Kühnl Stefan Döbele Sebastian Siebenlist Martin Lucke Ulrich Stöckle Andreas K. Nussler |
| author_sort | Andreas Schmitt |
| collection | DOAJ |
| description | Recent publications suggested that monocytes might be an attractive cell type to transdifferentiate into various cellular phenotypes. Aim was, therefore, to evaluate the potential of blood monocytes to transdifferentiate into osteoblasts. Monocytes isolated from peripheral blood were subjected to two previously published treatments to obtain unique, multipotent cell fractions, named programmable cells of monocytic origin (PCMOs) and monocyte-derived mesenchymal progenitor cells (MOMPs). Subsequently, MOMPs and PCMOs were treated with osteogenic differentiation medium (including either vitamin D or dexamethasone) for 14 days. Regarding a variety of surface markers, no differences between MOMPs, PCMOs, and primary monocytes could be detected. The treatment with osteogenic medium neither resulted in loss of hematopoietic markers nor in adoption of mesenchymal phenotype in all cell types. No significant effect was observed regarding the expression of osteogenic transcription factors, bone-related genes, or production of mineralized matrix. Osteogenic medium resulted in activation of monocytes and appearance of osteoclasts. In conclusion, none of the investigated monocyte cell types showed any transdifferentiation characteristics under the tested circumstances. Based on our data, we rather see an activation and maturation of monocytes towards macrophages and osteoclasts. |
| format | Article |
| id | doaj-art-a160bd4f663745878672014493751582 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-a160bd4f6637458786720144937515822025-02-03T05:53:07ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/384936384936Monocytes Do Not Transdifferentiate into Proper OsteoblastsAndreas Schmitt0Sabrina Ehnert1Lilianna Schyschka2Peter Buschner3Andreas Kühnl4Stefan Döbele5Sebastian Siebenlist6Martin Lucke7Ulrich Stöckle8Andreas K. Nussler9Department of Traumatology, MRI, Technical University Munich, 81675 Munich, GermanyEberhard-Karls University, BG Trauma Clinic, 72076 Tübingen, GermanyDepartment of Traumatology, MRI, Technical University Munich, 81675 Munich, GermanyDepartment of Traumatology, MRI, Technical University Munich, 81675 Munich, GermanyDepartment of Vascular Surgery, MRI, Technical University Munich, 81675 Munich, GermanyDepartment of Traumatology, MRI, Technical University Munich, 81675 Munich, GermanyDepartment of Traumatology, MRI, Technical University Munich, 81675 Munich, GermanyDepartment of Traumatology, MRI, Technical University Munich, 81675 Munich, GermanyEberhard-Karls University, BG Trauma Clinic, 72076 Tübingen, GermanyEberhard-Karls University, BG Trauma Clinic, 72076 Tübingen, GermanyRecent publications suggested that monocytes might be an attractive cell type to transdifferentiate into various cellular phenotypes. Aim was, therefore, to evaluate the potential of blood monocytes to transdifferentiate into osteoblasts. Monocytes isolated from peripheral blood were subjected to two previously published treatments to obtain unique, multipotent cell fractions, named programmable cells of monocytic origin (PCMOs) and monocyte-derived mesenchymal progenitor cells (MOMPs). Subsequently, MOMPs and PCMOs were treated with osteogenic differentiation medium (including either vitamin D or dexamethasone) for 14 days. Regarding a variety of surface markers, no differences between MOMPs, PCMOs, and primary monocytes could be detected. The treatment with osteogenic medium neither resulted in loss of hematopoietic markers nor in adoption of mesenchymal phenotype in all cell types. No significant effect was observed regarding the expression of osteogenic transcription factors, bone-related genes, or production of mineralized matrix. Osteogenic medium resulted in activation of monocytes and appearance of osteoclasts. In conclusion, none of the investigated monocyte cell types showed any transdifferentiation characteristics under the tested circumstances. Based on our data, we rather see an activation and maturation of monocytes towards macrophages and osteoclasts.http://dx.doi.org/10.1100/2012/384936 |
| spellingShingle | Andreas Schmitt Sabrina Ehnert Lilianna Schyschka Peter Buschner Andreas Kühnl Stefan Döbele Sebastian Siebenlist Martin Lucke Ulrich Stöckle Andreas K. Nussler Monocytes Do Not Transdifferentiate into Proper Osteoblasts The Scientific World Journal |
| title | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
| title_full | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
| title_fullStr | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
| title_full_unstemmed | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
| title_short | Monocytes Do Not Transdifferentiate into Proper Osteoblasts |
| title_sort | monocytes do not transdifferentiate into proper osteoblasts |
| url | http://dx.doi.org/10.1100/2012/384936 |
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