Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa
Introduction: Salmonella is well recognized as an aetiological agent of gastrointestinal and diarrhoeal disease. Salmonella enterica serotype Enteritidis (Salmonella Enteritidis) is one of the commonest serotypes associated with foodborne illness. In South Africa, we compared Salmonella Enteritidis...
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The Journal of Infection in Developing Countries
2014-12-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/5393 |
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| author | Anthony Marius Smith Husna Ismail Maryke M Henton Karen H Keddy GERMS-SA Surveillance Network |
| author_facet | Anthony Marius Smith Husna Ismail Maryke M Henton Karen H Keddy GERMS-SA Surveillance Network |
| author_sort | Anthony Marius Smith |
| collection | DOAJ |
| description | Introduction: Salmonella is well recognized as an aetiological agent of gastrointestinal and diarrhoeal disease. Salmonella enterica serotype Enteritidis (Salmonella Enteritidis) is one of the commonest serotypes associated with foodborne illness. In South Africa, we compared Salmonella Enteritidis strains isolated from humans with gastroenteritis and strains isolated from captive wild animals, between June 2011 and July 2012.
Methodology: Bacteria were phenotypically characterized using standard microbiological techniques. Genotypic relatedness of isolates was investigated by pulsed-field gel electrophoresis (PFGE) analysis.
Results: a diversity of 27 PFGE patterns amongst 196 human non-invasive isolates was shown; two PFGE patterns predominated and accounted for 74% of all human isolates. Human isolates showed a 12% prevalence rate for nalidixic acid resistance. Animal isolates from 5 different sources were investigated. With the exception of an isolate from a ground hornbill, all animal isolates (jaguar, crocodile, lion and poultry) showed PFGE pattern matches to a human isolate. Animal isolates showed susceptibility to all antimicrobial agents tested, with the exception of nalidixic acid resistance in isolates from the lion and poultry source.
Conclusions: Our data showed similarities between Salmonella Enteritidis strains isolated from humans and captive wild animals, suggesting a probable common source for strains from humans and animals.
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| format | Article |
| id | doaj-art-a15f1cc798e7467590c1d137fe19c652 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-a15f1cc798e7467590c1d137fe19c6522025-08-20T02:57:45ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802014-12-0181210.3855/jidc.5393Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South AfricaAnthony Marius Smith0Husna Ismail1Maryke M Henton2Karen H Keddy3GERMS-SA Surveillance NetworkCentre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service, Johannesburg, South AfricaCentre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service, Johannesburg, South AfricaIdexx Laboratories, Johannesburg, South AfricaCentre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service, Johannesburg, South AfricaIntroduction: Salmonella is well recognized as an aetiological agent of gastrointestinal and diarrhoeal disease. Salmonella enterica serotype Enteritidis (Salmonella Enteritidis) is one of the commonest serotypes associated with foodborne illness. In South Africa, we compared Salmonella Enteritidis strains isolated from humans with gastroenteritis and strains isolated from captive wild animals, between June 2011 and July 2012. Methodology: Bacteria were phenotypically characterized using standard microbiological techniques. Genotypic relatedness of isolates was investigated by pulsed-field gel electrophoresis (PFGE) analysis. Results: a diversity of 27 PFGE patterns amongst 196 human non-invasive isolates was shown; two PFGE patterns predominated and accounted for 74% of all human isolates. Human isolates showed a 12% prevalence rate for nalidixic acid resistance. Animal isolates from 5 different sources were investigated. With the exception of an isolate from a ground hornbill, all animal isolates (jaguar, crocodile, lion and poultry) showed PFGE pattern matches to a human isolate. Animal isolates showed susceptibility to all antimicrobial agents tested, with the exception of nalidixic acid resistance in isolates from the lion and poultry source. Conclusions: Our data showed similarities between Salmonella Enteritidis strains isolated from humans and captive wild animals, suggesting a probable common source for strains from humans and animals. https://jidc.org/index.php/journal/article/view/5393Salmonella Enteritidiswild animalpulsed-field gel electrophoresisPFGEPulseNet |
| spellingShingle | Anthony Marius Smith Husna Ismail Maryke M Henton Karen H Keddy GERMS-SA Surveillance Network Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa Journal of Infection in Developing Countries Salmonella Enteritidis wild animal pulsed-field gel electrophoresis PFGE PulseNet |
| title | Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa |
| title_full | Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa |
| title_fullStr | Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa |
| title_full_unstemmed | Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa |
| title_short | Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa |
| title_sort | similarities between salmonella enteritidis isolated from humans and captive wild animals in south africa |
| topic | Salmonella Enteritidis wild animal pulsed-field gel electrophoresis PFGE PulseNet |
| url | https://jidc.org/index.php/journal/article/view/5393 |
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