Poly-sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birth
Abstract Preterm birth is a global health issue associated with neonatal death and morbidity. However, current methods of predicting preterm birth are insufficient to accurately screen for risk. This study aimed to assess the potential of site-specific N-glycosylation of cervicovaginal fluid (CVF) p...
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Nature Portfolio
2025-04-01
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| Online Access: | https://doi.org/10.1038/s41598-025-96682-4 |
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| author | Yoon-Young Go Gun Wook Park Young Min Hur Young-Ah You Gain Lee Rin Chae Soo-Min Kim Sunwha Park Young Ju Kim |
| author_facet | Yoon-Young Go Gun Wook Park Young Min Hur Young-Ah You Gain Lee Rin Chae Soo-Min Kim Sunwha Park Young Ju Kim |
| author_sort | Yoon-Young Go |
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| description | Abstract Preterm birth is a global health issue associated with neonatal death and morbidity. However, current methods of predicting preterm birth are insufficient to accurately screen for risk. This study aimed to assess the potential of site-specific N-glycosylation of cervicovaginal fluid (CVF) proteins as predictive biomarkers of preterm birth in a case-control study. Statistical analysis used Student’s t-tests, ROC curve and logistic regression adjusted age and BMI. Using N-glycoproteomic analysis of the CVF, we identified 862 N-glycoproteins in CVF samples form 20 pregnancies and 6595 N-linked glycopeptides used a false discovery rate of less than 1%. Of 173 upregulated glycan in preterm group, we found low levels of fucosylation and high levels of sialylation in preterm birth (p < 0.05). Then we found that three poly-sialylated glycans had a high predictive value (AUC = 0.802, p < 0.017), which were expressed in all samples. In addition, the glycan model with clinical markers performed better. The results indicate that poly-sialylated glycans in CVF have potential value as novel clinical markers for predicting preterm birth during pregnancy. This study suggests strategies for developing new predictive biomarkers using cervicovaginal glycans to detect preterm birth in advance. |
| format | Article |
| id | doaj-art-a151d8f71cd2483da3019b75330cfb48 |
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| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-a151d8f71cd2483da3019b75330cfb482025-08-20T01:53:11ZengNature PortfolioScientific Reports2045-23222025-04-011511910.1038/s41598-025-96682-4Poly-sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birthYoon-Young Go0Gun Wook Park1Young Min Hur2Young-Ah You3Gain Lee4Rin Chae5Soo-Min Kim6Sunwha Park7Young Ju Kim8Department of Obstetrics and Gynecology, College of Medicine and Ewha Medical Institute, Ewha Womans UniversityBioinformatics Lab, CellKey IncDepartment of Obstetrics and Gynecology, College of Medicine and Ewha Medical Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine and Ewha Medical Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine and Ewha Medical Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine and Ewha Medical Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine and Ewha Medical Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine and Ewha Medical Institute, Ewha Womans UniversityDepartment of Obstetrics and Gynecology, College of Medicine and Ewha Medical Institute, Ewha Womans UniversityAbstract Preterm birth is a global health issue associated with neonatal death and morbidity. However, current methods of predicting preterm birth are insufficient to accurately screen for risk. This study aimed to assess the potential of site-specific N-glycosylation of cervicovaginal fluid (CVF) proteins as predictive biomarkers of preterm birth in a case-control study. Statistical analysis used Student’s t-tests, ROC curve and logistic regression adjusted age and BMI. Using N-glycoproteomic analysis of the CVF, we identified 862 N-glycoproteins in CVF samples form 20 pregnancies and 6595 N-linked glycopeptides used a false discovery rate of less than 1%. Of 173 upregulated glycan in preterm group, we found low levels of fucosylation and high levels of sialylation in preterm birth (p < 0.05). Then we found that three poly-sialylated glycans had a high predictive value (AUC = 0.802, p < 0.017), which were expressed in all samples. In addition, the glycan model with clinical markers performed better. The results indicate that poly-sialylated glycans in CVF have potential value as novel clinical markers for predicting preterm birth during pregnancy. This study suggests strategies for developing new predictive biomarkers using cervicovaginal glycans to detect preterm birth in advance.https://doi.org/10.1038/s41598-025-96682-4Cervicovaginal fluidN-glycoproteomicPreterm birthPoly-sialylated glycanCommunity state type |
| spellingShingle | Yoon-Young Go Gun Wook Park Young Min Hur Young-Ah You Gain Lee Rin Chae Soo-Min Kim Sunwha Park Young Ju Kim Poly-sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birth Scientific Reports Cervicovaginal fluid N-glycoproteomic Preterm birth Poly-sialylated glycan Community state type |
| title | Poly-sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birth |
| title_full | Poly-sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birth |
| title_fullStr | Poly-sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birth |
| title_full_unstemmed | Poly-sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birth |
| title_short | Poly-sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birth |
| title_sort | poly sialylated glycan of cervicovaginal fluid can be a potential marker of preterm birth |
| topic | Cervicovaginal fluid N-glycoproteomic Preterm birth Poly-sialylated glycan Community state type |
| url | https://doi.org/10.1038/s41598-025-96682-4 |
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