Leaf characteristics and geographical distribution of Berberis amurensis and B. anhweiensis of Berberidaceae

Within the genus Berberis L., B. amurensis Rupr. and B. anhweiensis Ahrendt are easily confused in the main taxonomic characters, and the demarcation between these two species usually depends on the site information of their living plants or specimens.In order to further reveal their interspecific r...

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Main Authors: SHI Xiaoping, YUAN Sen, LI Xinhua, XING Guipei
Format: Article
Language:English
Published: Zhejiang University Press 2016-11-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2016.01.252
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author SHI Xiaoping
YUAN Sen
LI Xinhua
XING Guipei
author_facet SHI Xiaoping
YUAN Sen
LI Xinhua
XING Guipei
author_sort SHI Xiaoping
collection DOAJ
description Within the genus Berberis L., B. amurensis Rupr. and B. anhweiensis Ahrendt are easily confused in the main taxonomic characters, and the demarcation between these two species usually depends on the site information of their living plants or specimens.In order to further reveal their interspecific relationship, the leaf morphological and micromorphological features of B. amurensis and B. anhweiensis were studied by means of field surveys and experimental observation, and their geographical distribution patterns were also mapped and analyzed based on specimen check and literature analysis.The results showed that, although the leaf shape of both B. amurensis and B. anhweiensis varied greatly, continuous variation patterns still existed in both species, and obvious similarities were found between the two species in features of leaf shape, dense spinescent teeth on the leaf margin, and the reticulate venation. Light microscopy observation showed that, the outline of the periclinal walls of the leaf adaxial epidermal cells was approximately tetragonal to hexagonal in both B. amurensis and B. anhweiensis, but the anticlinal walls of the leaf adaxial epidermal cells were undulate or approximately straight in B. amurensis, and nearly straight or slightly undulate in B. anhweiensis. The outline of the periclinal walls of the leaf abaxial epidermal cells was irregularly polygonal in B. amurensis, and its anticlinal walls were undulate. The outline of the periclinal walls of the leaf abaxial epidermal cells was regularly polygonal in B. anhweiensis, and its anticlinal walls were nearly straight or slightly undulate. Both the two species were hypostomatic, and their stomata were all actinocytic and anomocytic. Scanning electron microscope observation revealed that, the leaf adaxial epidermis of B. amurensis was nearly smooth, or was covered with dense granular wax ornamentation, and the outline of the periclinal walls of the epidermal cells was irregularly polygonal, and the anticlinal walls were undulate; the leaf abaxial epidermis was nearly smooth, or was covered with dense granular wax ornamentation and remarkable bifurcate ridges. The leaf adaxial epidermis of B. anhweiensis was covered with dense fine granular wax ornamentation, and the outline of the periclinal walls of the epidermal cells was irregularly polygonal, and the anticlinal walls were undulate or approximately straight; the leaf abaxial epidermis was covered with dense granular or short filiform wax ornamentation and notable bifurcate ridges. The outline of the periclinal walls and the anticlinal walls of the leaf abaxial epidermal cells were all obscure in both B. amurensis and B. anhweiensis, and the inner margin of the outer stomatal ledges was nearly smooth or partially notched. There existed no obvious disjunctive geographical distribution between B. amurensis and B. anhweiensis in China, and the populations of B. amurensis might have extended its distribution from North China, via Qinling Mountains and Funiu Mountains, southward to Dabie Mountains, located across Central and East China, and spread closely to the distribution areas of B. anhweiensis in East China.Berberis anhweiensis was reported by AHRENDT in 1961, although AHRENDT considered that this species resembled B. chekiangensis Ahrendt; in fact, B. anhweiensis was much more similar to B. amurensis. Because of the considerable similarities between B. amurensis and B. anhweiensis in the morphological and micromorphological characteristics of leaves, together with the notable resemblances in other key taxonomic characters, e.g. inflorescence type and fruit morphology, which have been revealed by previous studies, and its continuous geographical distribution patterns, it is reasonable to treat B. anhweiensis as the synonymy of B. amurensis.
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spelling doaj-art-a140f570da444473984ef357350d7e4f2025-08-20T03:58:14ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552016-11-014268769310.3785/j.issn.1008-9209.2016.01.25210089209Leaf characteristics and geographical distribution of Berberis amurensis and B. anhweiensis of BerberidaceaeSHI XiaopingYUAN SenLI XinhuaXING GuipeiWithin the genus Berberis L., B. amurensis Rupr. and B. anhweiensis Ahrendt are easily confused in the main taxonomic characters, and the demarcation between these two species usually depends on the site information of their living plants or specimens.In order to further reveal their interspecific relationship, the leaf morphological and micromorphological features of B. amurensis and B. anhweiensis were studied by means of field surveys and experimental observation, and their geographical distribution patterns were also mapped and analyzed based on specimen check and literature analysis.The results showed that, although the leaf shape of both B. amurensis and B. anhweiensis varied greatly, continuous variation patterns still existed in both species, and obvious similarities were found between the two species in features of leaf shape, dense spinescent teeth on the leaf margin, and the reticulate venation. Light microscopy observation showed that, the outline of the periclinal walls of the leaf adaxial epidermal cells was approximately tetragonal to hexagonal in both B. amurensis and B. anhweiensis, but the anticlinal walls of the leaf adaxial epidermal cells were undulate or approximately straight in B. amurensis, and nearly straight or slightly undulate in B. anhweiensis. The outline of the periclinal walls of the leaf abaxial epidermal cells was irregularly polygonal in B. amurensis, and its anticlinal walls were undulate. The outline of the periclinal walls of the leaf abaxial epidermal cells was regularly polygonal in B. anhweiensis, and its anticlinal walls were nearly straight or slightly undulate. Both the two species were hypostomatic, and their stomata were all actinocytic and anomocytic. Scanning electron microscope observation revealed that, the leaf adaxial epidermis of B. amurensis was nearly smooth, or was covered with dense granular wax ornamentation, and the outline of the periclinal walls of the epidermal cells was irregularly polygonal, and the anticlinal walls were undulate; the leaf abaxial epidermis was nearly smooth, or was covered with dense granular wax ornamentation and remarkable bifurcate ridges. The leaf adaxial epidermis of B. anhweiensis was covered with dense fine granular wax ornamentation, and the outline of the periclinal walls of the epidermal cells was irregularly polygonal, and the anticlinal walls were undulate or approximately straight; the leaf abaxial epidermis was covered with dense granular or short filiform wax ornamentation and notable bifurcate ridges. The outline of the periclinal walls and the anticlinal walls of the leaf abaxial epidermal cells were all obscure in both B. amurensis and B. anhweiensis, and the inner margin of the outer stomatal ledges was nearly smooth or partially notched. There existed no obvious disjunctive geographical distribution between B. amurensis and B. anhweiensis in China, and the populations of B. amurensis might have extended its distribution from North China, via Qinling Mountains and Funiu Mountains, southward to Dabie Mountains, located across Central and East China, and spread closely to the distribution areas of B. anhweiensis in East China.Berberis anhweiensis was reported by AHRENDT in 1961, although AHRENDT considered that this species resembled B. chekiangensis Ahrendt; in fact, B. anhweiensis was much more similar to B. amurensis. Because of the considerable similarities between B. amurensis and B. anhweiensis in the morphological and micromorphological characteristics of leaves, together with the notable resemblances in other key taxonomic characters, e.g. inflorescence type and fruit morphology, which have been revealed by previous studies, and its continuous geographical distribution patterns, it is reasonable to treat B. anhweiensis as the synonymy of B. amurensis.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2016.01.252Berberis L.leafmorphologymicromorphologygeographical distributionnew synonymy
spellingShingle SHI Xiaoping
YUAN Sen
LI Xinhua
XING Guipei
Leaf characteristics and geographical distribution of Berberis amurensis and B. anhweiensis of Berberidaceae
浙江大学学报. 农业与生命科学版
Berberis L.
leaf
morphology
micromorphology
geographical distribution
new synonymy
title Leaf characteristics and geographical distribution of Berberis amurensis and B. anhweiensis of Berberidaceae
title_full Leaf characteristics and geographical distribution of Berberis amurensis and B. anhweiensis of Berberidaceae
title_fullStr Leaf characteristics and geographical distribution of Berberis amurensis and B. anhweiensis of Berberidaceae
title_full_unstemmed Leaf characteristics and geographical distribution of Berberis amurensis and B. anhweiensis of Berberidaceae
title_short Leaf characteristics and geographical distribution of Berberis amurensis and B. anhweiensis of Berberidaceae
title_sort leaf characteristics and geographical distribution of berberis amurensis and b anhweiensis of berberidaceae
topic Berberis L.
leaf
morphology
micromorphology
geographical distribution
new synonymy
url https://www.academax.com/doi/10.3785/j.issn.1008-9209.2016.01.252
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AT yuansen leafcharacteristicsandgeographicaldistributionofberberisamurensisandbanhweiensisofberberidaceae
AT lixinhua leafcharacteristicsandgeographicaldistributionofberberisamurensisandbanhweiensisofberberidaceae
AT xingguipei leafcharacteristicsandgeographicaldistributionofberberisamurensisandbanhweiensisofberberidaceae