Bioinformatics analysis of circular RNAs associated with atrial fibrillation and their evaluation as predictive biomarkers
Abstract Background Circular noncoding RNAs (circRNAs) are implicated in many human diseases, but their role in atrial fibrillation (AF) is poorly understood. In this study, we performed bioinformatics analysis of circRNA sequencing data to identify AF-related circRNAs. Methods Left atrial appendage...
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2025-05-01
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| Online Access: | https://doi.org/10.1186/s40246-025-00760-7 |
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| author | Manman Wang Yuanyuan Chen Weiwei Yang Xiangting Li Genli Liu Xin Wang Shuai Liu Ge Gao Fanhua Meng Feifei Kong Dandan Sun Wei Qin Bo Dong Jinguo Zhang |
| author_facet | Manman Wang Yuanyuan Chen Weiwei Yang Xiangting Li Genli Liu Xin Wang Shuai Liu Ge Gao Fanhua Meng Feifei Kong Dandan Sun Wei Qin Bo Dong Jinguo Zhang |
| author_sort | Manman Wang |
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| description | Abstract Background Circular noncoding RNAs (circRNAs) are implicated in many human diseases, but their role in atrial fibrillation (AF) is poorly understood. In this study, we performed bioinformatics analysis of circRNA sequencing data to identify AF-related circRNAs. Methods Left atrial appendage (LAA) samples were obtained from patients with valvular heart disease and were categorised into the sinus rhythm (SR; n = 4) and AF (n = 4) groups. CircRNA sequencing analysis was performed to identify differentially expressed (DE) circRNAs in AF patients. Functional enrichment analysis of DE circRNAs was performed to identify enriched Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Results We identified 3338 DE circRNAs, including 2147 upregulated and 1191 downregulated circRNAs, in AF patients. A ceRNA network of 16 DE circRNAs, 11 DE miRNAs, and 15 DE mRNAs was constructed. Functional enrichment analyses revealed that the AF-related DE circRNAs were enriched in response to vitamin D, the potassium channel complex, delayed rectifier potassium channel activity, osteoclast differentiation, primary immunodeficiency, endocrine and other factor-regulated calcium reabsorption and other processes. ROC curve analysis identified circRNA_00324, circRNA_17225, circRNA_16305, circRNA_10233, circRNA_05499, circRNA_03183, circRNA_14211, and circRNA_18422 as potential predictive biomarkers for distinguishing AF patients from SR patients, with AUC values of 0.9138, 0.7370, 0.8526, 0.6803, 0.8163, 0.8662, 0.7664, and 0.9320, respectively. Conclusions In this study, we constructed an AF-related ceRNA network and identified eight circRNAs as potential predictive biomarkers of AF. |
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| series | Human Genomics |
| spelling | doaj-art-a13a4f7fc68545f7a05fcf69d63bc1f72025-08-20T01:51:30ZengBMCHuman Genomics1479-73642025-05-0119111410.1186/s40246-025-00760-7Bioinformatics analysis of circular RNAs associated with atrial fibrillation and their evaluation as predictive biomarkersManman Wang0Yuanyuan Chen1Weiwei Yang2Xiangting Li3Genli Liu4Xin Wang5Shuai Liu6Ge Gao7Fanhua Meng8Feifei Kong9Dandan Sun10Wei Qin11Bo Dong12Jinguo Zhang13Shandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversityShandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversityDepartment of Medical Record, Affiliated Hospital of Jining Medical UniversityShandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversityShandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversityShandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversityShandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversityShandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversityShandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversityDepartment of Laboratory Medicine, Affiliated Hospital of Jining Medical UniversityShandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversitySchool of Pharmacy, Jining Medical UniversityDepartment of Cardiology, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityShandong Provincial Key Medical and Health Discipline of Cardiology, Jining Key Laboratory for Diagnosis and Treatment of Cardiovascular Diseases, Jining Key Laboratory of Precise Therapeutic Research of Coronary Intervention, Department of Cardiology, Affiliated Hospital of Jining Medical UniversityAbstract Background Circular noncoding RNAs (circRNAs) are implicated in many human diseases, but their role in atrial fibrillation (AF) is poorly understood. In this study, we performed bioinformatics analysis of circRNA sequencing data to identify AF-related circRNAs. Methods Left atrial appendage (LAA) samples were obtained from patients with valvular heart disease and were categorised into the sinus rhythm (SR; n = 4) and AF (n = 4) groups. CircRNA sequencing analysis was performed to identify differentially expressed (DE) circRNAs in AF patients. Functional enrichment analysis of DE circRNAs was performed to identify enriched Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Results We identified 3338 DE circRNAs, including 2147 upregulated and 1191 downregulated circRNAs, in AF patients. A ceRNA network of 16 DE circRNAs, 11 DE miRNAs, and 15 DE mRNAs was constructed. Functional enrichment analyses revealed that the AF-related DE circRNAs were enriched in response to vitamin D, the potassium channel complex, delayed rectifier potassium channel activity, osteoclast differentiation, primary immunodeficiency, endocrine and other factor-regulated calcium reabsorption and other processes. ROC curve analysis identified circRNA_00324, circRNA_17225, circRNA_16305, circRNA_10233, circRNA_05499, circRNA_03183, circRNA_14211, and circRNA_18422 as potential predictive biomarkers for distinguishing AF patients from SR patients, with AUC values of 0.9138, 0.7370, 0.8526, 0.6803, 0.8163, 0.8662, 0.7664, and 0.9320, respectively. Conclusions In this study, we constructed an AF-related ceRNA network and identified eight circRNAs as potential predictive biomarkers of AF.https://doi.org/10.1186/s40246-025-00760-7Atrial fibrillationCircular RNABioinformatic analysisCompeting endogenous RNABiomarker |
| spellingShingle | Manman Wang Yuanyuan Chen Weiwei Yang Xiangting Li Genli Liu Xin Wang Shuai Liu Ge Gao Fanhua Meng Feifei Kong Dandan Sun Wei Qin Bo Dong Jinguo Zhang Bioinformatics analysis of circular RNAs associated with atrial fibrillation and their evaluation as predictive biomarkers Human Genomics Atrial fibrillation Circular RNA Bioinformatic analysis Competing endogenous RNA Biomarker |
| title | Bioinformatics analysis of circular RNAs associated with atrial fibrillation and their evaluation as predictive biomarkers |
| title_full | Bioinformatics analysis of circular RNAs associated with atrial fibrillation and their evaluation as predictive biomarkers |
| title_fullStr | Bioinformatics analysis of circular RNAs associated with atrial fibrillation and their evaluation as predictive biomarkers |
| title_full_unstemmed | Bioinformatics analysis of circular RNAs associated with atrial fibrillation and their evaluation as predictive biomarkers |
| title_short | Bioinformatics analysis of circular RNAs associated with atrial fibrillation and their evaluation as predictive biomarkers |
| title_sort | bioinformatics analysis of circular rnas associated with atrial fibrillation and their evaluation as predictive biomarkers |
| topic | Atrial fibrillation Circular RNA Bioinformatic analysis Competing endogenous RNA Biomarker |
| url | https://doi.org/10.1186/s40246-025-00760-7 |
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