Evaluation of clinical impact of Interleukin 8 gene expression in patients with fibromyalgia

Abstract Background Fibromyalgia (FM) is a musculoskeletal syndrome characterized by diffuse and chronic pain associated with other symptoms such as fatigue, sleep/cognition disorders, headache, depression and anxiety, resulting from a change in pain processing. Previous research has shown an increa...

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Main Authors: Carolina Dias da Silva Amorim, Marco Felipe Macêdo Alves, Marcella Dias Teixeira, Larissa Vasconcelos Queiroz, Thaysa Walleria de Aragão Santos, Aline Ranzolin, Danyelly Bruneska Gondin Martins, Claudia Diniz Lopes Marques
Format: Article
Language:English
Published: BMC 2025-04-01
Series:Advances in Rheumatology
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Online Access:https://doi.org/10.1186/s42358-025-00453-8
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author Carolina Dias da Silva Amorim
Marco Felipe Macêdo Alves
Marcella Dias Teixeira
Larissa Vasconcelos Queiroz
Thaysa Walleria de Aragão Santos
Aline Ranzolin
Danyelly Bruneska Gondin Martins
Claudia Diniz Lopes Marques
author_facet Carolina Dias da Silva Amorim
Marco Felipe Macêdo Alves
Marcella Dias Teixeira
Larissa Vasconcelos Queiroz
Thaysa Walleria de Aragão Santos
Aline Ranzolin
Danyelly Bruneska Gondin Martins
Claudia Diniz Lopes Marques
author_sort Carolina Dias da Silva Amorim
collection DOAJ
description Abstract Background Fibromyalgia (FM) is a musculoskeletal syndrome characterized by diffuse and chronic pain associated with other symptoms such as fatigue, sleep/cognition disorders, headache, depression and anxiety, resulting from a change in pain processing. Previous research has shown an increase in some interleukins (IL) in patients with FM when compared to controls, however, there is still no uniformity and consensus in the results. There is no study that evaluates IL8 mRNA expression in FM and its association with obesity and other clinical parameters. This study aims to verify the impact of IL8 mRNA expression on the clinical parameters of patients with FM (FMG) in relation to the comparison group (CG). Method This study evaluated patients diagnosed with FM treated at the rheumatology service of the Hospital das Clínicas of the Federal University of Pernambuco (HC-UFPE). The CG group was composed of individuals without chronic pain (companions of the patients and hospital employees). Clinical and demographic data were collected in both groups, and questionnaires for fatigue (FACIT-F), impact of FM (FIQ-R), depression (BDI), and sleep (NRS) were applied to both groups. Peripheral blood was collected for evaluation of IL8 gene expression through real time polymerase chain reaction (qPCR). Results Patients with FM show a lower frequency of IL8 gene expression compared to the CG, but FMG presented mainly up regulated in relation to CG. There was no association of IL8 expression and worse FIQ-R indices, sleep disturbance, BMI or fatigue. However, there was an association between IL8 expression and moderate depression (p = 0.002) and physical activity (p = 0.039), where patients in FMG who did express IL8 were practicing less physical activity. Conclusion Patients in FMG did not have a higher frequency of IL8 expression compared to CG, however patients with IL8 expression have a greater association with moderate depression.
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spelling doaj-art-a13a1c6dca074769b7624488ffada4632025-08-20T03:06:36ZengBMCAdvances in Rheumatology2523-31062025-04-0165111010.1186/s42358-025-00453-8Evaluation of clinical impact of Interleukin 8 gene expression in patients with fibromyalgiaCarolina Dias da Silva Amorim0Marco Felipe Macêdo Alves1Marcella Dias Teixeira2Larissa Vasconcelos Queiroz3Thaysa Walleria de Aragão Santos4Aline Ranzolin5Danyelly Bruneska Gondin Martins6Claudia Diniz Lopes Marques7Programa de pós-graduação em Saúde Translacional, Centro de Ciências Médicas, Universidade Federal de PernambucoPrograma de residência médica em reumatologia do Hospital das Clínicas/Ebserh, Universidade Federal de PernambucoPrograma de residência médica em reumatologia do Hospital das Clínicas/Ebserh, Universidade Federal de PernambucoPrograma de residência médica em reumatologia do Hospital das Clínicas/Ebserh, Universidade Federal de PernambucoInstituto Keizo Asami (iLIKA), Centro de Biociências, Universidade Federal de PernambucoHospital das Clínicas, Universidade Federal de Pernambuco, Empresa Brasileira de Serviços Hospitalares (Ebserh)Instituto Keizo Asami (iLIKA), Centro de Biociências, Universidade Federal de PernambucoHospital das Clínicas, Universidade Federal de Pernambuco, Empresa Brasileira de Serviços Hospitalares (Ebserh)Abstract Background Fibromyalgia (FM) is a musculoskeletal syndrome characterized by diffuse and chronic pain associated with other symptoms such as fatigue, sleep/cognition disorders, headache, depression and anxiety, resulting from a change in pain processing. Previous research has shown an increase in some interleukins (IL) in patients with FM when compared to controls, however, there is still no uniformity and consensus in the results. There is no study that evaluates IL8 mRNA expression in FM and its association with obesity and other clinical parameters. This study aims to verify the impact of IL8 mRNA expression on the clinical parameters of patients with FM (FMG) in relation to the comparison group (CG). Method This study evaluated patients diagnosed with FM treated at the rheumatology service of the Hospital das Clínicas of the Federal University of Pernambuco (HC-UFPE). The CG group was composed of individuals without chronic pain (companions of the patients and hospital employees). Clinical and demographic data were collected in both groups, and questionnaires for fatigue (FACIT-F), impact of FM (FIQ-R), depression (BDI), and sleep (NRS) were applied to both groups. Peripheral blood was collected for evaluation of IL8 gene expression through real time polymerase chain reaction (qPCR). Results Patients with FM show a lower frequency of IL8 gene expression compared to the CG, but FMG presented mainly up regulated in relation to CG. There was no association of IL8 expression and worse FIQ-R indices, sleep disturbance, BMI or fatigue. However, there was an association between IL8 expression and moderate depression (p = 0.002) and physical activity (p = 0.039), where patients in FMG who did express IL8 were practicing less physical activity. Conclusion Patients in FMG did not have a higher frequency of IL8 expression compared to CG, however patients with IL8 expression have a greater association with moderate depression.https://doi.org/10.1186/s42358-025-00453-8FibromyalgiaInterleukin 8DepressionFatigueSleep qualityObesity
spellingShingle Carolina Dias da Silva Amorim
Marco Felipe Macêdo Alves
Marcella Dias Teixeira
Larissa Vasconcelos Queiroz
Thaysa Walleria de Aragão Santos
Aline Ranzolin
Danyelly Bruneska Gondin Martins
Claudia Diniz Lopes Marques
Evaluation of clinical impact of Interleukin 8 gene expression in patients with fibromyalgia
Advances in Rheumatology
Fibromyalgia
Interleukin 8
Depression
Fatigue
Sleep quality
Obesity
title Evaluation of clinical impact of Interleukin 8 gene expression in patients with fibromyalgia
title_full Evaluation of clinical impact of Interleukin 8 gene expression in patients with fibromyalgia
title_fullStr Evaluation of clinical impact of Interleukin 8 gene expression in patients with fibromyalgia
title_full_unstemmed Evaluation of clinical impact of Interleukin 8 gene expression in patients with fibromyalgia
title_short Evaluation of clinical impact of Interleukin 8 gene expression in patients with fibromyalgia
title_sort evaluation of clinical impact of interleukin 8 gene expression in patients with fibromyalgia
topic Fibromyalgia
Interleukin 8
Depression
Fatigue
Sleep quality
Obesity
url https://doi.org/10.1186/s42358-025-00453-8
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