Influence of the purification of human adult pancreatic islets on insulin secretion
Background/Aim. The most effective method for human adult pancreatic islets purification is density-gradient centrifugation. The aim of this study was to analyze the effects of non-automated purification on preservation of functional capacity of human adult pancreatic islet cells. Methods. Human pan...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2010-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2010/0042-84501002128N.pdf |
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| author | Nikolić Dragan M. Đorđević Predrag B. Dimitrijević-Srećković Vesna Džingalašević Marina Belij Sandra Kalezić Nevena |
| author_facet | Nikolić Dragan M. Đorđević Predrag B. Dimitrijević-Srećković Vesna Džingalašević Marina Belij Sandra Kalezić Nevena |
| author_sort | Nikolić Dragan M. |
| collection | DOAJ |
| description | Background/Aim. The most effective method for human adult pancreatic islets purification is density-gradient centrifugation. The aim of this study was to analyze the effects of non-automated purification on preservation of functional capacity of human adult pancreatic islet cells. Methods. Human pancreata were obtained after pancreatectomy in the patients with chronic pancreatitis or benign tumors. Pancreatic islets were purified by non-automated method in discontinuous Ficoll density gradient. The samples were divided in 2 fractions: purified (P) and non-purified (NP) cultures. Islets were stained with diphenyl-thiocarbazone. The efficiency of separation was determined by comparing percentage of stained cells in P and NP cultures on day 1, 3 and 7 of shortterm cultivation. Glucose-stimulated insulin secretion was expressed as stimulation index (SI). Results. The results obtained showed a statistically significant difference (p < 0.01) between P and NP cultures. P cultures had higher percentages of stained cells (70.43 ± 3.97%, 73.77 ± 4.22% and 71.34 ± 4.69% on the first, third and seventh day of cultivation, respectively) than NP cultures (53.68 ± 1.71%, 57.14 ± 3.94% and 43.97 ± 4.56%, respectively). P cultures had higher values of SI for the first, third and seventh day of cultivation than NP cultures (0.45 ± 0.08, 0.80 ± 0.21, 1.28 ± 0.15 and 0.46 ± 0.10, 0.752 ± .0.16, 0.76 ± 0.11 for P and NP cultures respectively). The difference was statistically significant on day seven (p = 0.01). Conclusion. Although during purification process islets were exposed to a number of insults that might result in cellular damage and functional impairment, our assessments showed that islets in P cultures preserved their functional capacity better than islets in NP cultures, since they had greater insulin secretion. |
| format | Article |
| id | doaj-art-a13576f4ee4143399dd19ee87c5fb4d0 |
| institution | OA Journals |
| issn | 0042-8450 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-a13576f4ee4143399dd19ee87c5fb4d02025-08-20T02:20:51ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502010-01-0167212813110.2298/VSP1002128NInfluence of the purification of human adult pancreatic islets on insulin secretionNikolić Dragan M.Đorđević Predrag B.Dimitrijević-Srećković VesnaDžingalašević MarinaBelij SandraKalezić NevenaBackground/Aim. The most effective method for human adult pancreatic islets purification is density-gradient centrifugation. The aim of this study was to analyze the effects of non-automated purification on preservation of functional capacity of human adult pancreatic islet cells. Methods. Human pancreata were obtained after pancreatectomy in the patients with chronic pancreatitis or benign tumors. Pancreatic islets were purified by non-automated method in discontinuous Ficoll density gradient. The samples were divided in 2 fractions: purified (P) and non-purified (NP) cultures. Islets were stained with diphenyl-thiocarbazone. The efficiency of separation was determined by comparing percentage of stained cells in P and NP cultures on day 1, 3 and 7 of shortterm cultivation. Glucose-stimulated insulin secretion was expressed as stimulation index (SI). Results. The results obtained showed a statistically significant difference (p < 0.01) between P and NP cultures. P cultures had higher percentages of stained cells (70.43 ± 3.97%, 73.77 ± 4.22% and 71.34 ± 4.69% on the first, third and seventh day of cultivation, respectively) than NP cultures (53.68 ± 1.71%, 57.14 ± 3.94% and 43.97 ± 4.56%, respectively). P cultures had higher values of SI for the first, third and seventh day of cultivation than NP cultures (0.45 ± 0.08, 0.80 ± 0.21, 1.28 ± 0.15 and 0.46 ± 0.10, 0.752 ± .0.16, 0.76 ± 0.11 for P and NP cultures respectively). The difference was statistically significant on day seven (p = 0.01). Conclusion. Although during purification process islets were exposed to a number of insults that might result in cellular damage and functional impairment, our assessments showed that islets in P cultures preserved their functional capacity better than islets in NP cultures, since they had greater insulin secretion.http://www.doiserbia.nb.rs/img/doi/0042-8450/2010/0042-84501002128N.pdfislets of langerhans transplantationinsulinsecretory pathway |
| spellingShingle | Nikolić Dragan M. Đorđević Predrag B. Dimitrijević-Srećković Vesna Džingalašević Marina Belij Sandra Kalezić Nevena Influence of the purification of human adult pancreatic islets on insulin secretion Vojnosanitetski Pregled islets of langerhans transplantation insulin secretory pathway |
| title | Influence of the purification of human adult pancreatic islets on insulin secretion |
| title_full | Influence of the purification of human adult pancreatic islets on insulin secretion |
| title_fullStr | Influence of the purification of human adult pancreatic islets on insulin secretion |
| title_full_unstemmed | Influence of the purification of human adult pancreatic islets on insulin secretion |
| title_short | Influence of the purification of human adult pancreatic islets on insulin secretion |
| title_sort | influence of the purification of human adult pancreatic islets on insulin secretion |
| topic | islets of langerhans transplantation insulin secretory pathway |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2010/0042-84501002128N.pdf |
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