The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation
Background. DNA demethylation, the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), plays important roles in diverse biological processes and multiple diseases by regulating gene expression. Methods. In this study, utilizi...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/2186301 |
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| author | Huikang Tao Pei Xie Yuhang Cao Liqi Shu Liping Li Junchen Chen Guangfeng Tian Yingliang Zhuang Qiang Shu Xuekun Li |
| author_facet | Huikang Tao Pei Xie Yuhang Cao Liqi Shu Liping Li Junchen Chen Guangfeng Tian Yingliang Zhuang Qiang Shu Xuekun Li |
| author_sort | Huikang Tao |
| collection | DOAJ |
| description | Background. DNA demethylation, the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), plays important roles in diverse biological processes and multiple diseases by regulating gene expression. Methods. In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice. Results. It was found that 5hmC, 5fC, and 5caC were highly enriched in multiple brain regions and aNSCs and displayed temporal and spatial patterns during postnatal neuronal development and the differentiation of aNSCs. Consistently, the expression of Tets also exhibited temporal and spatial patterns. Conclusion. DNA demethylation displayed dynamic features during postnatal neuronal development and the differentiation of aNSCs of mice, which could contribute to appropriate gene expression. |
| format | Article |
| id | doaj-art-a130ab2ee6894f90b121f29513d1e5d9 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-a130ab2ee6894f90b121f29513d1e5d92025-02-03T05:52:32ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/21863012186301The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell DifferentiationHuikang Tao0Pei Xie1Yuhang Cao2Liqi Shu3Liping Li4Junchen Chen5Guangfeng Tian6Yingliang Zhuang7Qiang Shu8Xuekun Li9The Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaSchool of Medicine and Health Sciences, George Washington University, Washington, DC 20037, USAThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaThe Children’s Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, ChinaBackground. DNA demethylation, the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), plays important roles in diverse biological processes and multiple diseases by regulating gene expression. Methods. In this study, utilizing DNA dot blot, immunofluorescence staining, and qRT-PCR, we studied the expression pattern of Tets, the enzymes governing DNA demethylation, and the levels of 5hmC, 5fC, and 5caC during the postnatal neuronal development of mice. Results. It was found that 5hmC, 5fC, and 5caC were highly enriched in multiple brain regions and aNSCs and displayed temporal and spatial patterns during postnatal neuronal development and the differentiation of aNSCs. Consistently, the expression of Tets also exhibited temporal and spatial patterns. Conclusion. DNA demethylation displayed dynamic features during postnatal neuronal development and the differentiation of aNSCs of mice, which could contribute to appropriate gene expression.http://dx.doi.org/10.1155/2018/2186301 |
| spellingShingle | Huikang Tao Pei Xie Yuhang Cao Liqi Shu Liping Li Junchen Chen Guangfeng Tian Yingliang Zhuang Qiang Shu Xuekun Li The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation Stem Cells International |
| title | The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation |
| title_full | The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation |
| title_fullStr | The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation |
| title_full_unstemmed | The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation |
| title_short | The Dynamic DNA Demethylation during Postnatal Neuronal Development and Neural Stem Cell Differentiation |
| title_sort | dynamic dna demethylation during postnatal neuronal development and neural stem cell differentiation |
| url | http://dx.doi.org/10.1155/2018/2186301 |
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