Transcriptomic network underlying physiological alterations in the stem of Myricaria laxiflora in response to waterlogging stress
Myricaria laxiflora is an endangered shrub plant with remarkable tolerance to waterlogging stress, however, little attention has been paid to understanding the underlying mechanisms. Here, physiological and transcriptomic approaches were applied to uncover the physiological and molecular reconfigura...
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Elsevier
2024-10-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324010674 |
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| author | Linbao Li Yang Su Weibo Xiang Guiyun Huang Qianyan Liang Bicheng Dun Haibo Zhang Zhiqiang Xiao Liwen Qiu Jun Zhang Di Wu |
| author_facet | Linbao Li Yang Su Weibo Xiang Guiyun Huang Qianyan Liang Bicheng Dun Haibo Zhang Zhiqiang Xiao Liwen Qiu Jun Zhang Di Wu |
| author_sort | Linbao Li |
| collection | DOAJ |
| description | Myricaria laxiflora is an endangered shrub plant with remarkable tolerance to waterlogging stress, however, little attention has been paid to understanding the underlying mechanisms. Here, physiological and transcriptomic approaches were applied to uncover the physiological and molecular reconfigurations in the stem of M. laxiflora in response to waterlogging stress. The accumulation of the contents of H2O2 and malonaldehyde (MDA) alongside increased activities of enzymes for scavenging the reactive oxygen species (ROS) in the stem of M. laxiflora were observed under waterlogging stress. The principal component analysis (PCA) of transcriptomes from five different timepoints uncovered PC1 counted for 17.3 % of total variations and separated the treated and non-treated samples. A total of 8714 genes in the stem of M. laxiflora were identified as differentially expressed genes (DEGs) under waterlogging stress, which could be assigned into two different subgroups with distinct gene expression patterns and biological functions. The DEGs involved in glycolysis were generally upregulated, whereas opposite results were observed for nitrogen uptake and the assimilation pathway. The contents of abscisic acid (ABA) and jasmonic acid (JA) were sharply decreased alongside the decreased mRNA levels of the genes involved in corresponding synthesis pathways upon waterlogging stress. A network centered by eight key transcription factors has been constructed, which uncovered the inhibited cell division processes in the stem of M. laxiflora upon waterlogging stress. Taken together, the obtained results showed that glycolysis, nitrogen metabolism and meristem activities played an important role in the stem of M. laxiflora in response to waterlogging stress. |
| format | Article |
| id | doaj-art-a12311af45f74d078a1649fbdfb5c8be |
| institution | OA Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-a12311af45f74d078a1649fbdfb5c8be2025-08-20T01:54:44ZengElsevierEcotoxicology and Environmental Safety0147-65132024-10-0128411699110.1016/j.ecoenv.2024.116991Transcriptomic network underlying physiological alterations in the stem of Myricaria laxiflora in response to waterlogging stressLinbao Li0Yang Su1Weibo Xiang2Guiyun Huang3Qianyan Liang4Bicheng Dun5Haibo Zhang6Zhiqiang Xiao7Liwen Qiu8Jun Zhang9Di Wu10Yangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, ChinaYangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, China; Hubei Key Laboratory of Rare Resource Plants in Three Gorges Reservoir Area, Yichang 443100, China; National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt, China Three Gorges Corporation, Wuhan 100083, China; Corresponding author at: Yangtze River Biodiversity Research Centre, China Three Gorges Corporation, Wuhan 443133, ChinaMyricaria laxiflora is an endangered shrub plant with remarkable tolerance to waterlogging stress, however, little attention has been paid to understanding the underlying mechanisms. Here, physiological and transcriptomic approaches were applied to uncover the physiological and molecular reconfigurations in the stem of M. laxiflora in response to waterlogging stress. The accumulation of the contents of H2O2 and malonaldehyde (MDA) alongside increased activities of enzymes for scavenging the reactive oxygen species (ROS) in the stem of M. laxiflora were observed under waterlogging stress. The principal component analysis (PCA) of transcriptomes from five different timepoints uncovered PC1 counted for 17.3 % of total variations and separated the treated and non-treated samples. A total of 8714 genes in the stem of M. laxiflora were identified as differentially expressed genes (DEGs) under waterlogging stress, which could be assigned into two different subgroups with distinct gene expression patterns and biological functions. The DEGs involved in glycolysis were generally upregulated, whereas opposite results were observed for nitrogen uptake and the assimilation pathway. The contents of abscisic acid (ABA) and jasmonic acid (JA) were sharply decreased alongside the decreased mRNA levels of the genes involved in corresponding synthesis pathways upon waterlogging stress. A network centered by eight key transcription factors has been constructed, which uncovered the inhibited cell division processes in the stem of M. laxiflora upon waterlogging stress. Taken together, the obtained results showed that glycolysis, nitrogen metabolism and meristem activities played an important role in the stem of M. laxiflora in response to waterlogging stress.http://www.sciencedirect.com/science/article/pii/S0147651324010674Co-expression networkMyricaria laxifloraPhytohormoneRNA-seqWaterlogging stress |
| spellingShingle | Linbao Li Yang Su Weibo Xiang Guiyun Huang Qianyan Liang Bicheng Dun Haibo Zhang Zhiqiang Xiao Liwen Qiu Jun Zhang Di Wu Transcriptomic network underlying physiological alterations in the stem of Myricaria laxiflora in response to waterlogging stress Ecotoxicology and Environmental Safety Co-expression network Myricaria laxiflora Phytohormone RNA-seq Waterlogging stress |
| title | Transcriptomic network underlying physiological alterations in the stem of Myricaria laxiflora in response to waterlogging stress |
| title_full | Transcriptomic network underlying physiological alterations in the stem of Myricaria laxiflora in response to waterlogging stress |
| title_fullStr | Transcriptomic network underlying physiological alterations in the stem of Myricaria laxiflora in response to waterlogging stress |
| title_full_unstemmed | Transcriptomic network underlying physiological alterations in the stem of Myricaria laxiflora in response to waterlogging stress |
| title_short | Transcriptomic network underlying physiological alterations in the stem of Myricaria laxiflora in response to waterlogging stress |
| title_sort | transcriptomic network underlying physiological alterations in the stem of myricaria laxiflora in response to waterlogging stress |
| topic | Co-expression network Myricaria laxiflora Phytohormone RNA-seq Waterlogging stress |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324010674 |
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