The Potential Effect of Bualuang (White <i>Nelumbo nucifera</i> Gaertn.) Extract on Sperm Quality and Metabolomic Profiles in Mancozeb-Induced Oxidative Stress in Male Rats
Mancozeb (MZ), an EBDC fungicide, has been found to contaminate agricultural products and cause male reproductive toxicity. The phytochemical compounds of white <i>N. nucifera</i> petal extract (WNPE) and its effects on metabolomic profiles and reproductive function in male rats poisoned...
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2024-12-01
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| author | Jiraporn Laoung-on Pimchanok Nuchniyom Ketsarin Intui Churdsak Jaikang Kanokporn Saenphet Kongsak Boonyapranai Giatgong Konguthaithip Nopparuj Outaitaveep Sasitorn Phankhieo Paiwan Sudwan |
| author_facet | Jiraporn Laoung-on Pimchanok Nuchniyom Ketsarin Intui Churdsak Jaikang Kanokporn Saenphet Kongsak Boonyapranai Giatgong Konguthaithip Nopparuj Outaitaveep Sasitorn Phankhieo Paiwan Sudwan |
| author_sort | Jiraporn Laoung-on |
| collection | DOAJ |
| description | Mancozeb (MZ), an EBDC fungicide, has been found to contaminate agricultural products and cause male reproductive toxicity. The phytochemical compounds of white <i>N. nucifera</i> petal extract (WNPE) and its effects on metabolomic profiles and reproductive function in male rats poisoned with MZ were investigated. Seventy-two mature male Wistar rats were divided into nine groups (<i>n</i> = 8) and, for 30 days, were gavaged with WNPE at doses of 0.55, 1.10, and 2.20 mg/kg; were given distilled water; or were co-gavaged with MZ and WNPE. By evaluating the <sup>1</sup>H-NMR of WNPE, myricetin, apigenin, luteolin, ferulic acid, caffeic acid, ascorbic acid, genistein, chlorogenic acid, naringenin, and ellagic acid were found, and the essential minerals were evaluated by AAS. The NMR spectra demonstrated that creatine, carnitine, ACh, and choline in WNPE were significantly higher than that in MZ. The gavaging of the rats with WNPE before poisoning them with MZ improved creatine, carnitine, acetylcholine, progressive sperm motility, sperm viability, and normal sperm morphology compared to rats who only received MZ. It was concluded that MZ had a toxicity effect on the male reproductive system via decreased metabolomic profiles, affecting sperm motility, sperm viability, and normal sperm morphology. Nevertheless, WNPE had plenty of bioactive compounds that could enhance creatine, carnitine, and acetylcholine, which are related to sperm quality in male rats. WNPE should be considered as an alternative dietary supplement that can protect against MZ toxicity and enhance sperm quality in the male rat reproductive system. |
| format | Article |
| id | doaj-art-a11dd8a6ac404f269a05e947fe069572 |
| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Life |
| spelling | doaj-art-a11dd8a6ac404f269a05e947fe0695722025-01-24T13:38:25ZengMDPI AGLife2075-17292024-12-01151610.3390/life15010006The Potential Effect of Bualuang (White <i>Nelumbo nucifera</i> Gaertn.) Extract on Sperm Quality and Metabolomic Profiles in Mancozeb-Induced Oxidative Stress in Male RatsJiraporn Laoung-on0Pimchanok Nuchniyom1Ketsarin Intui2Churdsak Jaikang3Kanokporn Saenphet4Kongsak Boonyapranai5Giatgong Konguthaithip6Nopparuj Outaitaveep7Sasitorn Phankhieo8Paiwan Sudwan9Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandToxicology Section, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandResearch Institute for Health Sciences (RIHES), Chiang Mai University, Chiang Mai 50200, ThailandToxicology Section, Department of Forensic Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandSchool of Health Sciences Research, Research Institute for Health Sciences (RIHES), Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandMancozeb (MZ), an EBDC fungicide, has been found to contaminate agricultural products and cause male reproductive toxicity. The phytochemical compounds of white <i>N. nucifera</i> petal extract (WNPE) and its effects on metabolomic profiles and reproductive function in male rats poisoned with MZ were investigated. Seventy-two mature male Wistar rats were divided into nine groups (<i>n</i> = 8) and, for 30 days, were gavaged with WNPE at doses of 0.55, 1.10, and 2.20 mg/kg; were given distilled water; or were co-gavaged with MZ and WNPE. By evaluating the <sup>1</sup>H-NMR of WNPE, myricetin, apigenin, luteolin, ferulic acid, caffeic acid, ascorbic acid, genistein, chlorogenic acid, naringenin, and ellagic acid were found, and the essential minerals were evaluated by AAS. The NMR spectra demonstrated that creatine, carnitine, ACh, and choline in WNPE were significantly higher than that in MZ. The gavaging of the rats with WNPE before poisoning them with MZ improved creatine, carnitine, acetylcholine, progressive sperm motility, sperm viability, and normal sperm morphology compared to rats who only received MZ. It was concluded that MZ had a toxicity effect on the male reproductive system via decreased metabolomic profiles, affecting sperm motility, sperm viability, and normal sperm morphology. Nevertheless, WNPE had plenty of bioactive compounds that could enhance creatine, carnitine, and acetylcholine, which are related to sperm quality in male rats. WNPE should be considered as an alternative dietary supplement that can protect against MZ toxicity and enhance sperm quality in the male rat reproductive system.https://www.mdpi.com/2075-1729/15/1/6environment toxicantoxidative stress<i>Nelumbo nucifera</i> petalmale reproductive health<sup>1</sup>H-NMRsperm |
| spellingShingle | Jiraporn Laoung-on Pimchanok Nuchniyom Ketsarin Intui Churdsak Jaikang Kanokporn Saenphet Kongsak Boonyapranai Giatgong Konguthaithip Nopparuj Outaitaveep Sasitorn Phankhieo Paiwan Sudwan The Potential Effect of Bualuang (White <i>Nelumbo nucifera</i> Gaertn.) Extract on Sperm Quality and Metabolomic Profiles in Mancozeb-Induced Oxidative Stress in Male Rats Life environment toxicant oxidative stress <i>Nelumbo nucifera</i> petal male reproductive health <sup>1</sup>H-NMR sperm |
| title | The Potential Effect of Bualuang (White <i>Nelumbo nucifera</i> Gaertn.) Extract on Sperm Quality and Metabolomic Profiles in Mancozeb-Induced Oxidative Stress in Male Rats |
| title_full | The Potential Effect of Bualuang (White <i>Nelumbo nucifera</i> Gaertn.) Extract on Sperm Quality and Metabolomic Profiles in Mancozeb-Induced Oxidative Stress in Male Rats |
| title_fullStr | The Potential Effect of Bualuang (White <i>Nelumbo nucifera</i> Gaertn.) Extract on Sperm Quality and Metabolomic Profiles in Mancozeb-Induced Oxidative Stress in Male Rats |
| title_full_unstemmed | The Potential Effect of Bualuang (White <i>Nelumbo nucifera</i> Gaertn.) Extract on Sperm Quality and Metabolomic Profiles in Mancozeb-Induced Oxidative Stress in Male Rats |
| title_short | The Potential Effect of Bualuang (White <i>Nelumbo nucifera</i> Gaertn.) Extract on Sperm Quality and Metabolomic Profiles in Mancozeb-Induced Oxidative Stress in Male Rats |
| title_sort | potential effect of bualuang white i nelumbo nucifera i gaertn extract on sperm quality and metabolomic profiles in mancozeb induced oxidative stress in male rats |
| topic | environment toxicant oxidative stress <i>Nelumbo nucifera</i> petal male reproductive health <sup>1</sup>H-NMR sperm |
| url | https://www.mdpi.com/2075-1729/15/1/6 |
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