High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single Run
This paper reports the development of a subminute separation method by capillary zone electrophoresis in an uncoated capillary using multiple injection procedure for the determination of lidocaine in samples of pharmaceutical formulations. The separation was performed in less than a minute leading t...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2016/4126810 |
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| author | Andressa C. Valese Daniel A. Spudeit Maressa D. Dolzan Lizandra C. Bretanha Luciano Vitali Gustavo A. Micke |
| author_facet | Andressa C. Valese Daniel A. Spudeit Maressa D. Dolzan Lizandra C. Bretanha Luciano Vitali Gustavo A. Micke |
| author_sort | Andressa C. Valese |
| collection | DOAJ |
| description | This paper reports the development of a subminute separation method by capillary zone electrophoresis in an uncoated capillary using multiple injection procedure for the determination of lidocaine in samples of pharmaceutical formulations. The separation was performed in less than a minute leading to doing four injections in a single run. The cathodic electroosmotic flow contributed to reducing the analyses time. The background electrolyte was composed of 20 mmol L−1 2-amino-2-(hydroxymethyl)-1,3-propanediol and 40 mmol L−1 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The internal standard used was benzylamine. Separations were performed in a fused uncoated silica capillary (32 cm total length, 23.5 cm effective length, and 50 μm internal diameter) with direct UV detection at 200 nm. Samples and standards were injected hydrodynamically using 40 mbar/3 s interspersed with spacer electrolyte using 40 mbar/7 s. The electrophoretic system was operated under constant voltage of 30 kV with positive polarity on the injection side. The evaluation of some analytical parameters of the method showed good linearity (r2>0.999), a limit of detection 0.92 mg L−1, intermediate precision better than 3.2% (peak area), and recovery in the range of 92–102%. |
| format | Article |
| id | doaj-art-a11d1b9298ff47119e4a1fcd8ec5354e |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-a11d1b9298ff47119e4a1fcd8ec5354e2025-08-20T03:38:34ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732016-01-01201610.1155/2016/41268104126810High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single RunAndressa C. Valese0Daniel A. Spudeit1Maressa D. Dolzan2Lizandra C. Bretanha3Luciano Vitali4Gustavo A. Micke5Department of Food Science Technologies, Federal University of Santa Catarina, Florianopolis, SC, BrazilDepartamento de Química, CFM, UFSC, Campus Universitário, Trindade, CP 476, 88040-900 Florianópolis, SC, BrazilDepartamento de Química, CFM, UFSC, Campus Universitário, Trindade, CP 476, 88040-900 Florianópolis, SC, BrazilDepartment of Food Science Technologies, Federal University of Santa Catarina, Florianopolis, SC, BrazilDepartamento de Química, CFM, UFSC, Campus Universitário, Trindade, CP 476, 88040-900 Florianópolis, SC, BrazilDepartamento de Química, CFM, UFSC, Campus Universitário, Trindade, CP 476, 88040-900 Florianópolis, SC, BrazilThis paper reports the development of a subminute separation method by capillary zone electrophoresis in an uncoated capillary using multiple injection procedure for the determination of lidocaine in samples of pharmaceutical formulations. The separation was performed in less than a minute leading to doing four injections in a single run. The cathodic electroosmotic flow contributed to reducing the analyses time. The background electrolyte was composed of 20 mmol L−1 2-amino-2-(hydroxymethyl)-1,3-propanediol and 40 mmol L−1 2-(N-morpholino)ethanesulfonic acid at pH 6.1. The internal standard used was benzylamine. Separations were performed in a fused uncoated silica capillary (32 cm total length, 23.5 cm effective length, and 50 μm internal diameter) with direct UV detection at 200 nm. Samples and standards were injected hydrodynamically using 40 mbar/3 s interspersed with spacer electrolyte using 40 mbar/7 s. The electrophoretic system was operated under constant voltage of 30 kV with positive polarity on the injection side. The evaluation of some analytical parameters of the method showed good linearity (r2>0.999), a limit of detection 0.92 mg L−1, intermediate precision better than 3.2% (peak area), and recovery in the range of 92–102%.http://dx.doi.org/10.1155/2016/4126810 |
| spellingShingle | Andressa C. Valese Daniel A. Spudeit Maressa D. Dolzan Lizandra C. Bretanha Luciano Vitali Gustavo A. Micke High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single Run Journal of Analytical Methods in Chemistry |
| title | High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single Run |
| title_full | High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single Run |
| title_fullStr | High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single Run |
| title_full_unstemmed | High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single Run |
| title_short | High-Throughput Analysis of Lidocaine in Pharmaceutical Formulation by Capillary Zone Electrophoresis Using Multiple Injections in a Single Run |
| title_sort | high throughput analysis of lidocaine in pharmaceutical formulation by capillary zone electrophoresis using multiple injections in a single run |
| url | http://dx.doi.org/10.1155/2016/4126810 |
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