ggClusterNet 2: An R package for microbial co‐occurrence networks and associated indicator correlation patterns

Abstract Since its initial release in 2022, ggClusterNet has become a vital tool for microbiome research, enabling microbial co‐occurrence network analysis and visualization in over 300 studies. To address emerging challenges, including multi‐factor experimental designs, multi‐treatment conditions,...

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Main Authors: Tao Wen, Yong‐Xin Liu, Lanlan Liu, Guoqing Niu, Zhexu Ding, Xinyang Teng, Jie Ma, Ying Liu, Shengdie Yang, Penghao Xie, Tianjiao Zhang, Lei Wang, Zhanyuan Lu, Qirong Shen, Jun Yuan
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:iMeta
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Online Access:https://doi.org/10.1002/imt2.70041
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author Tao Wen
Yong‐Xin Liu
Lanlan Liu
Guoqing Niu
Zhexu Ding
Xinyang Teng
Jie Ma
Ying Liu
Shengdie Yang
Penghao Xie
Tianjiao Zhang
Lei Wang
Zhanyuan Lu
Qirong Shen
Jun Yuan
author_facet Tao Wen
Yong‐Xin Liu
Lanlan Liu
Guoqing Niu
Zhexu Ding
Xinyang Teng
Jie Ma
Ying Liu
Shengdie Yang
Penghao Xie
Tianjiao Zhang
Lei Wang
Zhanyuan Lu
Qirong Shen
Jun Yuan
author_sort Tao Wen
collection DOAJ
description Abstract Since its initial release in 2022, ggClusterNet has become a vital tool for microbiome research, enabling microbial co‐occurrence network analysis and visualization in over 300 studies. To address emerging challenges, including multi‐factor experimental designs, multi‐treatment conditions, and multi‐omics data, we present a comprehensive upgrade with four key components: (1) A microbial co‐occurrence network pipeline integrating network computation (Pearson/Spearman/SparCC correlations), visualization, topological characterization of network and node properties, multi‐network comparison with statistical testing, network stability (robustness) analysis, and module identification and analysis; (2) Network mining functions for multi‐factor, multi‐treatment, and spatiotemporal‐scale analysis, including Facet.Network() and module.compare.m.ts(); (3) Transkingdom network construction using microbiota, multi‐omics, and other relevant data, with diverse visualization layouts such as MatCorPlot2() and cor_link3(); and (4) Transkingdom and multi‐omics network analysis, including corBionetwork.st() and visualization algorithms tailored for complex network exploration, including model_maptree2(), model_Gephi.3(), and cir.squ(). The updates in ggClusterNet 2 enable researchers to explore complex network interactions, offering a robust, efficient, user‐friendly, reproducible, and visually versatile tool for microbial co‐occurrence networks and indicator correlation patterns. The ggClusterNet 2R package is open‐source and available on GitHub (https://github.com/taowenmicro/ggClusterNet).
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spelling doaj-art-a10f1b3080c84184b409d70efee0af632025-08-20T02:24:23ZengWileyiMeta2770-596X2025-06-0143n/an/a10.1002/imt2.70041ggClusterNet 2: An R package for microbial co‐occurrence networks and associated indicator correlation patternsTao Wen0Yong‐Xin Liu1Lanlan Liu2Guoqing Niu3Zhexu Ding4Xinyang Teng5Jie Ma6Ying Liu7Shengdie Yang8Penghao Xie9Tianjiao Zhang10Lei Wang11Zhanyuan Lu12Qirong Shen13Jun Yuan14Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaGenome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaInner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Key Laboratory of Black Soil Protection and Utilization (Hohhot), Ministry of Agriculture and Rural Affairs Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control Hohhot ChinaInner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Key Laboratory of Black Soil Protection and Utilization (Hohhot), Ministry of Agriculture and Rural Affairs Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control Hohhot ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaNational Agricultural Experimental Station for Agricultural Environment, Luhe Jiangsu Academy of Agricultural Sciences Nanjing ChinaInner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Key Laboratory of Black Soil Protection and Utilization (Hohhot), Ministry of Agriculture and Rural Affairs Inner Mongolia Key Laboratory of Degradation Farmland Ecological Restoration and Pollution Control Hohhot ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource‐saving Fertilizers, Key Laboratory of Green Intelligent Fertilizer Innovation Nanjing Agricultural University Nanjing ChinaAbstract Since its initial release in 2022, ggClusterNet has become a vital tool for microbiome research, enabling microbial co‐occurrence network analysis and visualization in over 300 studies. To address emerging challenges, including multi‐factor experimental designs, multi‐treatment conditions, and multi‐omics data, we present a comprehensive upgrade with four key components: (1) A microbial co‐occurrence network pipeline integrating network computation (Pearson/Spearman/SparCC correlations), visualization, topological characterization of network and node properties, multi‐network comparison with statistical testing, network stability (robustness) analysis, and module identification and analysis; (2) Network mining functions for multi‐factor, multi‐treatment, and spatiotemporal‐scale analysis, including Facet.Network() and module.compare.m.ts(); (3) Transkingdom network construction using microbiota, multi‐omics, and other relevant data, with diverse visualization layouts such as MatCorPlot2() and cor_link3(); and (4) Transkingdom and multi‐omics network analysis, including corBionetwork.st() and visualization algorithms tailored for complex network exploration, including model_maptree2(), model_Gephi.3(), and cir.squ(). The updates in ggClusterNet 2 enable researchers to explore complex network interactions, offering a robust, efficient, user‐friendly, reproducible, and visually versatile tool for microbial co‐occurrence networks and indicator correlation patterns. The ggClusterNet 2R package is open‐source and available on GitHub (https://github.com/taowenmicro/ggClusterNet).https://doi.org/10.1002/imt2.70041microbial co‐occurrence networksmodularitymulti‐omics networkmulti‐network comparisonnetwork visualizationtranskingdom networks
spellingShingle Tao Wen
Yong‐Xin Liu
Lanlan Liu
Guoqing Niu
Zhexu Ding
Xinyang Teng
Jie Ma
Ying Liu
Shengdie Yang
Penghao Xie
Tianjiao Zhang
Lei Wang
Zhanyuan Lu
Qirong Shen
Jun Yuan
ggClusterNet 2: An R package for microbial co‐occurrence networks and associated indicator correlation patterns
iMeta
microbial co‐occurrence networks
modularity
multi‐omics network
multi‐network comparison
network visualization
transkingdom networks
title ggClusterNet 2: An R package for microbial co‐occurrence networks and associated indicator correlation patterns
title_full ggClusterNet 2: An R package for microbial co‐occurrence networks and associated indicator correlation patterns
title_fullStr ggClusterNet 2: An R package for microbial co‐occurrence networks and associated indicator correlation patterns
title_full_unstemmed ggClusterNet 2: An R package for microbial co‐occurrence networks and associated indicator correlation patterns
title_short ggClusterNet 2: An R package for microbial co‐occurrence networks and associated indicator correlation patterns
title_sort ggclusternet 2 an r package for microbial co occurrence networks and associated indicator correlation patterns
topic microbial co‐occurrence networks
modularity
multi‐omics network
multi‐network comparison
network visualization
transkingdom networks
url https://doi.org/10.1002/imt2.70041
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