Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms
A reverse phase HPLC method is described for the determination of esmolol hydrochloride in bulk and injections. Chromatography was carried on a C18 column using a mixture of acetonitrile, 0.05 M sodium acetate buffer and glacial acetic acid (35:65:3 v/v/v) as the mobile phase at a flow rate of 1 mL/...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/470785 |
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| _version_ | 1849398597206933504 |
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| author | Vanita Somasekhar D. Gowri Sankar |
| author_facet | Vanita Somasekhar D. Gowri Sankar |
| author_sort | Vanita Somasekhar |
| collection | DOAJ |
| description | A reverse phase HPLC method is described for the determination of esmolol hydrochloride in bulk and injections. Chromatography was carried on a C18 column using a mixture of acetonitrile, 0.05 M sodium acetate buffer and glacial acetic acid (35:65:3 v/v/v) as the mobile phase at a flow rate of 1 mL/min with detection at 275 nm. The retention time of the drug was 4.76 min. The detector response was linear in the concentration of 1-50 μg/mL. The limit of detection and limit of quantification was 0.614 and 1.86 μg/mL respectively. The method was validated by determining its sensitivity, linearity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of esmolol hydrochloride in bulk and injections. |
| format | Article |
| id | doaj-art-a10e1c96bb1a4483afc8cc2ab0fda0d7 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-a10e1c96bb1a4483afc8cc2ab0fda0d72025-08-20T03:38:34ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017380781210.1155/2010/470785Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage FormsVanita Somasekhar0D. Gowri Sankar1K.L.E Universityʼs College of Pharmacy, II Block, Rajajinagar, Bangalore-560010, IndiaCollege of Pharmaceutical Sciences, Andhra University, Visakhapatnam-530003, IndiaA reverse phase HPLC method is described for the determination of esmolol hydrochloride in bulk and injections. Chromatography was carried on a C18 column using a mixture of acetonitrile, 0.05 M sodium acetate buffer and glacial acetic acid (35:65:3 v/v/v) as the mobile phase at a flow rate of 1 mL/min with detection at 275 nm. The retention time of the drug was 4.76 min. The detector response was linear in the concentration of 1-50 μg/mL. The limit of detection and limit of quantification was 0.614 and 1.86 μg/mL respectively. The method was validated by determining its sensitivity, linearity, accuracy and precision. The proposed method is simple, economical, fast, accurate and precise and hence can be applied for routine quality control of esmolol hydrochloride in bulk and injections.http://dx.doi.org/10.1155/2010/470785 |
| spellingShingle | Vanita Somasekhar D. Gowri Sankar Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms E-Journal of Chemistry |
| title | Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms |
| title_full | Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms |
| title_fullStr | Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms |
| title_full_unstemmed | Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms |
| title_short | Development and Validation of a Rapid RP-HPLC Method for the Estimation of Esmolol Hydrochloride in Bulk and Pharmaceutical Dosage Forms |
| title_sort | development and validation of a rapid rp hplc method for the estimation of esmolol hydrochloride in bulk and pharmaceutical dosage forms |
| url | http://dx.doi.org/10.1155/2010/470785 |
| work_keys_str_mv | AT vanitasomasekhar developmentandvalidationofarapidrphplcmethodfortheestimationofesmololhydrochlorideinbulkandpharmaceuticaldosageforms AT dgowrisankar developmentandvalidationofarapidrphplcmethodfortheestimationofesmololhydrochlorideinbulkandpharmaceuticaldosageforms |