Morphological and Histological Analysis of the Gastrointestinal Systems in <i>Triplophysa strauchii</i> and <i>Triplophysa tenuis</i>: Insights into Digestive Adaptations
Fish are vital for material cycling and energy flow in aquatic ecosystems. The genus <i>Triplophysa</i>, with over 100 known species, is significant in the Central Asian highlands’ freshwater ecosystems. <i>T. strauchii</i> and <i>T. tenuis</i>, as representatives...
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| author | Zhengwei Wang Lirong Zhang Jie Wei Huimin Hao Syeda Maira Hamid Shixin Gao Wenjun Li Zhulan Nie |
| author_facet | Zhengwei Wang Lirong Zhang Jie Wei Huimin Hao Syeda Maira Hamid Shixin Gao Wenjun Li Zhulan Nie |
| author_sort | Zhengwei Wang |
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| description | Fish are vital for material cycling and energy flow in aquatic ecosystems. The genus <i>Triplophysa</i>, with over 100 known species, is significant in the Central Asian highlands’ freshwater ecosystems. <i>T. strauchii</i> and <i>T. tenuis</i>, as representatives, occupy distinct ecological niches and face challenges from climate change and human activities. There is a lack of research on <i>Triplophysa</i> fishes’ digestive systems, especially comparative studies, so this research aims to fill this gap. In September 2024, 40 samples of <i>T. strauchii</i> were collected from Sayram Lake and 40 samples of <i>T. tenuis</i> were collected from the Muzat River in Xinjiang. After acclimation, morphological observations (measuring fish and digestive tract parameters) and histological analyses (paraffin sectioning, HE staining, and microscopy) were carried out. The data were sorted in Excel and analyzed with an independent samples <i>t</i>-test in SPSS 27.0. Morphologically, <i>T. strauchii</i> has an obtuse snout, terminal mouth, specific upper lip papillae, and an S-shaped intestine about (1.45 ± 0.11) times its body length, while <i>T. tenuis</i> has an arc-shaped subterminal mouth, fringed papillae, and a spiral-shaped intestine around (0.82 ± 0.09) times its body length. Both possess a digestive tract, glands, and a hepatopancreas attached to the mesentery. Histologically, a large number of club cells were found in the oropharyngeal cavities of both species; their secretions have an adhesive effect on food, aiding food selection. Their digestive systems vary in structure and cell composition: the oropharyngeal cavity has three layers; the esophagus has four layers with more goblet cells in <i>T. strauchii</i>; the stomach has three regions without goblet cells and a thicker muscular layer in <i>T. strauchii</i>; the intestinal wall has four layers with different villi and goblet cell distributions; the hepatopancreas has lobules; and <i>T. strauchii</i> has a typical portal area. In conclusion, this study systematically compared the gastrointestinal systems of <i>T. strauchii</i> and <i>T. tenuis</i> for the first time, revealing significant structural differences related to their niches and feeding patterns as adaptations to specific environments. It fills the research gap, provides a basis for exploring fish ecological adaptation and environmental impacts on digestion, offers new ideas for <i>Triplophysa</i> protection strategies, and guides fish evolutionary biology research and <i>Triplophysa</i> resource protection and utilization. |
| format | Article |
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| spelling | doaj-art-a10dc4d29f9e4a8ca522424ba0c1cb572025-08-20T02:28:19ZengMDPI AGAnimals2076-26152025-04-01158109510.3390/ani15081095Morphological and Histological Analysis of the Gastrointestinal Systems in <i>Triplophysa strauchii</i> and <i>Triplophysa tenuis</i>: Insights into Digestive AdaptationsZhengwei Wang0Lirong Zhang1Jie Wei2Huimin Hao3Syeda Maira Hamid4Shixin Gao5Wenjun Li6Zhulan Nie7College of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaCollege of Life Science and Technology, Tarim University, Alar 843300, ChinaFish are vital for material cycling and energy flow in aquatic ecosystems. The genus <i>Triplophysa</i>, with over 100 known species, is significant in the Central Asian highlands’ freshwater ecosystems. <i>T. strauchii</i> and <i>T. tenuis</i>, as representatives, occupy distinct ecological niches and face challenges from climate change and human activities. There is a lack of research on <i>Triplophysa</i> fishes’ digestive systems, especially comparative studies, so this research aims to fill this gap. In September 2024, 40 samples of <i>T. strauchii</i> were collected from Sayram Lake and 40 samples of <i>T. tenuis</i> were collected from the Muzat River in Xinjiang. After acclimation, morphological observations (measuring fish and digestive tract parameters) and histological analyses (paraffin sectioning, HE staining, and microscopy) were carried out. The data were sorted in Excel and analyzed with an independent samples <i>t</i>-test in SPSS 27.0. Morphologically, <i>T. strauchii</i> has an obtuse snout, terminal mouth, specific upper lip papillae, and an S-shaped intestine about (1.45 ± 0.11) times its body length, while <i>T. tenuis</i> has an arc-shaped subterminal mouth, fringed papillae, and a spiral-shaped intestine around (0.82 ± 0.09) times its body length. Both possess a digestive tract, glands, and a hepatopancreas attached to the mesentery. Histologically, a large number of club cells were found in the oropharyngeal cavities of both species; their secretions have an adhesive effect on food, aiding food selection. Their digestive systems vary in structure and cell composition: the oropharyngeal cavity has three layers; the esophagus has four layers with more goblet cells in <i>T. strauchii</i>; the stomach has three regions without goblet cells and a thicker muscular layer in <i>T. strauchii</i>; the intestinal wall has four layers with different villi and goblet cell distributions; the hepatopancreas has lobules; and <i>T. strauchii</i> has a typical portal area. In conclusion, this study systematically compared the gastrointestinal systems of <i>T. strauchii</i> and <i>T. tenuis</i> for the first time, revealing significant structural differences related to their niches and feeding patterns as adaptations to specific environments. It fills the research gap, provides a basis for exploring fish ecological adaptation and environmental impacts on digestion, offers new ideas for <i>Triplophysa</i> protection strategies, and guides fish evolutionary biology research and <i>Triplophysa</i> resource protection and utilization.https://www.mdpi.com/2076-2615/15/8/1095<i>Triplophysa strauchii</i><i>Triplophysa tenuis</i>digestive systemmorphologyhistologyfeeding habit |
| spellingShingle | Zhengwei Wang Lirong Zhang Jie Wei Huimin Hao Syeda Maira Hamid Shixin Gao Wenjun Li Zhulan Nie Morphological and Histological Analysis of the Gastrointestinal Systems in <i>Triplophysa strauchii</i> and <i>Triplophysa tenuis</i>: Insights into Digestive Adaptations Animals <i>Triplophysa strauchii</i> <i>Triplophysa tenuis</i> digestive system morphology histology feeding habit |
| title | Morphological and Histological Analysis of the Gastrointestinal Systems in <i>Triplophysa strauchii</i> and <i>Triplophysa tenuis</i>: Insights into Digestive Adaptations |
| title_full | Morphological and Histological Analysis of the Gastrointestinal Systems in <i>Triplophysa strauchii</i> and <i>Triplophysa tenuis</i>: Insights into Digestive Adaptations |
| title_fullStr | Morphological and Histological Analysis of the Gastrointestinal Systems in <i>Triplophysa strauchii</i> and <i>Triplophysa tenuis</i>: Insights into Digestive Adaptations |
| title_full_unstemmed | Morphological and Histological Analysis of the Gastrointestinal Systems in <i>Triplophysa strauchii</i> and <i>Triplophysa tenuis</i>: Insights into Digestive Adaptations |
| title_short | Morphological and Histological Analysis of the Gastrointestinal Systems in <i>Triplophysa strauchii</i> and <i>Triplophysa tenuis</i>: Insights into Digestive Adaptations |
| title_sort | morphological and histological analysis of the gastrointestinal systems in i triplophysa strauchii i and i triplophysa tenuis i insights into digestive adaptations |
| topic | <i>Triplophysa strauchii</i> <i>Triplophysa tenuis</i> digestive system morphology histology feeding habit |
| url | https://www.mdpi.com/2076-2615/15/8/1095 |
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